Metabolic functions are synchronized by the circadian clock setting daily patterns of food intake, nutrient delivery, and behavioral activity. Here, we study impact chronic jet-lag (CJL) on metabolism, test manipulations aimed to overcome potential alterations. We recorded weight gain in C57Bl/6 mice under 6 h advances or delays light-dark cycle every 2 days (ChrA ChrD, respectively). have previously reported ChrA, but not induce forced desynchronization locomotor activity rhythms (Casiraghi...

We have previously addressed that fructose rich diet (FRD) intake for three weeks increases the adipogenic potential of stromal vascular fraction cells from retroperitoneal adipose tissue (RPAT). now evaluated effect prolonged FRD (eight weeks) on metabolic parameters, number adipocyte precursor (APCs) and in vitro control (CTR) adult male rats. Additionally, we examined direct effects capacity normal APCs. fed rats had increased plasma levels insulin, triglyceride leptin, RPAT mass size....

An adverse endogenous environment during early life predisposes the organism to develop metabolic disorders. We evaluated impact of intake an iso-caloric fructose rich diet (FRD) by lactating mothers (LM) on several functions their male offspring. On postnatal d 1, ad libitum eating, Sprague-Dawley rats received either 10% F (wt/vol; FRD-LM) or tap water (controls, CTR-LM) drink throughout lactation. Weaned offspring were fed a normal diet, and body weight (BW) food registered until...

In the present study, we tested effect of OS (oxidative stress) inhibition in rats fed on an FRD [fructose-rich diet; 10% (w/v) drinking water] for 3 weeks. Normal adult male received a standard CD (commercial diet) or without with inhibitor NADPH oxidase, APO (apocynin; 5 mM water; CD-APO and FRD-APO). We thereafter measured plasma metabolic-endocrine markers, AAT (abdominal adipose tissue) mass cell size, FA (fatty acid) composition (content release), status, LEP (leptin) IRS (insulin...

Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little known about the relationship between white adipose tissue (WAT) thermogenesis. Therefore, aim of present study was evaluate this process. C57BL/6J male mice were treated or not with for ten days. On day 3, randomly divided into...

The intake of a fructose-rich diet (FRD) in the normal female rat induces features similar to those observed human metabolic syndrome phenotype. We studied impact FRD administration mothers on pregnancy outcome. On gestational day (Gd) zero rats were assigned either group: ad libitum drinking tap water alone (normal diet, ND) or containing fructose (10% w/vol; FRD) through pregnancy; all fed Purina chow ND and daily cotreated not with metformin (60 mg/Kg/day oral; + MF MF) submitted high...

Adipose tissue (AT) expansion is the result of two processes: hyperplasia and hypertrophy; both, directly or indirectly, depend on adipogenic potential adipocyte precursor cells (APCs). Glucocorticoids (GCs) have a potent stimulatory effect terminal adipogenesis; while their effects early stages adipogenesis are largely unknown. In present work, we study, in model high GC levels, APCs from retroperitoneal AT (RPAT) its relationship with RPAT mass expansion. We employed hyper-adiposity (30-...

Aim. To test the potential role of PPARγ in endocrine abdominal tissue dysfunction induced by feeding normal rats with a fructose rich diet (FRD) during three weeks. Methodology. Adult male received standard commercial (CD) or FRD, (10% drinking water) without pioglitazone (PIO) (i.p. 0.25 mg/Kg BW/day; CD-PIO and FRD-PIO). Thereafter, we measured circulating metabolic, endocrine, oxidative stress (OS) markers, adipose (AAT) mass, leptin (LEP) plasminogen activator inhibitor-1 (PAI-1)...

The aim of this work was to determine the effect a fructose rich diet (FRD) consumed by pregnant mother on endocrine-metabolic and in vivo vitro adipose tissue (AT) functions male offspring adulthood. At 60 days age, rats born FRD-fed mothers (F) showed impaired glucose tolerance after overload high circulating levels leptin (LEP). Despite diminished mass retroperitoneal AT, characterized enhanced LEP gene expression, hypertrophic adipocytes secreting larger amounts LEP. Analyses stromal...

Hyperandrogenemia predisposes an organism toward developing impaired insulin sensitivity. The aim of our study was to evaluate endocrine and metabolic effects during early allostasis induced by a fructose-rich diet (FRD) in normal (control; CT) neonatal-androgenized (testosterone propionate; TP) female adult rats. CT TP rats were fed either (ND) or FRD for 3 weeks immediately before the day study, which at age 100 days. Energy intake, body weight (BW), parametrial (PM) fat characteristics,...

The effect of progesterone (P4) on fructose rich diet (FRD) intake-induced metabolic, endocrine and parametrial adipose tissue (PMAT) dysfunctions was studied in the adult female rat. Sixty day-old rats were i.m. treated with oil alone (control, CT) or containing P4 (12 mg/kg). Rats ate Purina chow-diet ad libitum throughout entire experiment and, between 100 120 days age drank tap water (normal diet; CT-ND P4-ND) (10% w/v; CT-FRD P4-FRD). At days, animals subjected to a glucose tolerance...

Glucocorticoids (GCs) play a crucial role in energy homeostasis including white adipose tissue function; however, chronic GC excess is detrimental to mammals' health. White hypertrophic adiposity main factor for neuroendocrine-metabolic dysfunctions monosodium L-glutamate (MSG)-damaged hypercorticosteronemic rat. Nevertheless, little known about the receptor path endogenous impact on tissue-resident precursor cells bring them into beige lineage. Thus, our aim was explore whether...

An adverse endogenous environment during early life predisposes the organism to develop metabolic disorders. We evaluated impact of intake an iso-caloric fructose rich diet (FRD) by lactating mothers (LM) on several functions their male offspring. On postnatal d 1, ad libitum eating, Sprague-Dawley rats received either 10% F (wt/vol; FRD-LM) or tap water (controls, CTR-LM) drink throughout lactation. Weaned offspring were fed a normal diet, and body weight (BW) food registered until...

White adipose tissue (WAT) regulates energy balance through storage, adipokines secretion and the thermogenesis process. Beige adipocytes are responsible for WAT thermogenesis. They generated by adipogenesis or transdifferentiation during cold β3-adrenergic agonist stimulus a process called browning. Browning has gained significant interest to its preventive effect on obesity. Glucocorticoids (GCs) have several functions in biology; however, their role beige adipocyte generation browning is...

study, we investigated the role of ␣CGRP in lipid metabolism by chronically challenging ␣CGRP-specific knockout (␣CGRP Ϫ/Ϫ ) and control mice with high-fat diet regimens.At start both animal groups displayed similar body weights, serum markers, insulin sensitivity.However, higher core temperatures, increased energy expenditures, a relative daytime (nonactive) depression respiratory quotients, which indicated ␤-oxidation.In response to fat feeding, were comparatively protected against...