A5032616219- Alzheimer's disease research and treatments
- Tryptophan and brain disorders
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA regulation and disease
- COVID-19 Clinical Research Studies
- Bioinformatics and Genomic Networks
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- RNA Research and Splicing
- Machine Learning in Bioinformatics
- Ubiquitin and proteasome pathways
- COVID-19 epidemiological studies
- interferon and immune responses
- Glycosylation and Glycoproteins Research
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
- Peroxisome Proliferator-Activated Receptors
- Salmonella and Campylobacter epidemiology
- COVID-19 and Mental Health
- Healthcare Systems and Reforms
- COVID-19 and healthcare impacts
- Immune Response and Inflammation
- Academic Publishing and Open Access
WuXi AppTec (China)
2021-2024
Soochow University
2024
Emory University
2019-2021
Wuhan University
2014-2016
State Key Laboratory of Virology
2015
Institute of Virology of the Slovak Academy of Sciences
2015
Wuhan Institute of Virology
2014
Alzheimer's disease (AD) lacks protein biomarkers reflective of its diverse underlying pathophysiology, hindering diagnostic and therapeutic advancements. Here, we used integrative proteomics to identify cerebrospinal fluid (CSF) representing a wide spectrum AD pathophysiology. Multiplex mass spectrometry identified ~3500 ~12,000 proteins in CSF brain, respectively. Network analysis the brain proteome resolved 44 biologically modules, 15 which overlapped with proteome. markers these...
Viral infection triggers induction of type I interferons (IFNs), which are critical mediators innate antiviral immune response. Mediator IRF3 activation (MITA, also called STING) is an adapter essential for virus-triggered IFN pathways. How post-translational modifications regulate the activity MITA not fully elucidated. In expression screens, we identified RING finger protein 26 (RNF26), E3 ubiquitin ligase, could mediate polyubiquitination MITA. Interestingly, RNF26 promoted K11-linked at...
Abstract Introduction Alzheimer’s disease (AD) is the most common cause of dementia, characterized by progressive cognitive decline. Protein biomarkers AD brain pathology, including β-amyloid and Tau, are reflected in cerebrospinal fluid (CSF), yet identification additional linked to other pathophysiologies remains elusive. We recently reported a multiplex tandem-mass tag (TMT) CSF proteomic analysis nearly 3000 proteins, following depletion highly abundant proteins off-line fractionation,...
Gene expression contributes to phenotypic traits and human disease. To date, comparatively less is known about regulators of protein abundance, which also under genetic control likely influences clinical phenotypes. However, identifying quantifying allele-specific abundance by bottom-up proteomics challenging since single nucleotide variants (SNVs) that alter sequence are not considered in standard databases. address this, we developed the GenPro software used it create personalized...
RNA editing is a feature of maturation resulting in the formation transcripts whose sequence differs from genome template. Brain may be altered Alzheimer's disease (AD). Here, we analyzed data 1,865 brain samples covering 9 regions 1,074 unrelated subjects on transcriptome-wide scale to identify inter-regional differences editing. We expand list known events by identifying 58,761 previously unreported events. note that only small proportion these are found at protein level our proteome-wide...
Compared with whole-cell proteomic analysis, subcellular analysis is advantageous not only for the increased coverage of low abundance proteins but also generating organelle-specific data containing information regarding dynamic protein movement. In present study, peroxisome-enriched fractions from Sendai virus (SeV)-infected or uninfected HepG2 cells were obtained and subjected to quantitative proteomics analysis. We identified 311 that significantly changed by SeV infection. Among these...
Core spliceosome and related RNA-binding proteins aggregate in Alzheimer’s disease (AD) brain even early asymptomatic stages (AsymAD) of disease. To assess the specificity protein aggregation AD, we developed a targeted mass spectrometry approach to quantify broad classes with other pathological including tau amyloid beta (Aβ) detergent insoluble fractions from control, AsymAD, AD Parkinson’s (PD) brain. Relative levels specific across different groups correlated accumulation Aβ aggregates....
