A5085214107- Gut microbiota and health
- Immune Response and Inflammation
- Infant Nutrition and Health
- Probiotics and Fermented Foods
- Food Allergy and Anaphylaxis Research
- Antimicrobial Peptides and Activities
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Transgenic Plants and Applications
- Protein Hydrolysis and Bioactive Peptides
- Clostridium difficile and Clostridium perfringens research
- Fatty Acid Research and Health
- Glycosylation and Glycoproteins Research
- Pediatric health and respiratory diseases
- Proteins in Food Systems
- Allergic Rhinitis and Sensitization
- Microbial Metabolites in Food Biotechnology
- Digestive system and related health
- Escherichia coli research studies
- Neonatal Respiratory Health Research
- Antimicrobial Resistance in Staphylococcus
- Diet and metabolism studies
- Bacterial biofilms and quorum sensing
- Helicobacter pylori-related gastroenterology studies
- Monoclonal and Polyclonal Antibodies Research
University of Copenhagen
2015-2025
Frederiksberg Hospital
2017-2019
Technical University of Denmark
1998-2013
New York University Press
2010
Cambridge University Press
2010
Herlev Hospital
1996
Hvidovre Hospital
1996
Abstract Dendritic cells (DC) play a pivotal immunoregulatory role in the Th1, Th2, and Th3 cell balance are present throughout gastrointestinal tract. Thus, DC may be targets for modulation by gut microbes, including ingested probiotics. In study, we tested hypothesis that species of Lactobacillus, important members flora, differentially activate DC. Bone marrow-derived murine were exposed to various lethally irradiated Lactobacillus spp. resultant culture supernatants analyzed IL-6, IL-10,...
Objective To investigate whether a whole grain diet alters the gut microbiome and insulin sensitivity, as well biomarkers of metabolic health functionality. Design 60 Danish adults at risk developing syndrome were included in randomised cross-over trial with two 8-week dietary intervention periods comprising refined diet, separated by washout period ≥6 weeks. The response to interventions on composition sensitivity measures glucose lipid metabolism, functionality, inflammatory markers,...
A dietary shift from resource-demanding animal protein to sustainable food sources, such as protein-rich beans, lowers the climate footprint of production. In this study, we examined nutrients and antinutrients in 15 fava bean varieties cultivated Sweden select with high nutritional value. On a dry weight basis, beans were analyzed for their content (range 26–33%), amino acids (leucine range: 50.8–72.1 mg/g protein, lysine 44.8–74.8 protein), fiber (soluble fraction 0.55–1.06%, insoluble...
Saponins are naturally occurring amphiphilic molecules and have been associated with many biological activities. The aim of the present study was to investigate whether soya saponins trigger onset soyabean-induced enteritis in Atlantic salmon (Salmo salar L.), examine if dietary increase epithelial permeability distal intestine salmon. Seven experimental diets containing different levels were fed seawater-adapted for 53 d. included a fishmeal-based control diet, two added saponins, one diet...
Humans and other mammals coexist with a diverse array of microbes colonizing the intestine, termed microflora. The relationship is symbiotic, benefiting from stable environment nutrient supply, host gaining competitive exclusion pathogens continuously maintenance gut immune homeostasis. Here we report novel crosstalk mechanisms between human enterocyte cell line, Caco2, underlying monocyte-derived DC in transwell model where Gram-positive (G+) commensals prevent Toll-like receptor-4...
The current work aimed at tracing the causative components for soybean-induced enteritis in Atlantic salmon (Salmo salar L.). Soybean molasses was subjected to phase separation using n-butanol. Three subfractions were obtained as follows: butanol phase, precipitate, and water phase. biochemical composition of soybean analyzed detail: Protein, fat, ash quantified according standard methods. Sucrose, raffinose, stachyose high-performance anion-exchange chromatography. Soyasaponins...
Summary The gut microbiota is vital in the maintenance of homeostasis immune system. Its diversity and composition play major roles relation to allergies inflammatory bowel diseases, administration lactic acid bacteria (LAB), such as lactobacilli bifidobacteria, has positive effects on these pathologies. However, mechanisms behind beneficial are largely unknown. Here we reveal divergent played by Toll‐like receptor‐2 (TLR2) nucleotide‐binding oligomerization domain‐2 (NOD2) dendritic cell...
Adherence to a low-gluten diet has become increasingly common in parts of the general population. However, effects reducing gluten-rich food items including wheat, barley and rye cereals healthy adults are unclear. Here, we undertook randomised, controlled, cross-over trial involving 60 middle-aged Danish without known disorders with two 8-week interventions comparing (2 g gluten per day) high-gluten (18 day), separated by washout period at least six weeks habitual (12 day). We find that,...
