A5024976971- Stress Responses and Cortisol
- Neuroendocrine regulation and behavior
- Neuroscience and Neuropharmacology Research
- Extracellular vesicles in disease
- Neurogenesis and neuroplasticity mechanisms
- Epigenetics and DNA Methylation
- interferon and immune responses
- Birth, Development, and Health
- Lipid Membrane Structure and Behavior
- Neonatal and fetal brain pathology
- RNA regulation and disease
- Mitochondrial Function and Pathology
- Nerve injury and regeneration
- Cell Adhesion Molecules Research
- Sperm and Testicular Function
- Tryptophan and brain disorders
- Genetics and Neurodevelopmental Disorders
- Endoplasmic Reticulum Stress and Disease
- Neonatal Respiratory Health Research
- Retinal Development and Disorders
- Cellular Mechanics and Interactions
- Cancer, Stress, Anesthesia, and Immune Response
- Salivary Gland Disorders and Functions
- Developmental Biology and Gene Regulation
- Anesthesia and Neurotoxicity Research
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2024
National University of General San Martín
2013-2024
Instituto Tecnológico de Chascomús
2003-2018
Universidad Nacional de San Martín
2013
Research Centre in Biological Chemistry of Córdoba
1997-2004
Universidad Nacional de Córdoba
1997-2004
CIUDAD
1997
Prenatal stress (PS) exerts strong impact on fetal brain development and adult offspring functions. Previous work demonstrated that chronic alters the mRNA expression of GPM6A, a neuronal glycoprotein involved in filopodium extension. In this work, we analyzed effect PS gpm6a epigenetic mechanisms involved. Pregnant Wistar rats received restraint during last week gestation. Male were sacrificed postnatal days 28 60. Hippocampus prefrontal cortex samples for gene (qPCR mRNAs microRNAs),...
Several studies have demonstrated that the presence of stressors during pregnancy induces adverse effects on neuroendocrine system offspring later in life. In present work, we investigated early programming male reproductive system, employing a prenatal stress (PS) paradigm. This study found when pregnant dams were placed plastic restrainer three times day last week pregnancy, showed reduced anogenital distance and delayed testicular descent. Serum luteinising hormone (LH)...
Long-term exposure to stressful situations can affect the immune system. The T-cell response is an important component of anti-tumoral immunity. Hence, impairment function induced by a chronic stressor has been postulated alter immunosurveillance tumors, thus leading worse neoplastic prognosis. Here, we show that restraint stress affects mediated immunity in mice. This was evidenced decrease mitogen-induced proliferation, reduction CD4(+)T lymphocyte number and tumor necrosis factor-alpha...
Retinal ganglion cells send visual and circadian information to the brain regarding environmental light-dark cycles. We investigated capability of retinal synthesizing melatonin, a highly reliable marker that regulates physiology, as well capacity these function autonomous oscillators. Chick presented higher levels melatonin assessed by radioimmunoassay during both subjective day in constant darkness light phase cycle. Similar changes were observed mRNA activity arylalkylamine...
Prenatal stress (PS) strongly impacts fetal brain development and function in adulthood. In normal aging Alzheimer's disease, there is hypothalamic–pituitary–adrenal axis dysfunction loss of cholinergic neurons neuronal nicotinic acetylcholine receptors (nAChRs). This study investigated whether prenatal restraint affects nAChR expression the adult offspring. For PS, pregnant dams were placed a plastic restrainer for 45 min, three times daily during last week pregnancy; controls undisturbed....
M6a is a neuronal membrane glycoprotein whose expression diminishes during chronic stress. overexpression in rat primary hippocampal neurons induces the formation of filopodial protrusions that could be spine precursors. As filopodium and motility has been associated with synaptogenesis, we analysed M6a-induced by time-lapse imaging. Our data demonstrate motile formed overexpressing were more abundant moved faster than those control cells. When different putative phosphorylation sites...
Reproductive biotechnologies such as in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) enable improved reproductive efficiency of animals. However, the birth rate vitro-derived embryos still lags behind that their vivo counterparts. Thus, it is critical to develop an accurate evaluation prediction system embryo competence, both for commercial purposes scientific research. Previous works have demonstrated culture systems induce alterations relative abundance (RA) diverse...
