A5071830053- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Biosensors and Analytical Detection
- COVID-19 epidemiological studies
- Respiratory viral infections research
- Bacillus and Francisella bacterial research
- Infection Control and Ventilation
- Animal Virus Infections Studies
- Natural Language Processing Techniques
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Toxoplasma gondii Research Studies
- COVID-19 Impact on Reproduction
- Cytomegalovirus and herpesvirus research
- Immunodeficiency and Autoimmune Disorders
- Vaccine Coverage and Hesitancy
- Advanced biosensing and bioanalysis techniques
- Biomedical Text Mining and Ontologies
- COVID-19 and Mental Health
- COVID-19 and healthcare impacts
- Mosquito-borne diseases and control
- Herpesvirus Infections and Treatments
- Hemophilia Treatment and Research
- Drug-Induced Adverse Reactions
Goethe University Frankfurt
2009-2024
University Hospital Frankfurt
2009-2024
TU Dresden
2024
Coronavirus in Travelers Returning from China A group of 126 German nationals was evacuated Wuhan to Frankfurt after screening for symptoms Covid-19 and undergo a 14-day quarantine a...
Abstract Due to numerous mutations in the spike protein, SARS-CoV-2 variant of concern Omicron (B.1.1.529) raises serious concerns since it may significantly limit antibody-mediated neutralization and increase risk reinfections. While a rapid number cases is being reported worldwide, until now there has been uncertainty about efficacy vaccinations monoclonal antibodies. Our vitro findings using authentic variants indicate that contrast currently circulating Delta variant, vaccine-elicited...
Omicron is the evolutionarily most distinct severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant of concern (VOC) to date. We report that BA.1 breakthrough infection in BNT162b2-vaccinated individuals resulted strong neutralizing activity against BA.1, BA.2, and previous SARS-CoV-2 VOCs but not sublineages BA.4 BA.5. induced a robust recall response, primarily expanding memory B (B
Due to globally rising numbers of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, resources for real-time reverse-transcription polymerase chain reaction (rRT-PCR)-based testing have been exhausted. In order meet the demands and reduce transmission, SARS-CoV-2 antigen-detecting rapid diagnostic tests (Ag-RDTs) are being considered. These fast, inexpensive, simple use, but whether they detect potentially infectious cases has not well studied. We evaluated three...
<h2>Summary</h2><h3>Background</h3> In recent months, Omicron variants of SARS-CoV-2 have become dominant in many regions the world, and case numbers with subvariants BA.1 BA.2 continue to increase. Due numerous mutations spike protein, efficacy currently available vaccines, which are based on Wuhan-Hu 1 isolate SARS-CoV-2, is reduced, leading breakthrough infections. Efficacy monoclonal antibody therapy also likely impaired. <h3>Methods</h3> our <i>in vitro</i> study using A549-AT cells...
The novel coronavirus SARS-CoV-2 is the causative agent of acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. Meanwhile, increased demand for testing led shortage reagents and supplies compromised performance diagnostic laboratories in many countries. Both World Health Organization (WHO) Center Disease Control Prevention (CDC) recommend multi-step RT-PCR assays using multiple primer probe pairs, might complicate interpretation test results,...
BNT162b2-vaccinated individuals after Omicron BA.1 breakthrough infection have strong serum-neutralizing activity against BA.1, BA.2, and previous SARS-CoV-2 variants of concern (VOCs) yet less the highly contagious sublineages BA.4 BA.5 that displaced variants. Because latter are derived from we characterized COVID-19 mRNA vaccine triple-immunized who experienced BA.2 infection. We demonstrate sera these broadly neutralizing VOCs all tested sublineages, including BA.2-derived BA.2.12.1...
Abstract Whether monoclonal antibodies are able to neutralize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern has been investigated using pseudoviruses. In this study we show that bamlanivimab, casirivimab, and imdevimab efficiently authentic SARS-CoV-2, including variant B.1.1.7 (alpha), but B.1.351 (beta) P.2 (zeta) were resistant against bamlanivimab partially casirivimab. variantshas We SARS-CoV-2 carrying E484K less susceptible convalescent vaccine-elicited sera.
Evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant has led to emergence sublineages with different patterns neutralizing antibody evasion. We report that BA.4/BA.5 breakthrough infection individuals immunized SARS-CoV-2 wild-type-strain-based mRNA vaccines results in a boost BA.4.6, BF.7, BQ.1.1, and BA.2.75 neutralization but does not efficiently BA.2.75.2, XBB, or XBB.1.5 neutralization. In silico analyses showed spike glycoprotein lost most B...
