A5065037104- Autophagy in Disease and Therapy
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- Mitochondrial Function and Pathology
- Politics and Conflicts in Afghanistan, Pakistan, and Middle East
- Hepatitis B Virus Studies
- Liver Disease and Transplantation
- Online and Blended Learning
- Higher Education Governance and Development
- Parkinson's Disease Mechanisms and Treatments
- Toxin Mechanisms and Immunotoxins
- Lysosomal Storage Disorders Research
- Poverty, Education, and Child Welfare
- Gastrointestinal Bleeding Diagnosis and Treatment
- Pancreatitis Pathology and Treatment
- Early Childhood Education and Development
- Doctoral Education Challenges and Solutions
- Pancreatic and Hepatic Oncology Research
- Microtubule and mitosis dynamics
- Peace and Human Rights Education
- Botanical Research and Chemistry
- Mechanical Engineering Research and Applications
- Educational Technology and Optimization
- Morinda citrifolia extract uses
Structural Genomics Consortium
2024
McGill University
2015-2024
Post Graduate Medical Institute
2016-2024
Government College University, Faisalabad
2015-2024
Lahore General Hospital
2019-2024
Army Medical College
2024
University of Northampton
2023
Minhaj University Lahore
2022
Islamia University of Bahawalpur
2015-2022
Veterinary & Animal Husbandry
2017
Abstract Mutations in Parkin and PINK 1 cause an inherited early‐onset form of Parkinson's disease. The two proteins function together a mitochondrial quality control pathway whereby accumulates on damaged mitochondria activates to induce mitophagy. How kinase activity releases the auto‐inhibited ubiquitin ligase remains unclear. Here, we identify binding switch between phospho‐ubiquitin ( pU b) ubiquitin‐like domain (Ubl) as key element. By mutagenesis SAXS , show that b binds RING at site...
Abstract We present the nELISA, a high-throughput, high-fidelity, and high-plex protein profiling platform. DNA oligonucleotides are used to pre-assemble antibody pairs on spectrally encoded microparticles perform displacement-mediated detection. Spatial separation between non-cognate antibodies prevents rise of reagent-driven cross-reactivity, while read-out is performed cost-efficiently at high-throughput using flow cytometry. assembled an inflammatory panel 191 targets that were...
This article deals with the laminar flow of a viscous fluid and heat transfer analysis due to porous stretching/shrinking cylinder partial slip condition. The equations corresponding momentum energy are transformed into set highly nonlinear ordinary differential by means similarity transformations, which then, solved numerically using Runge–Kutta–Fehlberg method. physical significance various involved parameters on velocity temperature distribution is discussed through graphs tables in...
This study assesses the transformative impact of STEM-based training on students’ critical thinking skills and attitudes toward STEM disciplines, while also evaluating its effectiveness with conventional techniques. Using an effective Solomon Four-Group Experimental Design involving 8th grade students, findings show that STEM-integrated education has significant benefits in developing analytical reasoning, problem solving, deep interest scientific research. Traditional teaching methods,...
Oculocutaneous albinism (OCA) is caused by a group of genetically heterogeneous inherited defects that result in the loss pigmentation eyes, skin and hair. Mutations TYR, OCA2, TYRP1 SLC45A2 genes have been shown to cause isolated OCA. No comprehensive analysis has conducted study spectrum OCA alleles prevailing Pakistani albino populations.We enrolled 40 large families screened them for candidate gene, SLC24A5. Protein function effects were evaluated using silico prediction algorithms ex...
Mutations in Parkin and PINK1 cause early-onset familial Parkinson's disease. is a RING-In-Between-RING E3 ligase that transfers ubiquitin from an E2 enzyme to substrate two steps: (i) thioester intermediate formation on (ii) acyl transfer lysine. The process triggered by PINK1, which phosphorylates damaged mitochondria, turn recruits activates Parkin. This leads the ubiquitination of outer mitochondrial membrane proteins clearance organelle. While targets mitochondria are known, factors...
Abstract Mitochondria dysfunction is involved in the pathomechanism of many illnesses including Parkinson’s disease. PINK1, which mutated some cases familial Parkinsonism, a key component degradation damaged mitochondria by mitophagy. The accumulation PINK1 on mitochondrial outer membrane (MOM) compromised organelles crucial for induction mitophagy, but molecular mechanism this process still unresolved. Here, we investigate association with TOM complex. We demonstrate that heavily relies...
The purpose of current study was to explore the effect digital literacy on academic performance students at higher education level. mixed method and data were gathered with a questionnaire semistructured interviews. validity reliability scales ensured through experts' opinion, pilot testing Croanbach Alpha score. population comprised M.S/M.Phil Ph.D. A sample 800 selected randomly from 10 Universities. statistical tests like mean, standard deviation correlation used. Results revealed that...