Lactobacilli are probiotics that, among other health-promoting effects, have been ascribed immunostimulating and virus-preventive properties. Certain Lactobacillus spp. shown to possess strong interleukin-12 (IL-12) -inducing As IL-12 production depends on the up-regulation of type I interferons (IFNs), we hypothesized that IL-12-inducing capacity acidophilus NCFM in murine bone-marrow-derived dendritic cells (DCs) is caused by an IFN-β, which subsequently induces double-stranded RNA binding...
Methicillin-resistant Staphylococcus aureus (MRSA) continues to be a serious human pathogen, and particularly the spread of community associated (CA)-MRSA strains such as USA300 is concern, these can cause severe infections in otherwise healthy adults. Recently, we reported that cyclodepsipeptide termed Solonamide B isolated from marine bacterium, Photobacterium halotolerans strongly reduces expression RNAIII, effector molecule agr quorum sensing system. Here show interferes with binding S....
Abstract Billions of bacteria inhabit the gastrointestinal tract. Immune-microbial cross talk is responsible for immunological homeostasis, and symbiotic microbial species induce regulatory immunity, which helps to control inflammation levels. In this study we aimed identify within equine intestinal microbiota with potential immunity. These could be future targets preventing or treating low-grade chronic occurring as a result changes disruption homeostasis. 16S rRNA gene amplicon sequencing...
ABSTRACT The development and maintenance of immune homeostasis indispensably depend on signals from the gut flora. Lactic acid bacteria (LAB), which are gram-positive (G + ) organisms, plausible significant players have received much attention. Gram-negative − commensals, such as members family Enterobacteriaceae , may, however, be immunomodulators that important G organisms but tend to overlooked. Dendritic cells (DCs) crucial regulators, therefore, present study aimed at investigating...
Lactic acid bacteria (LAB) are abundant in the gastrointestinal tract where they continuously regulate immune system. NK cells potently activated by dendritic (DCs) matured inflammatory stimuli, and present gut epithelium mesenteric lymph nodes, but it is not known how NK-DC interactions affected predominantly non-pathogenic LAB. We demonstrate that human DCs exposed to different strains of gut-derived LAB consistently induce proliferation, cytotoxicity activation markers autologous cells....
Early-life interventions in the intestinal environment have previously been shown to influence diabetes incidence. We therefore hypothesized that a gluten-free (GF) diet, known decrease incidence of type 1 diabetes, would protect against development when fed only during pregnancy and lactation period. Pregnant nonobese diabetic (NOD) mice were GF or standard diet until all pups weaned diet. The early-life dramatically decreased insulitis. Gut microbiota analysis by 16S rRNA gene sequencing...
Abstract Preterm neonates are highly sensitive to systemic infections in early life but little is known about immune development following preterm birth. We hypothesized that have immature immunity with distinct developmental trajectory for the first several weeks of life, relative those born at near-term or term. Using pigs as a model, we characterized blood leukocyte subsets, antimicrobial activities and TLR-mediated cytokine production during after Relative term pigs, newborn had low...
Bovine β-lactoglobulin was hydrolyzed with trypsin or chymotrypsin before, during and after treatment at 600 MPa pH 6·8 for 10 min 30, 37 44 °C. The extent of hydrolysis under pressure noticeably higher than atmospheric pressure, particularly when used. Addition proteases ambient to previously pressure-treated gave only a modest increase in proteolysis respect the untreated protein. Products enzyme were separated by reverse-phase HPLC, found be different from those obtained residual...
Abstract n−3 PUFA influence immune functioning and may affect the cytokine phenotype during development. To examine whether maternal fish oil supplementation lactation could modify later responses in children, 122 lactating Danish mothers with a intake below population median were randomized to groups supplemented for first 4 mon of 4.5 g/d (equivalent 1.5 long‐chain PUFA) or olive oil. Fifty‐three highest quartile also included. The FA composition erythrocyte membranes was measured at 2 1/2...
Milk contains immunomodulatory compounds that may be important to protect the immature intestine in preterm neonates from harmful inflammatory reactions involved disorders like necrotising enterocolitis (NEC). We hypothesised bovine colostrum and milk formulas enriched with sialic acids (SL), gangliosides (Gang) or osteopontin (OPN) would improve gastrointestinal function NEC resistance neonates. Forty-seven caesarean-delivered pigs were given total parenteral nutrition for 2 d followed by...
Abstract Background Prebiotics are non-digestible food ingredients believed to beneficially affect host health by selectively stimulating the growth of beneficial bacteria residing in gut. Such have been reported protect against pathogenic infections. However, contradicting results on prevention Salmonella infections with prebiotics published. The aim present study was examine whether S . Typhimurium SL1344 infection mice could be prevented administration dietary carbohydrates different...