A5035289965- Escherichia coli research studies
- Urinary Tract Infections Management
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Viral-associated cancers and disorders
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Cytomegalovirus and herpesvirus research
- Viral gastroenteritis research and epidemiology
- Enterobacteriaceae and Cronobacter Research
- Pediatric Urology and Nephrology Studies
- Bacterial Genetics and Biotechnology
- Clostridium difficile and Clostridium perfringens research
- Vibrio bacteria research studies
- Infections and bacterial resistance
- Immune Cell Function and Interaction
- Nosocomial Infections in ICU
- Gut microbiota and health
- Viral Infections and Vectors
- Lymphoma Diagnosis and Treatment
- Polyomavirus and related diseases
- Insect symbiosis and bacterial influences
- bioluminescence and chemiluminescence research
- RNA and protein synthesis mechanisms
- Spectroscopy Techniques in Biomedical and Chemical Research
Hospital Infantil de México Federico Gómez
2016-2025
Secretaria de Salud
2024
Zagazig University
2023
Osnabrück University
2023
Egis (France)
2021
Instituto Politécnico Nacional
2016
Simmons University
2005
In recent years, an increase of uropathogenic Escherichia coli (UPEC) strains with Multidrug-resistant (MDR) and Extensively Drug-resistant (XDR) profiles that complicate therapy for urinary tract infections (UTIs) has been observed directly impacted costs extended hospital stays. The aims this study were to determine MDR- XDR-UPEC clinical strains, their virulence genes phylogenetic groups ascertain relationship integrons genetic diversity. From a collection 500 UPEC 103 selected MDR...
Stenotrophomonas maltophilia, an emerging opportunistic pathogen, is widely distributed in the environment resistance mechanisms, and virulence factors of this bacterium facilitate its dissemination hospitals. This study aimed to characterize molecular epidemiology S. maltophilia strains associated with outbreak Children's Hospital México Federico Gómez (HIMFG). Twenty-one clinical were recovered from cultures blood urine samples 10 pediatric patients at emergency department, nine...
Cronobacter spp. are opportunistic pathogens linked to lie-threatening infections in neonates and contaminated powdered infant formula that has been epidemiologically associated with these cases. Clinical symptoms of include necrotizing enterocolitis, bacteremia, meningitis. Flagella from C. sakazakii involved biofilm formation its adhesion epithelial cells. We investigated the role flagella ST1 ST4, malonaticus, muytjensii, turicensis dublinensis during activation cytokines (IL-8, TNF-α,...
Introduction Recurrent urinary tract infections (RUTIs) caused by uropathogenic Escherichia coli are costly public health problems impacting patients’ quality of life. Aim In this work, a comparative genomics analysis three clinical RUTI strains isolated from bladder biopsy specimens was performed. Materials and methods One hundred seventy-two whole genomes E. were selected the NCBI database. The search for virulence factors, fitness genes, regions interest, genetic elements associated with...
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Enterotoxigenic Escherichia coli (ETEC) produces two types of enterotoxins, LTs and STs, as well several colonization factors (CFs), including CS21, CS3 fimbriae, flagellar structures. This study investigated how these structures contribute to ETEC the immune response in HT-29 HuTu-80 intestinal cells. strains with single, double, triple mutations lngA, cstH, fliC genes were generated confirmed using PCR Western blotting. The cells by E9034A strain, which was fully sequenced a hybrid...
Urinary tract infections (UTIs) are a leading cause of illness in children and adults all ages, with uropathogenic Escherichia coli (UPEC) being the primary agent responsible. During colonization subsequent infection urinary (UT), UPEC requires expression genes associated virulence, such as those that encode fimbrial adhesins FimH, PapG, CsgA, well presence TosA protein flagellar appendages bacteria. However, for to be successful, must overcome host’s immunological barriers, physical...
ABSTRACT Enterotoxigenic Escherichia coli (ETEC) is the leading bacterial cause of diarrhea in developing world, as well most common traveler's diarrhea. The main hallmarks this type bacteria are expression one or more enterotoxins and fimbriae used for attachment to host intestinal cells. Longus a pilus produced by ETEC. These grown pleuropneumonia-like organism (PPLO) broth at 37°C 5% CO 2 longus, showing that assembly pili depend on growth conditions composition medium. To explore role...
Background Enterohemorrhagic Escherichia coli (EHEC) O157:H7, the causative agent of hemorrhagic colitis and hemolytic uremic syndrome (HUS), produces long bundles type IV pili (TFP) called (HCP). HCP are capable mediating several phenomena associated with pathogenicity: i) adherence to human bovine epithelial cells; ii) invasion iii) hemagglutination rabbit erythrocytes; iv) biofilm formation; v) twitching motility; vi) specific binding laminin fibronectin. composed a 19 kDa pilin subunit...
