DNA topoisomerase (topo) II catalyses topological genomic changes essential for many metabolic processes. It is also regarded as a structural component of the nuclear matrix in interphase and mitotic chromosome scaffold. Mammals have two isoforms (alpha beta) with similar properties vitro. Here, we investigated their living proliferating cells, stably expressing biofluorescent chimera human isozymes. Topo IIalpha IIbeta behaved similarly but differently mitosis, where only topo was...

The effects of intake dietary amounts carrot or corresponding (−)-(3R)-falcarinol from carrots on development azoxymethane (AOM)-induced colon preneoplastic lesions were examined in male BDIX rats. Three groups eight AOM-treated rats fed the standard rat feed Altromin supplemented with either 10% (w/w) freeze-dried a natural content 35 μg falcarinol/g, maize starch to which was added falcarinol/g purified carrots, (control). After 18 weeks, animals euthanized and for tumors aberrant crypt...

The aim was to determine the polyp detection rate and per-patient sensitivity for polyps > 9 mm of colon capsule endoscopy (CCE) compared with colonoscopy as well diagnostic accuracy CCE.Individuals who had a positive immunochemical faecal occult blood test during screening investigator blinded CCE colonoscopy. Participants underwent repeat if significant lesions detected by were considered have been missed colonoscopy.There 253 participants. significantly higher in (P = 0.02). 87% (95% CI:...

Falcarinol (FaOH) and falcarindiol (FaDOH) are cytotoxic anti-inflammatory polyacetylenic oxylipins, which commonly found in the carrot family (Apiaceae). FaOH FaDOH have previously demonstrated a chemopreventive effect on precursor lesions of colorectal cancer (CRC) azoxymethane (AOM)-induced rats. The purpose present study was to elucidate possible mechanisms action for preventive precancerous determine how this dependent dose. Gene expression studies performed by RT-qPCR selected...

Background: The standard investigation in colorectal cancer screening (optical colonoscopy [OC]) has a less invasive alternative with the colon capsule endoscopy (CCE). experiences of individuals are needed to support decision aid (DA) and provide patient view future health technology assessments (HTA). We aimed explore CCE at home OC an outpatient clinic by participants who experienced both investigations on same bowel preparation.Methods: In mixed methods study, Danish positive...

To investigate whether the prokinetic prucalopride increases completion rate of colon capsule endoscopy (CCE). Secondary outcomes included demographic distribution, polyp detection (PDR), distribution Leighton-Rex grade, and adverse events.In a nested cohort within CareForColon2015 trial, subgroup 406 individuals underwent CCE in 2021. The first half (control) received standard bowel preparation second (prucalopride) was supplemented with 2 mg prucalopride. Transit times preparations were...

Background Colonoscopy is among the standard tests for colorectal cancer (CRC) screening. However, uptake varies, and alternatives such as colon capsule endoscopy (CCE) are available. The detection rate of clinically significant neoplasia with CCE, compared colonoscopy, remain unclear in this setting. Objective primary objective study was to compare rates advanced between CCE using a pathway which group could choose two procedures, while control offered only colonoscopy. Design A randomised,...

In the present study, we show that depletion of acyl-CoA-binding protein, Acb1p, in yeast affects ceramide levels, protein trafficking, vacuole fusion and structure. Vacuoles Acb1p-depleted cells are multi-lobed, contain significantly less SNAREs (soluble N-ethylmaleimide-sensitive attachment receptors) Nyv1p, Vam3p Vti1p, unable to fuse vitro. Mass spectrometric analysis revealed a dramatic reduction content ceramides whole-cell lipids vacuoles isolated from cells. Maturation aminopeptidase...

<b>Background and study aims: </b>Accurate lymph node staging is essential for the selection of an optimal treatment in patients with upper gastrointestinal cancer. Endoscopic ultrasound (EUS) fine-needle aspiration (FNA) are considered to be most accurate method locoregional staging. sonoelastography (ESE) assesses elasticity nodes has been used differentiate promising results. The aim this was evaluate use EUS, EUS – FNA, ESE, ESE-strain ratio using histology as gold standard. <b>Patients...

Falcarinol (FaOH) and falcarindiol (FaDOH) are found in many food plants of the Apiaceae family. Carrots a major dietary source these polyacetylenes. Feeding azoxymethane (AOM)-induced rats with carrots purified FaOH have previously been shown to inhibit neoplastic transformations colon. FaDOH also synergistic effect vitro, resulting significant increased cytotoxic activity. Based on findings antineoplastic (purity > 99%) was investigated AOM-induced rat model. Twenty received diet...