Abstract Polysialyltransferases ST8SiaII/STX and ST8SiaIV/PST add polysialic acid (PSA) to the neural cell adhesion molecule (NCAM). Surface‐located PSA is involved in cell–cell interactions participating structural functional plasticity of neuronal circuits. This study was undertaken investigate polysialyltransferase regulation pattern during hippocampal development. Polysialyltransferase expression levels analyzed by real‐time RT‐PCR indicated that mRNA markedly down‐regulated vivo,...
Abstract Chronic stress causes morphological alterations in the hippocampus of rodents and tree shrews, including atrophy CA3 dendrites loss synapses. The molecular mechanisms underlying these structural changes remain largely unknown. We have previously identified M6a as a responsive gene shown that is involved filopodium/spine outgrowth and, likely, synapse formation. belongs to proteolipid protein (PLP) family, all their members having four transmembrane domains allow localization at...
We had previously shown that the transmembrane glycoprotein M6a, a member of proteolipid protein (PLP) family, regulates neurite/filopodium outgrowth, hence, M6a might be involved in neuronal remodeling and differentiation. In this work we focused on M6, only PLP family present Drosophila, ortholog to M6a. Unexpectedly, found decreased expression M6 leads female sterility. is expressed membrane follicular epithelium ovarioles throughout oogenesis. Phenotypes triggered by downregulation...
Abstract Two PIEZO mechanosensitive cation channels, PIEZO1 and PIEZO2, have been identified in mammals, where they are involved numerous sensory processes. While structurally similar, channels expressed distinct tissues exhibit unique properties. How different PIEZOs transduce force, how their transduction mechanism varies, properties match the functional needs of remain all-important unanswered questions. The nematode Caenorhabditis elegans has a single ortholog (pezo-1) predicted to 12...
Abstract Pathogens phagocytosis and the uptake of apoptotic cells (efferocytosis) are essential macrophages tasks, classically considered as mutually exclusive. Macrophages have been observed to polarize into either pro-inflammatory/microbicidal or anti-inflammatory/efferocytic phenotypes. However, macrophage functions shown be more complex. Furthermore, little is known about regulation efferocytosis under inflammatory conditions. In this study, we elucidate modulation efferocytic function...
The Developmental Origins of Health and Disease hypothesis sustains that exposure to different stressors during prenatal development prepares the offspring for challenges be encountered after birth. We studied gestational period as a particularly vulnerable window where can have strong implications fetal programming offspring's life-long metabolic status via alterations specific placentally expressed nutrient transporters. To study this mechanism, we used murine stress model, human...
Mental disorders affect millions of people worldwide. Their etiology is complex and the fact that main effects occur in brain hampers biochemical diagnosis. Therefore, biomarker finding peripheral fluids such as serum or saliva desirable. Here, we searched for biomarkers salivary extracellular vesicles (EVs). Then, focused on protein M6a, related to neuronal connectivity associated with several mood study its usefulness diagnosis depression anxiety. Biomarker candidates were by proteomic...
Members of the proteolipid protein family, including four-transmembrane glycoprotein M6a, are involved in neuronal plasticity mammals. Results from our group previously demonstrated that M6, only expressed Drosophila, localizes to cell membrane follicle cells. M6 loss triggers female sterility, which suggests a role for follicular remodeling. These results were basis present study, focused on function and requirements fly nervous system. The study identified two novel, tissue-regulated...
Abstract Membrane neuronal glycoprotein M6a is highly expressed in the brain and contributes to neural plasticity promoting neurite growth spine synapse formation. We have previously showed that chronic stressors alter hippocampal mRNA levels rodents tree shrews. now show can be detected mouse blood. a transmembrane and, as such, unlikely free Here we demonstrate that, blood, transported extracellular vesicles (EVs). It also shown M6a-containing EVs are delivered from cultured primary...
The nervous system monitors the environment to maintain homeostasis, which can be affected by stressful conditions. Using mammalian models of chronic stress, we previously observed altered brain levels GPM6A, a protein involved in neuronal morphology. However, GPM6A's role systemic stress responses remains unresolved. nematode Caenorhabditis elegans expresses GPM6A ortholog, membrane glycoprotein 1 (NMGP-1). Because shared features between and systems vast genetic tools available C. elegans,...