A5065319547- Circadian rhythm and melatonin
- Adipose Tissue and Metabolism
- Hearing, Cochlea, Tinnitus, Genetics
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- Retinopathy of Prematurity Studies
- Barrier Structure and Function Studies
- Connexins and lens biology
- Redox biology and oxidative stress
- Photoreceptor and optogenetics research
- Microtubule and mitosis dynamics
- Erythrocyte Function and Pathophysiology
- Spaceflight effects on biology
- Metal and Thin Film Mechanics
- Proteoglycans and glycosaminoglycans research
- Wnt/β-catenin signaling in development and cancer
- Olfactory and Sensory Function Studies
- Copper Interconnects and Reliability
- Kruppel-like factors research
- Muscle Physiology and Disorders
- Plant nutrient uptake and metabolism
- Salivary Gland Disorders and Functions
- Cell Adhesion Molecules Research
- Ear and Head Tumors
- Cancer-related gene regulation
Cincinnati Children's Hospital Medical Center
2011-2024
John Wiley & Sons (Germany)
2018
University of Cincinnati
2013
University of Sussex
2007-2011
Almost all life forms can detect and decode light information for adaptive advantage. Examples include the visual system, in which photoreceptor signals are processed into virtual images, circadian entrains a physiological clock. Here we describe response pathway mice that employs encephalopsin (OPN3, 480 nm, blue-light-responsive opsin) to regulate function of adipocytes. Germline null adipocyte-specific conditional show light- Opn3-dependent deficit thermogenesis become hypothermic upon...
The two compositionally distinct extracellular cochlear fluids, endolymph and perilymph, are separated by tight junctions that outline the scala media reticular lamina. Mutations in TRIC (also known as MARVELD2), which encodes a tricellular junction protein tricellulin, lead to nonsyndromic hearing loss (DFNB49). We generated knockin mouse carries mutation orthologous coding linked DFNB49 humans. Tricellulin was absent from inner ear epithelia of mutant animals, developed rapidly progressing...
We established a conditional deletion of Aurora A kinase (AurA) in Cdk1 analogue-sensitive DT40 cells to analyze AurA knockout phenotypes after activation. In the absence AurA, form bipolar spindles but fail properly align their chromosomes and exit mitosis with segregation errors. The resulting daughter exhibit variety are highly aneuploid. B (AurB)–inhibited show similar chromosome alignment problem cytokinesis defects, binucleate cells. Conversely, lacking AurB activity without anaphase,...
Methionine sulfoxide reductases (MSR) are enzymes responsible for catalyzing the reduction of methionine-sulfoxides. We previously demonstrated that variants in human MSRB3, a member MSR family, associated with profound autosomal recessive prelingual non-syndromic hearing loss DFNB74. To better understand role MSRB3 auditory pathway, we generated complete MsrB3 gene knock-out mouse model DFNB74 deafness. The deficient showed by postnatal day 16 (P16), which was accompanied hair cell...
Normal development requires tight regulation of cell proliferation and death. Here, we have investigated these control mechanisms in the hyaloid vessels, a temporary vascular network mammalian eye that Wnt/β-catenin response for scheduled regression. We whether Wnt was linked to oncogene Myc, cyclin-dependent kinase inhibitor CDKN1A (P21), both established regulators cycle progression Our analysis showed pathway co-receptors LRP5 LRP6 overlapping activities mediate signaling endothelial...
Abstract Opsin-3 ( Opn3 , encephalopsin) was the first nonvisual opsin gene discovered in mammals. Since then, several functions have been described, and two cases (adipose tissue, smooth muscle) light sensing activity is implicated. In addition to peripheral tissues, robustly expressed within central nervous system, for which it derives its name. Despite this expression, no studies investigated developmental or adult CNS consequences of loss-of-function. Here, behavioral mice deficient were...
Ptprq is a receptor-like inositol lipid phosphatase associated with the shaft connectors of hair bundles. Three lines evidence suggest chondroitin sulfate proteoglycan: (1) chondroitinase ABC treatment causes loss ruthenium-red reactive, electron-dense particles connectors, (2) an increase in electrophoretic mobility Ptprq, and (3) bundles developing inner ear wild-type mice, but not those Ptprq(-/-) react monoclonal antibody (mAb) 473-HD, IgM that recognizes dermatan-sulfate-dependent...
Abstract Maturation of sensory systems in mammals is regulated by appropriate stimulation. Developmental refinement the eye and visual system light One compelling example that fetal mouse pups deprived exhibit altered vascular development their eyes. Previous work demonstrated activation photopigment melanopsin ( Opn4 ), an atypical opsin expressed intrinsically photosensitive retinal ganglion cells (ipRGCs), crucial to normal development. This suggested unusual hypothesis was ipRGC...
Almost all life forms can detect and decode light information for adaptive advantage. Examples include the visual system, where photoreceptor signals are processed into virtual images, circadian entrains a physiological clock. Here we describe pathway in mice that employs encephalopsin (OPN3, 480 nm responsive opsin) to mediate responses murine adipocytes. The adipocyte light-OPN3 regulates neonatal growth is required at least three important functions including (1) photoentrainment of local...
Summary Almost all life forms can decode light information for adaptive advantage. Examples include the visual system, where photoreceptor signals are interpreted as images, and circadian entrains a physiological clock. Here we describe local, non-visual response in mice that employs encephalopsin (OPN3, 480 nm, blue responsive opsin) to regulate function of adipocytes. Germ line null adipocyte-specific conditional show deficit thermogenesis is phenocopied under blue-light deficient...