Abstract Mutations in PINK1 and Parkin cause early-onset Parkinson’s Disease (PD). is a kinase which functions as mitochondrial damage sensor initiates quality control by accumulating on the damaged organelle. There, it phosphorylates ubiquitin, turn recruits activates Parkin, an E3 ubiquitin ligase. Ubiquitylation of proteins leads to autophagic degradation Pharmacological modulation constitutes appealing avenue study its physiological function develop therapeutics. In this study, we used...
Abstract Background There are conflicting data regarding the risk of hepatocellular carcinoma (HCC) after direct-acting antiviral agent (DAA) treatment. Risk HCC in HCV genotype-3 infected persons DAA therapy is not well known. Methods We prospectively studied initiated on a regimen between October 2014 and March 2017 at two centers Pakistan. All were free study initiation. was confirmed based characteristic CT scan findings. Patients followed for 12 months completion therapy. Results A...
Heads are leading figures in educational institutions responsible for the training of future generations but also have to perform many key functions achieve predetermined objectives. The objective was a comparison management effectiveness between direct selectees and promoted secondary school headteachers Punjab. A close-ended rating scale consisting 25 statements developed on five-point Likert 464 senior Secondary School Teachers (SSTs) selected through multistage random sampling. Nine...
Switching of bacterial flagellar rotation is caused by large domain movements the FliG protein triggered binding signal CheY to FliM. and FliM form adjacent multi-subunit arrays within basal body C-ring. The alter interaction C-terminal (FliGC) “torque” helix with stator complexes. Atomic models based on Salmonella entrovar C-ring electron microscopy reconstruction have implications for switching, but lack consensus relative locations armadillo (ARM) domains (amino-terminal (FliGN), middle...
<p class="apa">The rationalization of this research was to investigate about improving professional development system through Quality Assurance Practices (QAP) in the Universities Pakistan pertaining opinions students, teachers and Directors Enhancement Cells’ (QEC’s) differentiate ideas QECs regarding as well offer quality assurance practices universities Pakistan. This study had a quantitative qualitative design. conducted on sample 28 (public private sector) by using random...
Fatigue failure remains a critical concern in structural engineering and material science, prompting extensive research to understand predict the behaviour of materials under cyclic loading conditions. The present study aims investigate fatigue life carbon steel specimens containing opposite semicircular edge notches through comprehensive experimental numerical analysis. In this study, stress concentration factors (SCF, Kt) rectangular plate with are considered uniform tensile analyse notch...
The current study reports occurrence and molecular toxinotyping of Clostridium perfringens isolates in Kashmir valley. A total 217 feacal samples intestinal contents broiler chickens suspected for enteritis were collected screened C. out which 66 (30.41%) found positive using Robertson’s cooked meat media Sulphite Polymixin Sulphadiazine (SPS) agar. confirmed by 16S rRNA gene specific PCR analyzed multiplex toxinotyping. All the to harbour alpha toxin (cpa) perfringens. However, 13 also...
This study employs a qualitative approach to get insights into the experiences of research students during COVID-19 pandemic and their perspectives on government higher education institutions. A total 50 from three universities were interviewed utilising inductive thematic method. The engaged in discourse regarding dissemination misinformation social media, hazardous behaviour, need taking preventive measures crisis. They cognizant hazards associated with nursing profession as well...
Peptic ulcers are a global health concern. Bleeding episodes, common complication require urgent intervention. Endoscopic therapy for managing bleeding peptic offering both diagnostic and therapeutic benefits. Objective: To analyze the characteristics of assess outcomes following endoscopic therapy. Methods: A descriptive case series was conducted at Lahore General Hospital (LGH) between June 2021 January 2022. Patients aged 18-80 with upper GI due to or undergoing endoscopy were included....
This study aims to explore the attitudes of undergraduate students majoring in social sciences at University Sargodha towards assessment for learning. It recognizes substantial impact that methods have on students' habits and academic performance. A cohort 50 from Education Psychology departments B.S. 8th level were involved research. The investigated motivation psychological factors associated with classroom management approaches using a self-designed questionnaire required participants...
An apple twig shredder was developed to manage waste and produce substrate for shiitake mushroom cultivation, mulch, media clonal propagation of root stocks. The machine evaluated power requirement, throughput capacity, shredding efficiency, size shredded material. standardized at 2880 rpm rotational speed 15-25 mm diameter which efficiency material obtained as 1.06 kW, 28.24 kgh-1, 82.50 % 0.735 respectively. Operational cost estimated 70.38 INRh-1 2.5 INRh-1.
Endoscopic ultrasound guided fine needle aspiration and biopsy (EUS-FNA/FNB) are minimally invasive yet very effective techniques for tissue acquisition diagnosis of sub-epithelial or other lesions in close premises gastrointestinal tract. Objective: To evaluate the diagnostic accuracy safety EUS-FNA FNA/FNB various lesions. Methods: This was a single center study consecutive 189 patients who presented to Gastroenterology department Lahore General Hospital, EUS during October 2019 March...