J Laurent

ORCID: 0000-0002-5198-1326
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About
Contact & Profiles
Research Areas
  • Hungarian Social, Economic and Educational Studies
  • Innovative Microfluidic and Catalytic Techniques Innovation
  • Hydrogen embrittlement and corrosion behaviors in metals
  • Legume Nitrogen Fixing Symbiosis
  • Fusion materials and technologies
  • Electrospun Nanofibers in Biomedical Applications
  • Mass Spectrometry Techniques and Applications
  • Polysaccharides and Plant Cell Walls
  • Advanced Materials Characterization Techniques
  • biodegradable polymer synthesis and properties
  • 3D Printing in Biomedical Research
  • Electrodeposition and Electroless Coatings
  • Banana Cultivation and Research
  • Glycosylation and Glycoproteins Research
  • Multiculturalism, Politics, Migration, Gender
  • Microbial Community Ecology and Physiology
  • Non-Destructive Testing Techniques
  • Tissue Engineering and Regenerative Medicine
  • Microfluidic and Capillary Electrophoresis Applications
  • Microbial Metabolic Engineering and Bioproduction
  • Political and Social Issues
  • Plant Pathogenic Bacteria Studies
  • Gene Regulatory Network Analysis
  • CRISPR and Genetic Engineering
  • Anodic Oxide Films and Nanostructures

ETH Zurich
2023-2024

École Polytechnique Fédérale de Lausanne
1985-2021

Massachusetts Institute of Technology
2021

University of Lausanne
2009

University Hospital of Lausanne
2009

Assemblée Nationale de France
2004

University of Amsterdam
1993

Nature is home to a variety of microorganisms that create materials under environmentally friendly conditions. While this offers an attractive approach for sustainable manufacturing, the production by native usually slow and synthetic biology tools engineer faster are only available when prior knowledge genotype-phenotype links available. Here, we utilize high-throughput directed evolution platform enhance fitness whole selection pressure identify genetic pathways material capabilities species. Using

10.1073/pnas.2403585121 article EN cc-by Proceedings of the National Academy of Sciences 2024-07-23

Supernatants from cell cultures (also called conditioned media, CMs) are commonly analyzed to study the pool of secreted proteins (secretome). To reduce exogenous protein background, serum-free media often used obtain CMs. Serum deprivation, however, can severely affect viability and phenotype, including secretion. We present a strategy analyze by cells in fetal bovine serum-containing CMs, which combines advantage metabolic labeling concentration linearization techniques. Incubation CMs...

10.1021/pr900476b article EN Journal of Proteome Research 2009-08-27

Esophageal diseases may require resectioning of the damaged portion. The current standard care requires replacement esophagus with stomach or intestine. Such procedures have high rates mortality and morbidity; therefore, use alternative conduits is needed. A tissue engineering approach that allows for regeneration esophageal tissues would significant clinical application. cell-seeded synthetic scaffold could replace resected part elicit regrowth. In order to ideally recreate a functioning...

10.1002/jbm.b.34124 article EN Journal of Biomedical Materials Research Part B Applied Biomaterials 2018-05-02

10.4028/www.scientific.net/msf.44-45.213 article EN Materials science forum 1991-01-01

Microorganisms can create engineered materials with exquisite structures and living functionalities. Although synthetic biology tools to genetically manipulate microorganisms continue expand, the bottom-up rational design of still relies on prior knowledge genotype-phenotype links for function interest. Here, we utilize a high-throughput directed evolution platform enhance fitness whole under selection pressure identify novel genetic pathways program functionalities materials. Using...

10.48550/arxiv.2311.13342 preprint EN cc-by-nc-nd arXiv (Cornell University) 2023-01-01

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10.1017/s0269888900004252 article EN The Knowledge Engineering Review 1988-06-01

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10.1017/s0269888900005002 article EN The Knowledge Engineering Review 1989-09-01

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10.1017/s0269888900004707 article EN The Knowledge Engineering Review 1989-03-01

Abstract Added at equal atomic concentration (6 at.%) to ferritic Fe‐ 13 Cr alloys, M0, W, Vand Si increase the pitting potential in 1 M HCl order Mo V W. Addition of same elements an Fe‐24 alloy suppresses entirely.

10.1002/chin.198613018 article EN Chemischer Informationsdienst 1986-04-01
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