Despite the economic importance of sugarcane in sugar and bioenergy production, there is not yet a reference genome available. Most transcriptomic studies have been based on Saccharum officinarum gene indices (SoGI), expressed sequence tags (ESTs) de novo assembled transcript contigs from short-reads; hence knowledge transcriptome limited relation to length number isoforms.The was sequenced using PacBio isoform sequencing (Iso-Seq) pooled RNA sample derived leaf, internode root tissues,...

The senescence of fruit is a complex physiological process, with various cell types within the pericarp, making it highly challenging to elucidate their individual roles in senescence. In this study, single-cell expression atlas pericarp pitaya (Hylocereus undatus) constructed, revealing exocarp and mesocarp cells undergoing most significant changes during process. Pseudotime analysis establishes cellular differentiation gene trajectories Early-stage oxidative stress imbalance followed by...

Recent advances in genome sequencing and assembly techniques have made it possible to achieve chromosome level reference genomes for citrus. Relatively few been anchored at the and/or are haplotype phased, with available of varying accuracy completeness. We now report a phased high-quality an Australian native citrus species;

Sugarcane accumulates very high levels of sucrose in the culm. Elucidation molecular mechanisms that allows such synthesis and accumulation (up to 650 mM) is made difficult by complexity highly polyploid genome. Here we report use RNA Seq data characterize synthase (SuSy) genes expressed transcriptome mature sugarcane plant.Four SuSy gene families were identified Iso-Seq long read (SUGIT) through annotation transcripts mapped reference from sorghum maize. In total, 38, 19, 14, 2 for four...

Although many schools are presenting acquired immunodeficiency syndrome (AIDS) education programs for adolescents, few have evaluated the effects of programs. The two different types program presentation, a lecture or film, were compared to no-program condition. Students who received demonstrated significantly greater knowledge gains than either other groups. group's gain was maintained at 1-month follow-up, although all three groups showed decline in scores from posttest follow-up. Both...

The importance of uridine 5'-diphosphate glucose (UDP-G) synthesis and degradation on carbon (C) partitioning has been indicated in several studies plant systems, whereby the kinetic properties abundance involved enzymes had a significant effect upon volume C moving into hemicellulose, cellulose sucrose pools. In this study, expression 136 genes belonging to 32 gene families related UDP-G metabolism was studied 3 major sugarcane organs (including leaf, internode root) at 6 different...

Plant breeders leverage mutagenesis using chemical, biological, and physical mutagens to create novel trait variations. Many widely used sorghum genotypes have a narrow genetic base, which hinders improvements classical breeding. Enhancing the diversity of genome thus remains key priority for breeders. To accelerate enhancement sorghum, an extensive library comprised seeds from 150,000 individual mutant plants Sorghum bicolor inbred line BTx623 was established ethyl methanesulphonate (EMS)...

The purpose of this program was to evaluate the benefits integrating VA Care Coordination Home Telehealth and Telemental health within HBPC. A case study design used determine quality assurance improvement incorporating additional home telehealth equipment Based Primary (HBPC). Veterans with complex medical conditions their caregivers living in rural Oklahoma were enrolled. received same care other HBPC patients addition equipment. Members from interdisciplinary treatment team certified use...

Journal Article Psychological Consultation in a Children's Hospital: An Evaluation of Services Get access Roberta A. Olson, Olson 1 University Oklahoma Health Sciences Center 1All correspondence should be addressed to Department Psychiatry and Behavioral Sciences, Center, P.O. Box 26901, City, 73190 Search for other works by this author on: Oxford Academic PubMed Google Scholar E. Wayne Holden, Holden Auburn Alice Friedman, Friedman St. Jude's Hospital Jan Faust, Faust Stanford Mary Kenning,...

Standards of cleft care abroad differ from those in the United States, particularly less developed countries, where international adoption rates are high. Children adopted these countries present to plastic surgeons States at various ages and states repair. The operative perioperative needs children poorly understood. This study attempts characterize preadoption history, postadoption course, surgical outcomes with deformities.The authors performed a retrospective review all lip-cleft palate...

Abstract Background The composition of biomass determines its suitability for different applications within a biorefinery system. proportion the major fractions (sugar, cellulose, hemicellulose and lignin) may vary in sugarcane genotypes growth environments parts plant. This study investigated mature immature internodes, roots leaves sugarcane. Results Internodes were found to have significantly larger alcohol-soluble component than roots. primary difference between internodes was ratio...

The purpose of this study was to determine the efficacy and toxicity a doxorubicin/cyclophosphamide-based chemotherapy local radiation therapy in children with locally advanced or metastatic nasopharyngeal carcinoma (NPC).Twelve patients aged 6 20 years old were treated regimen comprised vincristine (1.5 mg/m2) doxorubicin (45 on day 1 cyclophosphamide (210 5-fluorouracil (240 days 5. Chemotherapy administered every 3 weeks for 2 years. Radiotherapy primary site (59 68 Gy) neck 66 given...

The major carbon source and sink organs (roots, leaves internodes) of sugarcane (Saccharum spp. hybrids) have different roles consequently apportioning into the components these organs. This study performed RNA-seq on two leaves, three internodes (of differing maturity) roots in similar commercial genotypes to determine patterns gene expression associated with differences partitioning. A total 18 libraries (in triplicate) were generated per genotype, thus yielding ∼0.6 billion high quality...

Informational needs of hemophiliacs must first be assessed to develop effective educational and prevention programs. A survey 132 hemophilia patients family members was conducted determine the information needs, preferred source patients' knowledge AIDS. Results indicated that major for media; treatment center. Most wanted more about AIDS how cope with stresses Although they had received sufficient sexual transmission AIDS, answers questions important misconceptions. are discussed respect...

Abstract The genus Macadamia in the Proteaceae family includes four species. To explore diversity macadamia genetic resources, a total of 166 wild genotypes, representing all species, were sequenced. species clearly distinguished as separate clades phylogenetic analysis nuclear gene CDS. M. integrifolia and tetraphylla formed clade that had diverged from including ternifolia jansenii. greatest chloroplast genomes was found more widely distributed while rare jansenii showed little diversity....

<title>Abstract</title> Australian wild limes occur in highly diverse range of environments and are a unique genetic resource within the genus <italic>Citrus</italic>. Here we compare haplotype-resolved genome assemblies six native limes, including four new generated using PacBio HiFi Hi-C sequencing data. The size genomes was between 315 391 Mb with contig N50s from 29.5 to 35 Mb. Gene completeness estimated be 98.4–99.3% annotations 97.7–98.9% based upon BUSCO, confirming high contiguity...

Abstract Australian wild limes occur in highly diverse range of environments and are a unique genetic resource within the genus Citrus . Here we compare haplotype-resolved genome assemblies six native limes, including four new generated using PacBio HiFi Hi-C sequencing data. The size genomes was between 315 391 Mb with contig N50s from 29.5 to 35 Mb. Gene completeness estimated be 98.4 99.3% annotations 97.7 98.9% based upon BUSCO, confirming high contiguity assembled genomes. High...

The genus Macadamia in the Proteaceae family includes four species native to Australia. Two of species, M. integrifolia and tetraphylla , have recently been utilized generate domesticated macadamia varieties, grown for their edible nuts. To explore diversity genetic resources, a total 166 wild genotypes, representing all were sequenced. clearly distinguished as separate clades phylogenetic analysis nuclear genome (based upon concatenated gene CDS SNPs). two larger ( ) formed clade, that had...