Curli, a type of fimbriae widely distributed in uropathogenic Escherichia coli (UPEC), are involved adhesion to human bladder cell surfaces and biofilm development. The role UPEC curli was evaluated murine model urinary tract infection. aim this study establish the C57BL/6 mice transurethrally infected with curli-producing non-curli-producing strains. We confirmed that enhanced colonization tract, resulting damage both kidney. Intranasal immunization recombinant CsgA protein protected...
The Acinetobacter calcoaceticus-baumannii (Acb) complex is regarded as a group of phenotypically indistinguishable opportunistic pathogens responsible for mainly causing hospital-acquired pneumonia and bacteremia. aim this study was to determine the frequency isolation species that constitute Acb complex, well their susceptibility antibiotics, distribution at Hospital Infantil de Mexico Federico Gomez (HIMFG). A total 88 strains previously identified by Vitek 2®, 40 baumannii 48 were...
Methicillin-resistant Staphylococcus aureus (MRSA) is an important opportunistic pathogen that causes both healthcare- and community-acquired infections. An increase in the incidence of these infections may lead to a substantial change rate vancomycin usage. Incidence reduced susceptibility has been increasing worldwide for last few years, conferring different levels resistance as well producing changes cell wall structure. The aim present study was determine effect on thickening clinical...
The Hospital Infantil de México Federico Gómez (HIMFG) is a tertiary care hospital in Mexico City where Escherichia coli frequently isolated from the urine samples of pediatric patients with urinary tract infections. A collection 178 (UEc) isolates associated complicated and uncomplicated infections were evaluated this study. patterns resistance to 9 antibiotic classes showed that 60.7% UEc had highly multidrug-resistant (MDR) profile. Genetic diversity analyses high variability revealed 16...
Enterococcus faecium has recently emerged as a multidrug-resistant nosocomial pathogen involved in outbreaks worldwide. A high rate of resistance to different antibiotics been associated with virulent clonal complex 17 isolates carrying the esp and hyl genes purK1 allele. Twelve clinical vancomycin-resistant (VREF) were obtained from pediatric patients at Hospital Infantil de México Federico Gómez (HIMFG). Among these VREF isolates, 58.3% (7/12) recovered urine, while 41.7% (5/12)...
Hand hygiene is the most important strategy for preventing healthcare-associated infections (HCAIs); however, impact of hand in middle-income countries has been poorly described. In this work, we describe programme 'Let's Go 100' on adherence, HCAIs rates and multidrug-resistant (MDR) bacteria, including molecular typing methicillin-resistant Staphylococcus aureus (MRSA) strains.A multimodal, hospital-wide was implemented from 2013. involved all healthcare workers encompassed education,...
Urinary tract infections (UTIs) are associated with high rates of morbidity and mortality worldwide, uropathogenic Escherichia coli (UPEC) is the main etiologic agent. Fimbriae assembled on bacterial surface essential for adhesion in urinary epithelium. In this study, FimH, CsgA, PapG adhesins were fused to generate biomolecules as potential target vaccines against UTIs. The fusion protein design was generated using bioinformatics tools, template gene sequences synthesized by GenScript...
Acinetobacter baumannii is an opportunistic pathogen and one of the primary etiological agents healthcare-associated infections (HAIs). A. are difficult to treat due intrinsic acquired antibiotic resistance strains this bacterium, which frequently limits therapeutic options. In study, five (810CP, 433H, 434H, 483H, A-2), all were isolated from a child with leukemia M2, characterized through susceptibility profiling, detection genes encoding carbapenem hydrolyzing oxacillinases, pulsed-field...
Urinary tract infections (UTIs) are a public health problem in Mexico, and uropathogenic
Uropathogenic Escherichia coli (UPEC) has increased the incidence of urinary tract infection (UTI). It is cause more than 80% community-acquired cystitis cases and 70% uncomplicated acute pyelonephritis cases. The present study describes molecular epidemiology UPEC O25b clinical strains based on their resistance profiles, virulence genes, genetic diversity. Resistance profiles were identified using Kirby-Bauer method, including phenotypic production extended-spectrum β-lactamases (ESBLs)...
Staphylococcus aureus is an opportunistic pathogen that colonizes human hosts and causes a wide variety of diseases. Two interacting regulatory systems called agr (accessory gene regulator) sar (staphylococcal accessory are involved in the regulation virulence factors. The aim this study was to evaluate effect vancomycin on hld spa expression during exponential post-exponential growth phases multidrug-resistant (MDR) S. aureus.Antibiotic susceptibility evaluated by standard microdilution...
Background: Enterotoxigenic Escherichia coli (ETEC) colonize the human intestinal mucosa using pili and non-pili colonization factors (CFs). CS21 (also designated Longus) is one of most prevalent CFs encoded by a 14 kb lng DNA cluster located in virulence plasmid ETEC; yet limited information available on prevalence positive ETEC isolates different countries. The aim this study was to evaluate among clinical from Mexican Bangladeshi children under five years old with diarrhea determine...