A5026216929- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Systemic Sclerosis and Related Diseases
- Gastroesophageal reflux and treatments
- Eosinophilic Esophagitis
- Inflammatory Myopathies and Dermatomyositis
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Helicobacter pylori-related gastroenterology studies
- Atherosclerosis and Cardiovascular Diseases
- Celiac Disease Research and Management
- Platelet Disorders and Treatments
- Immune cells in cancer
- Rheumatoid Arthritis Research and Therapies
- Peripheral Neuropathies and Disorders
- Microscopic Colitis
- Renal Diseases and Glomerulopathies
- Diabetes and associated disorders
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Salivary Gland Disorders and Functions
- Mast cells and histamine
- Inflammasome and immune disorders
- T-cell and B-cell Immunology
- Protein Tyrosine Phosphatases
- Dysphagia Assessment and Management
Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán
2016-2025
Universidad Nacional Autónoma de México
2023
Center for Rheumatology
2021
University Health Network
2016
University of Toronto
2016
Mount Sinai Hospital
2016
Toronto General Hospital
2016
Antiphospholipid antibodies (aPL), especially those targeting β2 -glycoprotein I (β2 GPI), are well known to activate endothelial cells, monocytes, and platelets, with prothrombotic implications. In contrast, the interaction of aPL neutrophils has not been extensively studied. Neutrophil extracellular traps (NETs) have recently recognized as an important activator coagulation cascade, integral component arterial venous thrombi. This study was undertaken determine whether release NETs,...
Idiopathic achalasia is a disease of unknown etiology. The loss myenteric plexus associated with inflammatory infiltrates and autoantibodies support the hypothesis an autoimmune mechanism. Thirty-two patients diagnosed by high-resolution manometry were included. Twenty-six specimens from lower esophageal sphincter muscle compared 5 esophagectomy biopsies (control). Immunohistochemical (biopsies) flow cytometry (peripheral blood) analyses performed. Circulating anti-myenteric evaluated...
Patients with antiphospholipid syndrome (APS) are at risk for subclinical endothelial injury, as well accelerated atherosclerosis. In the related disease systemic lupus erythematosus, there is a well-established defect in circulating progenitors, which leads to an accrual of damage over time. This has been least partially attributed exaggerated expression type I interferons (IFNs). We sought determine whether these pathways important APS.We studied 68 patients primary APS. Endothelial...
The coronavirus disease 2019 (COVID-19) is related to enhanced production of NETs, and autoimmune/autoinflammatory phenomena. We evaluated the proportion low-density granulocytes (LDG) by flow cytometry, their capacity produce NETs was compared with that conventional neutrophils. protein cargo were quantified confocal microscopy ELISA. Antinuclear antibodies (ANA), anti-neutrophil cytoplasmic (ANCA) degradation addressed in serum. MILLIPLEX assay used assess cytokine levels macrophages’...
Currently, there is scant information regarding the features associated to persistence of post-COVID-19 syndrome, which main aim present study.A cohort study 102 COVID-19 patients was conducted. The symptoms were assessed by a standardised questionnaire. Lymphocyte immunophenotyping performed flow cytometry and chemokines/cytokines, neutrophil extracellular traps, tripartite motif 63, anti-cellular, anti-SARS-CoV-2 IgG antibodies addressed in serum. primary outcome syndrome after six months...
Objective. The incidence of thrombosis in patients with systemic lupus erythematosus (SLE) is 25 to 50-fold higher than the general population; we aimed define characteristics venous thrombotic events (VTE) and arterial (ATE) identify at highest risk. Methods. study included 219 recent-onset SLE. At baseline, standardized medical history laboratory tests were done. Followup visits occurred quarterly, information about damage accrual, comorbidities, cardiovascular risk factors was updated...
Abstract Background and Aim Idiopathic achalasia is a rare esophageal motor disorder. The disease state manifests local systemic inflammation, it appears that an autoimmune component specific autoantibodies participate in the pathogenesis. study aims to determine prevalence of chronic inflammatory diseases patients with compare results those from gastroesophageal reflux (GERD). Methods It was cross‐sectional included 114 idiopathic age‐matched sex‐matched control GERD. Data on presence...
The intramuscular administration of polymerized type I collagen (PTIC) for adult symptomatic COVID-19 outpatients downregulated hyperinflammation and improved symptoms. We inferred that LAIR1 is a potential receptor PTIC. Thus, binding assay surface plasmon resonance were performed to estimate the affinity interaction between M1 macrophages derived from THP-1 cells cultured with 2–10% PTIC 24 h. Lysates PTIC-treated cells, macrophage-like (MLCs), M1, + IFN-γ, LPS analyzed by Western blot...
