A5005574178- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Advanced Fluorescence Microscopy Techniques
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Advanced Electron Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Chromosomal and Genetic Variations
Peking University
2019-2024
Abstract The genome exists as an organized, three-dimensional (3D) dynamic architecture, and each cell type has a unique 3D organization that determines its identity. An unresolved question is how type-specific structures are established during development. Here, we analyzed in muscle cells from mice lacking the lineage transcription factor (TF), MyoD, versus wild-type mice. We show MyoD functions “genome organizer” specifies architecture to development, H3K27ac insufficient for...
Clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic imaging systems predominantly rely on fluorescent protein reporters, which lack the optical properties essential for sensitive dynamic imaging. Here, we modified CRISPR single-guide RNA (sgRNA) to carry two distinct molecular beacons (MBs) that can undergo fluorescence resonance energy transfer (FRET) and demonstrated resulting system, CRISPR/dual-FRET MB, enables of non-repetitive loci with only three unique sgRNAs.
HIV-1 Gag proteins can multimerize upon the viral genomic RNA or multiple random cellular messenger RNAs to form a virus particle virus-like particle, respectively. To date, whether two types of particles via same multimerization process has remained unclarified. Using photoactivated localization microscopy illuminate organizations and dynamics at nanoscale, here, we showed that mediates in more cluster-centric, cooperative, spatiotemporally coordinated fashion, with ability drive dense...
Bimolecular Fluorescence Complementation (BiFC) is a versatile approach for intracellular analysis of protein-protein interactions (PPIs), but the tendency split fluorescent protein (FP) fragments to self-assemble when brought into close proximity each other by random collision can lead generation false-positive signals that hamper high-definition imaging PPIs occurring on nanoscopic level. While it thought expressing fusion proteins at low level remove false positives without impacting...
Abstract Many pathological processes are driven by RNA-protein interactions, making such interactions promising targets for molecular interventions. HIV-1 assembly is one process, in which the viral genomic RNA interacts with Gag protein and serves as a scaffold to drive multimerization that ultimately leads formation of virus particle. Here, we develop self-assembled nanostructures can inhibit assembly, achieved through hybridization multiple artificial small RNAs stem–loop structure (STL)...