ABSTRACT Lettuce downy mildew ( Bremia lactucae ) is one of the most important diseases lettuce, especially in conditions mild temperatures and high humidity. The great variability B . makes use genetic control complex, as resistance to different virulence phenotypes requires factors. Dm (“Downy mildew”) genes or factors, have been widely used lettuce cultivars, providing a level mildew. objective this work was monitor study south southeast regions Brazil. Samples with symptoms were...

Leaf spot and crown rot caused by Neopestalotiopsis spp. are relevant emerging diseases of strawberries worldwide. This study aimed to: i) identify characterize isolates recovered from different strawberry plant organs; ii) determine the pathogenic variation those isolates; iii) response 13 varieties against N. rosae. Four hundred ninety-eight were obtained leaves, roots crowns in central Mexico 2020 to 2021. A subsample 160 was selected for characterization using a previously developed...

Alocasia ‘Calidora’ (NCSU, 2025), known as upright elephant ear, is an ornamental plant grown for its large, glossy leaves and architectural value. In September 2023, diseased 'Calidora' plants were submitted by a commercial grower from Highlands County, FL, to the Diagnostic Clinic at University of Florida’s Gulf Coast Research Education Center. The reported about 10% disease incidence in field 3 ha with estimated 30,000 plants. plants, initially propagated tissue-cultured starters...

Annual strawberry production in Florida is threatened by anthracnose, caused Colletotrichum acutatum. This disease often transmitted through quiescently infected transplants from nurseries. Managing anthracnose has become even more difficult both nursery and fruit fields since the rise of strains resistant to QoI fungicides. In this study, thermotherapy was evaluated as a potential alternative manage reducing inoculum plant stock. Initial laboratory assessments demonstrated that germination...

Phytophthora cactorum and nicotianae cause leather rot on fruit crown (PhCR) of strawberry plants. Leather is not a common disease in Florida; however, up to 50% yield loss has been reported harvests after intense rainfall events. PhCR an important worldwide characterized by sudden wilting collapse Mefenoxam the most effective widely used fungicide control both diseases. P. isolates from have collected multiple fields Florida since 1997, sensitivity 185 was tested at 0, 0.05, 0.5, 5, 100...

Phytophthora crown rot, caused mainly by cactorum but also P. nicotianae, reported in 2018, is an important disease the Florida strawberry annual production system. Mefenoxam most effective and widely used fungicide to manage this disease. However, because of pathogen resistance, alternatives chemical control are needed. spp. were rarely recovered during summer from soil commercial farms where was observed season. In a more detailed survey on research plots, neither two species 1 month after...

The okra (Abelmoschus esculentus L. Moench) is one of the most important vegetables in world and a popular food item many tropical subtropical countries. Besides its cultivation for fresh consumption, also has potential industrial uses. Nonetheless, pests diseases remain damaging factors affecting crop yield. Among these, root-knot nematodes are main limiting production. This study aimed to determine responses plant species Malvaceae family Meloidogyne incognita M. enterolobii nematodes,...

Phytophthora crown rot (PhCR) is an important disease of strawberry worldwide. cactorum the most common causal agent, however, P. nicotianae was also recently reported causing PhCR in U.S. Therefore, goals this study were to evaluate resistance cultivars from Florida and California, etiology two species PhCR. Sixteen evaluated over three seasons for susceptibility cactorum, nicotianae. Inoculations at different days after transplanting (DAT) carried out ability both cause phenological stages...

Phytophthora cactorum and P. nicotianae cause leather rot (LR) of fruit crown (PhCR) in strawberry. LR occurs sporadically but can up to 70% loss when weather is conducive. In Florida's annual strawberry winter production system, PhCR be severe, resulting plant stunting, mortality, severe yield loss. Azoxystrobin labeled for control not PhCR. The aims this research were determine the sensitivity isolates from azoxystrobin investigate mechanisms quinone-outside-inhibitor resistance present...

