A5101943995- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Fuel Cells and Related Materials
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Electrocatalysts for Energy Conversion
- Ammonia Synthesis and Nitrogen Reduction
- Molecular Biology Techniques and Applications
- Veterinary medicine and infectious diseases
- Cancer Genomics and Diagnostics
- Chromosomal and Genetic Variations
- Cardiac Ischemia and Reperfusion
- Quinazolinone synthesis and applications
- Advancements in Solid Oxide Fuel Cells
- Advanced Biosensing Techniques and Applications
- Genomics and Chromatin Dynamics
- Genomics and Rare Diseases
- Genomics and Phylogenetic Studies
- NF-κB Signaling Pathways
- Single-cell and spatial transcriptomics
Peking University
2017-2022
Yanbian University
2021
Shangqiu First People's Hospital
2021
Sun Yat-sen University
2008
Analysis of RNA dynamics and localization at the single-molecule level in living cells has been predominantly achieved by engineering target RNAs with large insertions tandem repeat sequences that are bound protein-based or oligonucleotide-based fluorescent probes. Thus, individual tagged multiple probes, making them detectable fluorescence microscopy. Since may affect processes including trafficking localization, here we present a strategy to visualize single transcripts using molecular...
Clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic imaging systems predominantly rely on fluorescent protein reporters, which lack the optical properties essential for sensitive dynamic imaging. Here, we modified CRISPR single-guide RNA (sgRNA) to carry two distinct molecular beacons (MBs) that can undergo fluorescence resonance energy transfer (FRET) and demonstrated resulting system, CRISPR/dual-FRET MB, enables of non-repetitive loci with only three unique sgRNAs.
The clustered regularly interspersed short palindromic repeat (CRISPR) gene-editing system has been repurposed for live-cell genomic imaging, but existing approaches rely on fluorescent protein reporters, making sensitive and continuous imaging difficult. Here, we present a fluorophore-based that consists of nuclease-deactivated mutant the Cas9 (dCas9), molecular beacon (MB), an engineered single-guide RNA (sgRNA) harboring unique MB target sequence (sgRNA-MTS), termed CRISPR/MB....
Abstract Background GPRIN1 may be a novel tumor regulator, but its role and mechanism in tumors are still unclear. Methods First, pan‐cancer correlation analysis was conducted on the expression prognosis of based data downloaded from The Cancer Genome Atlas (TCGA) database. Second, Starbase database used to predict upstream miRNAs lncRNAs GPRIN1, analysis, survival were performed screen microRNA (miRNAs)/long non‐coding RNAs (lncRNAs) that had with kidney renal papillary cell carcinoma...
MicroRNA (miRNA), which participates in various physiological and pathological processes, is a highly conserved small RNA sequence. This study aimed to investigate the role of miR-194-5p hypoxia/reoxygenation (H/R)-induced cardiomyocyte apoptosis myocardial ischemia/reperfusion (I/R) injury.We set up an H/R H9c2 cell model vitro I/R mouse vivo. Then, vitality, apoptosis, histopathological evaluation were conducted. Reactive oxygen species (ROS) generation activity superoxide dismutase (SOD)...
Abstract Molecular knowledge of human gastric corpus epithelium remains incomplete. Here, by integrated analyses using single-cell RNA sequencing (scRNA-seq), spatial transcriptomics and assay for transposase accessible chromatin (scATAC-seq) techniques, we uncovered the spatially-resolved expression landscape gene-regulatory network epithelium. Specifically, identified a stem/progenitor cell population in isthmus corpus, where EGF WNT signaling pathways were activated. Meanwhile, LGR4, but...
Abstract Nuclear Factor‐κB (NF‐κB) is an important transcription factor for regulation expression of many cytokines that are involved in the pathogenesis inflammatory diseases such as Adult Respiratory Distress Syndrome (ARDS), sepsis syndrome, and HIV. It recently proposed quinazoline derivatives capable inhibiting activation NF‐κB. To study mechanism obtain some helpful information designing functional inhibitor against protein, 3‐D Quantitative Structure–Activity Relationship (3D‐QSAR)...
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Modifying the electronic configuration is a key challenge but of prime importance to improve electrocatalytic performance. Incorporation doping heteroatoms could manipulate various chemical properties host materials, including accurate control wide variety catalytic reactions. This work establishes an anchored strategy in CoNi2S4 via introducing Mo-S bond accompanied by anion vacancy. As expected, oxygen reduction reaction (ORR) activity Mo (a-Mo-CoNi2S4) showed optimal performance with...
With the booming of next generation sequencing technology and its implementation in clinical practice life science research, need for faster more efficient data analysis methods becomes pressing field sequencing. Here we report on evaluation an optimized germline mutation calling pipeline, HummingBird, by assessing performance against widely accepted BWA-GATK pipeline. We found that HummingBird pipeline can significantly reduce running time primary whole genome exome while without...