A5107993762- interferon and immune responses
- Viral Infections and Vectors
- HIV Research and Treatment
- Inflammasome and immune disorders
- Epilepsy research and treatment
- Hemoglobin structure and function
- Glioma Diagnosis and Treatment
- Brain Metastases and Treatment
- Bacteriophages and microbial interactions
- Mosquito-borne diseases and control
- Chemical and Physical Studies
- Erythrocyte Function and Pathophysiology
- HIV/AIDS drug development and treatment
Washington University in St. Louis
2020-2024
John F. Kennedy Medical Center
2022
Illinois State University
2021
ABSTRACT A critical determinant for early post-entry events, the HIV-1 capsid (CA) protein forms conical core when it rearranges around dimeric RNA genome and associated viral proteins. Although mutations in CA have been reported to alter innate immune sensing of HIV-1, a direct link between stability nucleic acids has not established. Herein, we assessed how manipulating lattice through chemical genetic approaches affects recognition HIV-1. We found that destabilization resulted potent...
Abstract Two PIEZO mechanosensitive cation channels, PIEZO1 and PIEZO2, have been identified in mammals, where they are involved numerous sensory processes. While structurally similar, channels expressed distinct tissues exhibit unique properties. How different PIEZOs transduce force, how their transduction mechanism varies, properties match the functional needs of remain all-important unanswered questions. The nematode Caenorhabditis elegans has a single ortholog (pezo-1) predicted to 12...
Host factors that define the cellular tropism of SARS-CoV-2 beyond cognate ACE2 receptor are poorly defined. Here we report replication is restricted at a post-entry step in number ACE2-positive airway-derived cell lines due to tonic activation cGAS-STING pathway mediated by mitochondrial DNA leakage and naturally occurring cGAS STING variants. Genetic pharmacological inhibition type I/III IFN pathways as well overexpression overcome these blocks. knockout primary airway cultures induces ISG...
Seizures are a common occurrence. The goal of evaluating seizure is to identify the etiology and determine likelihood recurrence as well guide management. We present unique presentation 47-year-old female that presented with late onset seizures admitted due status epilepticus. Brain magnetic resonance indicated diffuse supratentorial hemorrhagic lesions. Neurological workup including brain vessel imaging, CT chest, abdomen, pelvis CSF serological for vasculitis failed demonstrate cause her...
ABSTRACT Host factors that define the cellular tropism of SARS-CoV-2 beyond cognate ACE2 receptor are poorly defined. Here we report replication is restricted at a post-entry step in number ACE2-positive airway-derived cell lines due to tonic activation cGAS-STING pathway mediated by mitochondrial DNA leakage and naturally occurring cGAS STING variants. Genetic pharmacological inhibition type I/III IFN pathways as well overexpression overcome these blocks. knockout primary airway cultures...
ABSTRACT The human immunodeficiency virus type 1 (HIV-1) capsid (CA) protein forms a conical lattice around the viral ribonucleoprotein complex (vRNP) consisting of dimeric genome and associated proteins, together constituting core. Upon entry into target cells, core undergoes process termed uncoating, during which CA molecules are shed from lattice. Although timing degree uncoating important for reverse transcription integration, molecular basis this phenomenon remains unclear. Using...