ABSTRACT Genetic resistance is one of the most suitable strategies to control cucurbit powdery mildew (CPM) on melon, incited by Podosphaera xanthii or Golovinomyces orontii. However, many races these pathogens have been reported worldwide in recent years, what may compromise effectiveness this method. Thus, annual surveys CPM and screening germplasm for new sources genetic provide a vital support melon breeding programs. This study aimed at identifying natural population race under...

Florida strawberry production is affected by two economically important Phytophthora diseases, crown rot (PhCR) and leather (LR), caused primarily cactorum P. nicotianae. Although products are available, chemical control limited to mefenoxam, phosphites, azoxystrobin, resistance mefenoxam azoxystrobin has been reported. Both in vitro vivo assays were conducted evaluate the effectiveness of eight different classes on controlling PhCR LR strawberry. The fungicides mandipropamid, fluopicolide,...

Phytophthora crown rot (PhCR) caused by cactorum is one of the most damaging soilborne diseases strawberry in United States and worldwide. Limitations on fumigants such as methyl bromide have led to deterioration control P . recent years. The development resistant varieties a critical component strategy for combatting this disease. Here, we review biology pathogen, molecular mechanisms infection, management PhCR strawberry. Recent genomics advances octoploid breeding been able gain deeper...

A new Neopestalotiopsis sp. was recently reported causing outbreaks of leaf spot and fruit rot on strawberry in Florida, Georgia, South Carolina. In contrast to other Pestalotiopsis pathogens, the species appears more aggressive destructive strawberry. Current chemical options for management are disease suppressive at best, affected growers have been experiencing major yield losses. this study, we developed a molecular method based polymerase chain reaction/restriction fragment length...

Recently, the Florida strawberry industry faced unprecedented outbreaks of an emerging disease caused by fungus Neopestalotipsis spp. Currently, there are no fungicides labeled to control this in United States and efficacy single- multisite is unknown. Therefore, study aimed determine vitro sensitivity Neopestalotiopsis isolates with different modes action evaluate these products on detached fruit field. In preliminary tests, 30 commercially available were screened using discriminatory...

Rapid and accurate disease diagnosis is a prerequisite for an effective management program in strawberry production. In Florida, Colletotrichum spp., Phytophthora Macrophomina phaseolina are the primary microorganisms causing crown rot. Even though diseases can be caused by different pathogens, symptoms indistinguishable equally devastating. To inform growers timely fashion of diagnostic results deployment chemical control practices, we developed multiplex high-resolution melting (HRM) assay...

Caladium (Caladium × hortulanum) is an ornamental plant popular for its variable and colorful foliage. In 2020, plants showing leaf spots blight, typical of anthracnose, were found in a field trial at the University Florida’s Gulf Coast Research Education Center Wimauma, Florida, U.S.A. Leaf samples consistently yielded Colletotrichum-like species with curved conidia abundant setae production acervuli. The internal transcribed spacer (ITS), partial sequences glyceraldehyde-3-phosphate...

During the 2019-2020 Florida strawberry season (October to April), a (

Since 2017, a new Neopestalotiopsis sp. has been reported causing severe Pestalotia leaf spot (PLS) outbreaks in Florida. The threat of this species forced several strawberry growers to destroy their fields early the season. need for fast and accurate diagnosis PLS came from growers’ concerns fact that symptoms can be easily confused with spots caused by other pathogens. Moreover, although N. rosae isolated symptomatic tissues, it is not as aggressive Therefore, study aimed develop...

In underdeveloped countries, zinc deficiency is a public health problem. The main foods consumed in these countries have low levels of the nutrient, making their consumption insufficient to meet minimum daily requirements. Therefore, aim this study was evaluate agronomic biofortification with curly lettuce cultivars. experiment carried out at Universidade Federal de Uberlândia - Monte Carmelo Campus, using complete randomized block design 4 × 5 factorial scheme four replications. factors...