Abstract Alzheimer’s disease (AD) features a complex web of pathological processes beyond amyloid accumulation and tau-mediated neuronal death. To meaningfully advance AD therapeutics, there is an urgent need for novel biomarkers that comprehensively reflect these mechanisms. Here we applied integrative proteomics approach to identify cerebrospinal fluid (CSF) linked diverse set pathophysiological in the diseased brain. Using multiplex proteomics, identified >3,500 proteins across 40 CSF...
Here, we report a method for the generation of complementary tryptic (CompTryp) isotope-labeled peptide standards relative and absolute quantification proteins by mass spectrometry (MS). These can be digested in parallel with either trypsin (Tryp-C) or trypsin-N (Tryp-N), to generate peptides that significantly overlap primary sequence having C- N-terminal arginine lysine residues, respectively. As proof concept, an CompTryp standard was synthesized Tau, well-established biomarker...
Abstract Background In the era of evidence-based policy-making (EBPM), scientific outputs and public policy should engage with each other in a more interactive coherent way. Notably, this is becoming increasingly critical preparing for health emergencies. Methods To explore coevolution dynamics between science (SAP), study explored changes in, development of, COVID-19 research early period outbreak China, from 30 December 2019 to 26 June 2020. study, VOSviewer was adopted calculate link...
Protein turnover affects protein abundance and phenotypes. Comprehensive investigation of dynamics has the potential to provide substantial information about gene expression. Here we report a large-scale study in Salmonella Typhimurium during infection by quantitative proteomics. Murine macrophage-like RAW 264.7 cells were infected with SILAC labeled Salmonella. Bacterial extracted after 0, 30, 60, 120, 240 min. Mass spectrometry analyses yielded software program named Topograph was used for...
Abstract Our understanding of the biological changes in brain associated with Alzheimer’s disease (AD) pathology and cognitive impairment remains incomplete. To increase our these changes, we analyzed dorsolateral prefrontal cortex control, asymptomatic AD, AD brains from four different centers by label-free quantitative mass spectrometry weighted protein co-expression analysis to obtain a consensus network brain. This consisted 13 modules. Six modules correlated amyloid-β plaque burden, tau...
To identify cerebrospinal fluid (CSF) biomarkers of AD linked to a diverse set pathophysiological processes in the diseased brain.
Abstract Background Our understanding of the biological changes in brain associated with Alzheimer’s disease (AD) pathology and cognitive impairment remains incomplete. Unbiased discovery proteomic analysis AD can help to increase our these changes. Method We analyzed dorsolateral prefrontal cortex, precuneus, temporal cortex control, asymptomatic AD, brains from multiple different centers by label‐free quantitative mass spectrometry weighted protein co‐expression obtain a consensus network...
Abstract The dynamics of research are a prism that reflects interactions between science, the societal environment, and government policies. Against backdrop ongoing global COVID-19 pandemic, this study explored changes in, development of, in period from December 30, 2019 to April 27, 2020. observes salient change content: an earlier focus on “all patients”, “common symptoms”, “nucleic acid test” was gradually replaced by “children”, “pregnant “severe combination “antibody assay”. Some...
There is a need for novel biomarkers of Alzheimer's disease (AD) that broadly serve as accurate indicators the underlying pathophysiological processes occurring throughout disease. Thus, biomarker discovery should focus on identifying not only targets are easily accessible and reliably measured, but also those with clear associations to most critical mechanisms driving neurodegeneration. To achieve this goal, we used an unbiased proteomic approach quantify protein levels in AD cerebrospinal...
Abstract Background The repeated failures of amyloid‐targeting therapies have underscored the need for additional biomarkers Alzheimer’s disease (AD) that reflect its complex multi‐network dysfunction. Using a network‐based proteomic approach, we previously defined wide range protein system alterations in AD brain. In this study, integrated these brain networks with analysis cerebrospinal fluid (CSF), resulting identification CSF biomarker panels reflective diverse pathophysiology within...