A5051580994- SARS-CoV-2 and COVID-19 Research
- Intramuscular injections and effects
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Pharmaceutical studies and practices
- Heparin-Induced Thrombocytopenia and Thrombosis
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Cellular transport and secretion
- Long-Term Effects of COVID-19
- Herbal Medicine Research Studies
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Plant Reproductive Biology
- Olfactory and Sensory Function Studies
- Plant and Biological Electrophysiology Studies
- Phytoplasmas and Hemiptera pathogens
- Dermatology and Skin Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Biomedical and Engineering Education
- Photosynthetic Processes and Mechanisms
- COVID-19 epidemiological studies
- Biochemical and Structural Characterization
- Lipid Membrane Structure and Behavior
- COVID-19 and Mental Health
University of Cologne
2019-2022
University Hospital Cologne
2021-2022
Institute of Virology of the Slovak Academy of Sciences
2021
Bernhard Nocht Institute for Tropical Medicine
2021
University Medical Center Hamburg-Eppendorf
2021
Weatherford College
2021
Universität Hamburg
2021
Max Planck Institute for Plant Breeding Research
2008-2013
Sainsbury Laboratory
2010
Norwich Research Park
2010
N-Glycans attached to the ectodomains of plasma membrane pattern recognition receptors constitute likely initial contact sites between plant cells and invading pathogens. To assess role N-glycans in receptor-mediated immune responses, we investigated functionality Arabidopsis receptor kinases EFR FLS2, sensing bacterial translation elongation factor Tu (elf18) flagellin (flg22), respectively, N-glycosylation mutants. As revealed by binding responses elf18 or flg22, both tolerated immature...
The plant immune receptor FLAGELLIN SENSING 2 (FLS2) is present at the plasma membrane and internalized following activation of its ligand flagellin (flg22). We show that ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT (ESCRT)-I subunits play roles in FLS2 endocytosis Arabidopsis. VPS37-1 co-localizes with endosomes immunoprecipitates upon flg22 elicitation. Vps37-1 mutants are reduced flg22-induced but not labeled by Rab5 GTPases suggesting a defect trafficking rather than formation...
Highlights•AP2 μ2T156 phosphorylation starts early at CCP inception and increases as grows•Phosphorylation favors an AP2 conformational change also triggers NECAP binding•NECAP PHear simultaneously binds P-AP2 membrane-remodeling proteins on opposite faces•Disrupting the P-AP2:NECAP:membrane-remodeling protein network reduces CME ratesSummaryClathrin-mediated endocytosis (CME) is key to maintaining transmembrane composition of cells' limiting membranes. During mammalian CME, a reversible...
Abstract Membrane compartmentalization and trafficking within between cells is considered an essential cellular property of higher eukaryotes. We established a high-throughput imaging method suitable for the quantitative detection membrane compartments at subcellular resolution in intact epidermal tissue. Whole Arabidopsis (Arabidopsis thaliana) cotyledon leaves were subjected to confocal laser microscopy using automated image acquisition, computational pattern recognition, quantification...
Receptor-like kinases (RLKs) that function as pattern-recognition receptors (PRRs) play a key role in plant immune responses. The receptor recognizing flagellin Arabidopsis, FLS2, is encoded by membrane resident RLK. FLS2 involved preinvasive immunity against bacterial infection. Recent observations revealed upon perception accumulates intracellular mobile vesicles and then degraded. Reminiscent of ligand-induced endocytosis animals, internalization Wortmannin-sensitive. Mutation the...
Abstract A detailed understanding of antibody-based SARS-CoV-2 immunity has critical implications for overcoming the COVID-19 pandemic and informing on vaccination strategies. In this study, we evaluated dynamics antibody response in a cohort 963 recovered individuals over period 10 months. Investigating total 2,146 samples, detected an initial 94.4% individuals, with 82% 79% exhibiting serum IgG neutralization, respectively. Approximately 3% patients demonstrated exceptional neutralizing...
Abstract Background While the leading symptoms during coronavirus disease 2019 (COVID-19) are acute and majority of patients fully recover, a significant fraction now increasingly experience long-term health consequences. However, most data available focus on health-related events after severe infection hospitalization. We present longitudinal, prospective analysis consequences in who initially presented with no or minor respiratory syndrome type 2 (SARS-CoV-2) infection. Hence we mild...
(Developmental Cell 50, 494–508.e1–e11; August 19, 2019) As a result of an author oversight in the originally published version this article, Susanne Salomon was omitted from list. This error has now been corrected article online. The authors apologize for and any inconvenience that may have resulted. Temporal Ordering Endocytic Clathrin-Coated Vesicle Formation via AP2 PhosphorylationWrobel et al.Developmental CellAugust 2019In BriefWrobel al. show phosphorylation mammalian endocytic...
Abstract A detailed understanding of antibody-based SARS-CoV-2 immunity has critical implications for overcoming the COVID-19 pandemic and informing on vaccination strategies. In this study, we evaluated dynamics antibody response in a cohort 963 recovered individuals over period 10 months. Investigating total 2,146 samples, detected an initial 94.4% individuals, with 82% 79% exhibiting serum IgG neutralization, respectively. Approximately 3% patients demonstrated exceptional neutralizing...
A detailed understanding of antibody-based SARS-CoV-2 immunity has critical implications for overcoming the COVID-19 pandemic and informing vaccination strategies. We evaluated antibody response dynamics in a cohort 963 individuals over 10 months. Investigating 2,146 samples, we initially detected antibodies 94.4% individuals, with 82% 79% exhibiting serum IgG neutralization, respectively. Approximately 3% patients demonstrated exceptional SARS-CoV-2-neutralization, these 'elite...