Systemic sclerosis (SSc) shows variable clinical expression among different ethnic groups. Herein, we describe the features, prevalence of organ involvement, and autoantibody profile in Mexican Mestizo SSc patients compare them with from other groups.We included 139 patients. They underwent evaluation were tested for antinuclear antibodies (ANA), anticentromere (ACA), anti-topoisomerase I, anti-RNA polymerase III, anti-U1 RNP, anti-U3 anti-U11/U12 anti-Th/To, anti-PM-Scl, anti-Ku,...
Abstract Background Colchicine is an available, safe, and effective anti-inflammatory drug has been suggested as a COVID-19 treatment, but its usefulness in hospitalized severe patients not thoroughly demonstrated. Objective To address the safety efficacy of colchicine with COVID-19. Design We conducted triple-blind parallel non-stratified placebo-controlled clinical trial. Participants recruited 116 Mexico. Interventions Patients were randomized to receive 1.5 mg or placebo at time...
To assess the utility of interferon-α (IFN-α) in serum and cerebrospinal fluid (CSF) as a biomarker disease activity central neuropsychiatric systemic lupus erythematosus (cNPSLE).Serum CSF samples were drawn at hospitalization 34 patients with cNPSLE, 16 surgical SLE, 4 primary conditions, 25 nonautoimmune except 44 non-NPSLE whom only was studied. Six months later, serum/CSF taken 20 cNPSLE 35 patients, respectively. SLE assessed hospitalization, 6 later patients. IFN-α detected by Luminex...
Objectives . Polymerized-type I collagen (polymerized collagen) is a downmodulator of inflammation and cartilage regenerator biodrug. Aim To evaluate the effect intraarticular injections polymerized after arthroscopic lavage on clinical improvement in patients with knee osteoarthritis (OA). Methods Patients (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn>19</mml:mn></mml:mrow></mml:math>) were treated 6 2 mL...
Human leukocyte antigen (HLA) polymorphism studies in Systemic Sclerosis (SSc) have yielded variable results. These need to consider the genetic admixture of studied population. Here we used our previously reported definition Mexicans using HLA class I and II DNA blocks map susceptibility develop SSc its complications.We included 159 patients from a cohort Mexican Mestizo patients. We performed clinical evaluation, obtained SSc-associated antibodies, determined alleles sequence-based,...
To determine if cognitive dysfunction in patients with systemic lupus erythematosus (SLE) derives from an inflammatory process continuing disease activity, and increased levels of autoantibodies molecules serum cerebrospinal fluid (CSF).100 randomly selected participating inception SLE cohort were studied. At entry into the cohort, a standardized medical history extensive laboratory tests profile, including completed. Follow-up occurred every 3-6 months assessment characteristics,...
Monocytes and toll-like receptors (TLR) have been found in the inflammatory infiltrate of muscle biopsies patients with idiopathic myopathies (IIM), suggesting an important role these cells pathogenesis myositis. The monocyte subsets, their TLR expression peripheral blood relationship clinical characteristics IIM has not addressed.We recruited 45 diagnosis 15 age sex-adjusted healthy controls. We assessed disease activity damage, performed a nailfold capillaroscopy registered...
Objective The purpose of this study was to assess the utility tumor necrosis factor (TNF)-like weak inducer apoptosis (TWEAK) in serum and cerebrospinal fluid (CSF) as a biomarker neuropsychiatric systemic lupus erythematosus (NPSLE). Methods Thirty three NPSLE patients were evaluated at hospitalization six months later. As controls, five SLE with septic meningitis, 51 hospitalized without history (NP) manifestations infections, 16 NP (surgical-SLE), four primary disorders, 25 non-autoimmune...
Biomarkers for disease activity and damage accrual in idiopathic inflammatory myopathies (IIMs) are currently lacking. The purpose of this cross-sectional study is to analyze the relationship among low-density granulocytes (LDGs), neutrophil extracellular traps (NETs), clinical immunological features patients with IIM.We assessed activity, accrual, amount LDGs, NETs, expression LL-37, serum cytokines 65 adult IIM. Differences between groups correlations were by Kruskal-Wallis, Mann-Whitney...
We aimed to evaluate the usefulness of anti-α-fodrin antibodies (AFA) in combination with rheumatoid factor (RF) and/or antinuclear (ANA) as an alternative immunological criterion for Sjögren syndrome (SS) among patients negative anti-Ro/La serology.The study included 350 (100 arthritis, systemic lupus erythematosus, and sclerosis, 50 primary SS) randomly selected assessed SS. All were tested ANA, RF, anti-SSA/SSB, AFA antibodies. SS diagnosis was made on a clinical basis by 2...
Serum anti-myenteric autoantibodies define autoimmune achalasia and tissue MMP-9 activity may locally process autoantigenic proteins in the muscle of lower esophageal sphincter (LES) patients.Biopsies LES from 36 patients, 6 esophagogastric junction outflow obstruction (EGJOO) 16 transplant donors (TD) were compared a blind cross-sectional study. Histological characteristics such as inflammation, fibrosis, presence ganglion cells, cells Cajal, GAD65, PNMA2, S-100, P substance, proteoforms...