It has previously been observed for SARS and influenza that transmission may also occur among passengers seated beyond the 2-row perimeter, 2,3 indicating possible airborne transmission.Our findings do not rule out of SARS-CoV-2 in an airplane cabin.This study had several limitations.We did obtain information on crew were able to contact all passengers.We antibody tests from passengers.Additional transmissions have occurred remained undetected.
Abstract The IgG1 monoclonal antibody (mAb) bamlanivimab (LY-CoV555) prevents viral attachment and entry into human cells by blocking to the ACE2 receptor. However, whether is equally effective against SARS-CoV-2 emerging variants of concern (VOC) not fully known. Hence, aim this study was determine VOC. ability neutralize five including B.1.1.7 (mutations include N501Y del69/70), B.1.351 E484K N501Y) P.2 in absence a mutation) analyzed infectious cell culture using CaCo2 cells....
The novel coronavirus SARS-CoV-2 is the causative agent of acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. Laboratory work with in laboratory setting was rated biosafety level 3 (BSL-3) biocontainment level. However, certain research applications particular molecular biology require incomplete denaturation proteins, might cause safety issues handling contaminated samples. In this study, we evaluated lysis buffers that are commonly used...
In the pandemic, testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by real-time polymerase chain reaction is one of pillars on which countermeasures are based. Factors limiting output laboratories interfere with effectiveness public health measures. Conserving reagents pooling samples in low-probability settings proposed but may cause dilution and loss sensitivity. Blood transfusion services had experience performance high throughput nucleic acid (NAT) analysis can...
Human cytomegalovirus (CMV) is a significant cause of morbidity and mortality in patient groups at risk. We have previously shown that the anti-CMV IgG seroprevalence an urban region Germany has changed over last decades. Overall, decline from 63.7 to 57.25% had been observed between 1988-1997 1998-2008 (p < 0,001). Here, we continuously follow trends most recent decade 2009 2018. In retrospective analysis, determined CMV antibodies our cohort, stratified by gender selected risk (e.g.,...
Our study in 21 pediatric cancer patients demonstrates that 3 doses of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) messenger RNA vaccine (BioNTech/Pfizer) elicited both humoral and cellular immunity most during chemotherapy. Immunity was stronger children with solid tumors maintenance therapy compared to those hematological malignancies or intensive Clinical Trials Registration.ȃGerman Registry for (DRKS00025254).
Abstract The novel coronavirus SARS-CoV-2 is the causative agent of acute respiratory disease COVID-19 which has become a global concern due to its rapid spread. Meanwhile, increased demand in testing led shortage reagents, supplies, and compromised performance diagnostic laboratories many countries. Both world health organization (WHO) Center for Disease Control Prevention (CDC) recommend multi-step RT-PCR assays using multiple primer probe pairs, might complicate interpretation test...
Abstract Recently, we reported that BNT162b2-vaccinated individuals after Omicron BA.1 breakthrough infection have strong serum neutralizing activity against BA.1, BA.2, and previous SARS-CoV-2 variants of concern (VOCs), yet less the highly contagious sublineages BA.4 BA.5 displaced variants. As latter are derived from characterized COVID-19 mRNA vaccine triple-immunized who experienced BA.2 infection. We demonstrate sera these broadly VOCs as well all tested sublineages, including...
With the pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ongoing in Europe June 2020, day care centers were reopened state Hesse, Germany, after lockdown. The role young children play dynamics transmission was unknown.We conducted a longitudinal study over 12 weeks and days (18 2020-10 September 2020) to screen attendees staff from for both gastrointestinal shedding SARS-CoV-2. A total 859 (age range, 3 months-8 years) 376 members 50 centers, which chosen...
Abstract Background Rapid antigen tests for SARS-CoV-2 became available recently, offering an opportunity to vastly increase testing capacities. Antigen offer lower sensitivity than the gold standard, RT-PCR, but rapid sample-to-answer time. High-frequency with test may offset sensitivity, and can be done at-home collection of samples, potential benefit in screening efforts. In this study, we set out evaluate practical application self-performed high-frequency a school setting. Method A...
Testing for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by RT-PCR is a vital public health tool in the pandemic. Self-collected samples are increasingly used as an alternative to nasopharyngeal swabs. Several studies suggested that they sufficiently sensitive be useful alternative. However, there limited data directly comparing several different types of self-collected materials determine which material preferable. A total 102 predominantly symptomatic adults with confirmed...
Abstract The continued evolution of the SARS-CoV-2 Omicron variant has led to emergence numerous sublineages with different patterns evasion from neutralizing antibodies. We investigated activity in immune sera individuals vaccinated wild-type spike (S) glycoprotein-based COVID-19 mRNA vaccines after subsequent breakthrough infection BA.1, BA.2, or BA.4/BA.5 study antibody responses against high relevance. report that exposure infections sublineages, and especially BA.4/BA.5, results a boost...