Background Guidelines suggest computed tomography colonography (CTC) following incomplete optical colonoscopy (OC). Colon capsule endoscopies (CCE) have been suggested as an alternative, although completion rates unsatisfactory. Introduction of artificial intelligence (AI)‐based localization algorithms the camera capsules may enable identification CCE investigations overlapping with OCs. Objective The study aims to investigate relative sensitivity compared CTC OC, rate when combining results...

Resources used in surveillance colonoscopies are taking up an increasing proportion of colonoscopy capacity. Colon capsule endoscopy (CCE) is a promising technique for noninvasive investigation the colon. We aimed to investigate CCE as possible filter colonic with primary outcome reducing number colonoscopies.Patients scheduled follow-up were subjected and only supplemental conventional if significant pathology was detected or examination incomplete. Significant defined more than two small...

Carrots are consumed worldwide. Several meta-analysis studies on carrot consumption have indicated that carrots play a central role as protecting vegetable against development of different types cancers. A cancer-preventive is plausible because they the main dietary source bioactive polyacetylenic oxylipins falcarinol (FaOH) and falcarindiol (FaDOH), which shown anti-proliferative anti-inflammatory activity in numerous vitro studies. In addition, purified FaOH FaDOH have, recent colorectal...

Background and study aims The Danish CareForColon2015 trial, launched in 2020 as part of the Colorectal Cancer Screening program, is largest randomized controlled trial to date on colon capsule endoscopy (CCE). This paper presents interim analysis with objective ensuring safety patients intervention group evaluating clinical performance trial's predefined parameters. Patients methods We evaluated initial 234 CCEs according quality, safety, completion. participation rates preference...

In the present study, we investigated effect of anthocyanidins on human topoisomerases I and II its relevance for DNA integrity within cells. Anthocyanidins bearing vicinal hydroxy groups at B-ring (delphinidin, DEL; cyanidin, CY) were found to potently inhibit catalytic activity II, without discriminating between IIα IIβ isoforms. However, in contrast topoisomerase poisons, DEL CY did not stabilize covalent DNA-topoisomerase intermediates (cleavable complex) or II. Using recombinant I,...

The aim of this study was to develop a machine learning-based model classify bowel cleansing quality and test in comparison pixel analysis assessments by four colon capsule endoscopy (CCE) readers. A with cleanliness classes (unacceptable, poor, fair good) were developed CCE videos. Cleansing readers 41 videos from previous compared the results both models yielded pilot study. classified 47 % agreement averaged classification readers, as 32 model. difference more than one class detected 12...

Introduction The use of capsule endoscopy has become an approved method in small bowel diagnostics, but the same level integration is not seen large diagnostics. We will colon (CCE) as a filter test colorectal cancer (CRC) screening between faecal immunochemical (FIT) and colonoscopy. aim to investigate clinical performance, population acceptability, economic implications procedure large-scale trial. Methods analysis randomly allocate 124 214 Danish citizens eligible for participation...

The intermediate filament network in simple glandular epithelial cells predominantly consists of heterotypic complexes cytokeratin 8 (K8) and 18 (K18). In contrast to other cytokeratins, K8 K18 are persistently expressed during malignant transformation, but changes cell morphology accompanied by alterations the network. To study molecular changes, were purified from surgically removed colon cancer normal epithelia tissues. Western blotting amino acid sequencing revealed presence abundant...

BackgroundPopulation-based screening for colorectal cancer by a faecal immunochemical test (FIT) is recommended the European Union. Detectable haemoglobin can indicate neoplasia as well other conditions. A positive FIT predicts an increased risk of death from but might also predict all-cause mortality.MethodsA cohort participants was followed using Danish National Register Causes Death. Data were retrieved Colorectal Cancer Screening Database supplemented with concentrations. specific and...

The budding and fission of vesicles during membrane trafficking requires many proteins, including those that coat the vesicles, adaptor proteins recruit components coat, small GTPases initiate vesicle formation. In addition, formation in vitro is promoted by hydrolysis acyl-CoA lipid esters. mechanisms which these esters are directed to appropriate membranes vivo, their precise roles biogenesis, not yet understood. Here, we present first report on associated ACBP domain-containing protein-1...

(3R)-Falcarinol (FaOH) and (3R,8S)-falcarindiol (FaDOH) have previously been shown to reduce the number of neoplastic lesions growth rate polyps in colon azoxymethane (AOM) treated rats. Based on previous investigations, it appears that different mechanisms actions are involved antineoplastic effect FaOH FaDOH. One mechanism action may be related antibacterial FaDOH thus their gut microbiota. This study aimed determine microbiota composition AOM rats.Azoxymethane rats were fed either a...