A5062370631- Chromatin Remodeling and Cancer
- Renal and related cancers
- Cancer Mechanisms and Therapy
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- HER2/EGFR in Cancer Research
- CRISPR and Genetic Engineering
- interferon and immune responses
- Breast Cancer Treatment Studies
- Protein Degradation and Inhibitors
- Nanofabrication and Lithography Techniques
- Plant Genetic and Mutation Studies
- Biochemical and Molecular Research
- DNA Repair Mechanisms
- Transgenic Plants and Applications
- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Cell Image Analysis Techniques
- Cellular Mechanics and Interactions
- Polyamine Metabolism and Applications
Novartis (Switzerland)
2021-2024
Novartis Institutes for BioMedical Research
2021
Institut Curie
2018-2020
Inserm
2018-2020
Université Paris Sciences et Lettres
2020
Sorbonne Université
2018
Although fibroblast heterogeneity is recognized in primary tumors, both its characterization and impact on metastases remain unknown. Here, combining flow cytometry, immunohistochemistry RNA-sequencing breast cancer samples, we identify four Cancer-Associated Fibroblast (CAF) subpopulations metastatic lymph nodes (LN). Two myofibroblastic subsets, CAF-S1 CAF-S4, accumulate LN correlate with cell invasion. By developing functional assays cultures, demonstrate that these subsets promote...
A major challenge in cancer research is the complexity of tumor microenvironment, which includes host immunological setting. Inspired by emerging technology organ-on-chip, we achieved 3D co-cultures microfluidic devices (integrating four cell populations: cancer, immune, endothelial, and fibroblasts) to reconstitute ex vivo a human ecosystem (HER2+ breast cancer). We visualized quantified complex dynamics this tumor-on-chip, absence or presence drug trastuzumab (Herceptin), targeted antibody...
Abstract The Werner Syndrome RecQ helicase (WRN) is a synthetic lethal target of interest for the treatment cancers with microsatellite instability (MSI). Different hit finding approaches were initially tested. identification WRN inhibitors proved challenging due to high propensity artefacts via protein interference, i. e., hits inhibiting enzymatic activities through multiple, unspecific mechanisms. Previously published Helicase (ML216, NSC19630 or NSC617145) characterized in an extensive...
Abstract The transcription factor PAX8 is critical for the development of thyroid and urogenital system. Comprehensive genomic screens furthermore indicate an additional oncogenic role in renal ovarian cancers. While a plethora PAX8-regulated genes different contexts have been proposed, we still lack mechanistic understanding how engages molecular complexes to drive disease-relevant transcriptional programs. Here show that protein isoforms originating from MECOM locus form complex with PAX8....
Abstract ARID1B is a SWI/SNF subunit frequently mutated in human Coffin–Siris syndrome (CSS) and it necessary for proliferation of ARID1A mutant cancers. While most CSS aberrations introduce frameshifts or stop codons, the functional consequence missense mutations found unclear. We here perform saturated mutagenesis screens on demonstrate that protein destabilization main mechanism associated with pathogenic patients Syndrome.
Abstract Cell-cell interactions are an observable manifestation of underlying complex biological processes occurring in response to diversified biochemical stimuli. Recent experiments with microfluidic devices and live cell imaging show that it is possible characterize kinematics via computerized algorithms unravel the effects targeted therapies. We study influence spatial temporal resolutions time-lapse videos on motility interaction descriptors computational models mimic dynamics among...
Combination of functional epigenomics and single-cell CRISPR screens reveals that ER exerts its oncogenic role via downstream TFs.
The ARID1A and ARID1B subunits are mutually exclusive components of the BAF variant SWI/SNF chromatin remodeling complexes. Loss function mutations in frequently observed various cancers, resulting a dependency on paralog for cancer cell proliferation. However, has never been targeted directly, high degree sequence similarity to poses challenge development selective binders. In this study, we used mRNA display identify peptidic ligands that bind with nanomolar affinities showed selectivity...
A major challenge in cancer research is the complexity of tumor microenvironment and necessity to take into account host immunological setting. An innovative way address these problems exploit emerging technology organ-on-chip achieve co-cultures microfluidic devises that recapitulate ex vivo ecosystem.