A5031235666- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
- Single-cell and spatial transcriptomics
- Infectious Encephalopathies and Encephalitis
- Cell Image Analysis Techniques
- Immune Cell Function and Interaction
- Antimicrobial Resistance in Staphylococcus
- Pneumocystis jirovecii pneumonia detection and treatment
- Animal Virus Infections Studies
- Immune responses and vaccinations
- Intensive Care Unit Cognitive Disorders
- Neuroscience and Neural Engineering
- Mosquito-borne diseases and control
- Carbon Nanotubes in Composites
- Viral gastroenteritis research and epidemiology
- COVID-19 and Mental Health
- Immunodeficiency and Autoimmune Disorders
- Viral Infections and Vectors
- SARS-CoV-2 detection and testing
- Bacterial Identification and Susceptibility Testing
- Insect symbiosis and bacterial influences
- Advanced Biosensing Techniques and Applications
Colorado State University
2020-2025
RTI International
2024
University of Tennessee Health Science Center
2024
St. Jude Children's Research Hospital
2024
Museum of Heilongjiang Province
2021
South Asian University
2019
Mayo Clinic in Arizona
2019
RNA vaccines have demonstrated efficacy against SARS-CoV-2 in humans, and the technology is being leveraged for rapid emergency response. In this report, we assessed immunogenicity and, first time, toxicity, biodistribution, protective preclinical models of a two-dose self-amplifying messenger (SAM) vaccine, encoding prefusion-stabilized spike antigen Wuhan-Hu-1 strain delivered by lipid nanoparticles (LNPs). mice, one immunization with SAM vaccine elicited robust spike-specific antibody...
The mosquito midgut functions as a key interface between pathogen and vector. However, studies of physiology virus infection dynamics are scarce, in Culex tarsalis— an extremely efficient vector West Nile (WNV) — nonexistent. We performed single-cell RNA sequencing on Cx. tarsalis midguts, defined multiple cell types, determined whether specific types more permissive to WNV infection. identified 20 states comprising 8 distinct consistent with existing descriptions Drosophila Aedes aegypti...
SARS-CoV-2 has swept across the globe, causing millions of deaths worldwide. Though most survive, many experience symptoms COVID-19 for months after acute infection. Successful prevention and treatment infection its associated sequelae is dependent on in-depth knowledge viral pathology spectrum patient phenotypes physiologic responses. Longitudinal biobanking provides a valuable resource clinically integrated, easily accessed, quality-controlled samples researchers to study differential...
Abstract Jamaican fruit bats ( Artibeus jamaicensis ) naturally harbor a wide range of viruses human relevance. These infections are typically mild in bats, suggesting unique features their immune system. To better understand the response to viral we infected male with bat-derived influenza A virus (IAV) H18N11. Using comparative single-cell RNA sequencing, generated atlases bat intestine and mesentery. Gene expression profiling showed that H18N11 infection resulted moderate induction...
Abstract Immune response dysregulation plays a key role in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pathogenesis. In this study, we evaluated immune and endothelial blood cell profiles of patients with disease 2019 (COVID-19) to determine critical differences between those mild, moderate, or COVID-19 using spectral flow cytometry. We examined suite phenotypes, including monocytes, T cells, NK B neutrophils, alongside surface intracellular markers activation. Our results...
Introduction Post-acute sequelae of COVID-19 affects the quality life many survivors, yet etiology post-acute remains unknown. We aimed to determine if persistent inflammation and ongoing T-cell activation during convalescence were a contributing factor pathogenesis COVID-19. Methods evaluated 67 individuals diagnosed with by nasopharyngeal polymerase chain reaction for symptoms at separate time points occurring up 180 days post-diagnosis. Fifty-two these longitudinally. obtained whole blood...
The COVID-19 pandemic has generated intense interest in the rapid development and evaluation of vaccine candidates for this disease other emerging diseases. Several novel methods preparing are currently undergoing clinical response to urgent need prevent spread COVID-19. In many cases, these rely on new approaches production immune stimulation. We report use a method (SolaVAX) an inactivated candidate testing that hamster animal model its ability infection upon challenge with SARS-CoV-2...
The Nix-TB clinical trial evaluated a new 6 month regimen containing three oral drugs; bedaquiline (B), pretomanid (Pa), and linezolid (L) (BPaL regimen) for the treatment of tuberculosis (TB). This achieved remarkable results as almost 90% multidrug-resistant or extensively drug-resistant TB participants were cured but many patients also developed severe adverse events (AEs). AEs associated with long-term administration protein synthesis inhibitor linezolid. Spectinamide 1599 is...
SARS-CoV-2 has infected millions across the globe. Many individuals are left with persistent symptoms, termed post-acute sequelae of COVID-19 (PASC), for months after infection. Hyperinflammation in acute and convalescent stages emerged as a risk factor poor disease outcomes, this may be exacerbated by dietary inadequacies. Specifically, fatty acids powerful inflammatory mediators have significant role modulation.The major objective project was to pilot an investigation plasma acid (PFA)...
Uptake of polydispersed acid-functionalized single-walled carbon nanotubes (AF-SWCNTs) in resting and LPS-activated B cells was studied using fluorescence-tagged AF-SWCNTs (FAF-SWCNTs). Activated internalized substantially higher amounts FAF-SWCNTs [76.5% AF-SWCNT+ cells, mean fluorescence intensity (MFI) 720.6] as compared to the [39.5% MFI 198.5]. S G2/M phases were found have significantly uptake G0/G1 phase. Confocal microscopy indicated that essentially localized on cell membrane...
Convalescent plasma (CP) has been embraced as a safe therapeutic option for coronavirus disease 2019 (COVID-19), while other treatments are developed. Severe acute respiratory syndrome 2 (SARS-CoV-2) is not transmissible by transfusion, but bloodborne pathogens remain risk in regions with high endemic prevalence of disease. Pathogen reduction can mitigate this risk; thus, the objective study was to evaluate effect riboflavin and ultraviolet light (R + UV) pathogen technology on functional...
Abstract The mosquito midgut functions as a key interface between pathogen and vector. However, studies of physiology virus infection dynamics are scarce, in Culex tarsalis – an extremely efficient vector West Nile (WNV) nonexistent. We performed single-cell RNA sequencing on Cx. midguts, defined multiple cell types, determined whether specific types more permissive to WNV infection. identified 20 states comprising 8 distinct consistent with existing descriptions Drosophila Aedes aegypti...
The Nix-TB clinical trial evaluated a new 6 month regimen containing three oral drugs; bedaquiline (B), pretomanid (Pa), and linezolid (L) (BPaL regimen) for the treatment of tuberculosis (TB). This achieved remarkable results as almost 90% multidrug-resistant or extensively drug-resistant TB participants were cured but many patients also developed severe adverse events (AEs). AEs associated with long-term administration protein synthesis inhibitor linezolid. Spectinamide 1599 is...
Abstract Flow cytometers can now analyze up to 50 parameters per cell and millions of cells sample; however, conventional methods data are subjective time-consuming. To address these issues, we have developed a novel flow cytometry analysis pipeline identify plethora populations efficiently. Coupled with feature engineering immunological context, researchers immediately extrapolate discoveries through easy-to-understand plots. The R-based uses Fluorescence Minus One (FMO) controls or...
Flow cytometry allows the visualization of physical, functional, and/or biological properties cells including antigens, cytokines, size, and complexity. With increasingly large flow panels able to analyze up 50 parameters, there is a need standardize protocols achieve high-quality data that can be input into analysis algorithms. Without this clean data, algorithms may incorrectly categorize cell populations present in samples. In protocol, we outline comprehensive methodology prepare samples...
Abstract The Nix-TB clinical trial evaluated a new 6-month regimen containing three-oral- drugs; bedaquiline (B), pretomanid (Pa) and linezolid (L) (BPaL regimen) for treatment of tuberculosis (TB). This achieved remarkable results as almost 90% the multidrug resistant (MDR) or extensively drug (XDR) TB participants were cured but many patients also developed severe adverse events (AEs). AEs associated with long-term administration protein synthesis inhibitor linezolid. Spectinamide 1599 is...
Abstract The COVID-19 pandemic has generated intense interest in the rapid development and evaluation of vaccine candidates for this disease other emerging diseases. Several novel methods preparing are currently undergoing clinical response to urgent need prevent spread COVID-19. In many cases, these rely on new approaches production immune stimulation. We report use a method (SolaVAX™) an inactivated candidate testing that hamster animal model its ability infection upon challenge with...