Abstract Background The efficacy of Centella asiatica for incision and burn wounds are not fully understood. Here, we report the wound healing activities sequential hexane, ethyl acetate, methanol, water extracts in partial-thickness models rats. Methods Male Sprague–Dawley rats weighing 250–300 g were randomly divided into groups. Each group was stratified seven subgroups: (1) untreated; (2) NSS-; (3) Tween 20 ® - (vehicle control); (4) hexane extract-; (5) acetate (6) methanol (7) aqueous...

In order to gain insight into neuroprotective effects of ECa 233, a standardized extract Centella asiatica, previously demonstrated in animal models memory impairment induced by transient global ischemia or intracerebroventricular injection β-amyloid, the effect 233 on neurite outgrowth human IMR-32 neuroblastoma cell line was investigated.Cells were seeded and incubated with various concentrations 233. Morphometric analysis carried out measurement longest growth cells at 24 48 h....

ECa 233, a standardized extract of Centella asiatica, has been found to exhibit various positive neurological effects and have good safety profile. The present study aimed explore the disposition kinetics containing madecassoside (53.1 %) asiaticoside (32.3 %), in rats. was intravenously or orally administered at doses from 50 200 mg/kg. Plasma, tissues, urine, feces were collected time points 0 48 h after dosing. levels asiaticoside, as well their postulated triterpenic metabolites,...

Centella asiatica is widely considered the most important medicinal plant for treating and relieving skin diseases. Recently developed standardized extract of ECa 233 has demonstrated positive effects on wound healing incision burn in rats. However, knowledge associated with mechanism was scare. Therefore, this study aimed to investigate effect underlying molecular mechanisms migration a human keratinocyte cell line (HaCaT) using scratch assay. Formation filopodia, key protein as well...

The aim of this study was to investigate the safety and pharmacokinetic profiles a newly developed, standardized extract Centella asiatica (ECa 233) capsule in healthy Thai volunteers. This designed as an open-labeled, 2-sequence dosage, single- repeated-dose investigated under fasting conditions. Plasma concentrations parent compounds their relative acid metabolites were measured parameters calculated using noncompartmental analysis. Tolerability assessed based on physical examinations,...

To determine the efficacy of Centella asiatica extract, ECa233, on alleviating pain symptoms and functional improvement acute temporomandibular disorders (TMD). A randomized, double-blind, placebo-controlled, pilot clinical trial was performed using 23 adults with TMD. They were randomly assigned into four treatment groups, an ibuprofen (positive control) group, two test groups ECa233 each 250 mg, 500 mg extracts, a placebo (negative group. All subjects requested to self-administer...

1. ECa 233, the standardised extract of Centella asiatica, contains not less than 80% triterpenoid glycosides, in a madecassoside:asiaticoside ratio 1.5 (±0.5):1. 2. The pharmacokinetic comparison madecassoside and asiaticoside was performed rats following intravenous oral administration or an equivalent dose individual compounds. Blood, tissues, urine faeces were collected after dosing to determine drug metabolite levels using liquid chromatography-tandem mass spectrometry. 3. Our study...

Acute toxicity study of C entella asiatica standardized extract ECa 233 was conducted by an oral administration 10.0 g/kg into 10 male and female mice.The at the given dose did not cause any toxic signs death within observation period 14 days.Sub-chronic has been investigated in four groups Wistar rats, each 24 rats (12 sex).Control group orally distilled water three experimental were administered with doses 10, 100, 1,000 mg/kg/day for 90 days.All 233-treated showed no difference regards to...

Background:Centella asiatica, a medicinal plant, has been used traditionally to promote wound healing. Its efficacy on promoting postlaser resurfacing healing is lacking. Methods: Thirty individuals with facial acne scars underwent treatment 2940 nm Er:YAG laser. Half side of the face was randomized receive 0.05% w/w ECa 233 gel, standardized extract C. and other half placebo gel. The gels were applied four times daily for 7 days then twice 3 months. Erythema, melanin, texture index (TI)...

The herb Centella asiatica has long been considered a memory tonic. A recent review found no strong evidence for improvement of cognitive function, suggesting negative results were due to limitations in dose, standardization and product variation. We used standardized extract C. (ECa 233) study behavioral, cellular molecular effects on learning enhancement. ECa 233 (10, 30, 100 mg/kg) was given orally normal rats twice day 30 days. the Morris water maze test spatial performed acute brain...

Background and purpose: The anticancer drugs used for oral cancer treatment present many disadvantages, such as low solubility, permeability, poor bioavailability. However, the activity of ECa 233 has not been widely studied. Therefore, was investigated in this study. Experimental approach: MTT assay carried out to determine cell viability. Characterizations apoptosis were monitored using DAPI FDA staining Hoechst 33258 AO staining. Confirmation apoptosis-induced KON cells done annexin...

A current anti-inflammatory agent often targets the prevention of inflammatory disorder development. The standardized Centella asiatica ECa 233 extract has been previously reported for effect. This study aimed to investigate its effect and mechanisms in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, nitric oxide (NO) reactive oxygen species (ROS) production enzyme-linked immunosorbent assay (ELISA)...

To investigate the effect of a standardized extract Centella asiatica (ECa 233), which has anti-inflammatory properties, on local expression transient receptor potential vanilloid 1 (TRPV1), acid-sensing ion channel subunit 3 (ASIC3), and calcitonin gene-related peptide (CGRP) in temporomandibular joint (TMJ) structure 21 days after injecting TMJ with complete Freund's adjuvant (CFA).A mouse model was induced by analyzing CFA-injected 7, 14, 21. We assessed histology osteoarthritis cartilage...

The effects of the heat-stable enterotoxin Escherichia coli (STa) on intestinal alkaline secretion and transepithelial electric potential difference (PD) were investigated in vivo denervated segments rat jejunum, ileum, proximal colon. STa caused a significant increase jejunum but not ileum or jejunal effect may be ascribed to stimulation bicarbonate and/or an inhibition Na+/H+ exchange. With regard PD, marked rise colonic whereas only small response was found jejunum. No PD seen ileum....

Centella asiatica has been used as a culinary vegetable or medicinal herb. In this study, the hepatoprotective effect of standardized extract C. (ECa233) in rotenone-treated rats was examined using GC-MS-based metabolomic approach. ECa233 contains >80% triterpenoids with ratio madecassoside to asiaticoside 1.5(±0.5):1. Rats were randomly divided into three groups (with six rats/group): sham negative control, rotenone positive control and test group. group received 10 mg/kg orally for 20...

Abstract ECa 233 is a standardized extract of Centella asiatica, characterized as white powder containing triterpenoid glycosides not less than 80% with ratio madecassoside to asiaticoside 1.5±0.5:1. Although pharmacological and toxicological profiles have been successively reported, the pharmacokinetic data needed for further therapeutic development are fully elucidated. This study aimed investigate pharmacokinetics multiple oral dosing at 100 mg/kg/day 7 days in rats. Plasma, tissues,...

GABAergic intercalated neurons of amygdala (ITCs) have recently been shown to be important in the suppression fear‐like behavior. Effects ECa233 (a standardized extract Centella asiatica ), previously demonstrated anxiolytic activity, were then investigated on ITCs. Cluster expressing fluorescence GFP was identified GAD67‐GFP knock‐in mice. We found that medial paracapsular ITC exhibiting certain intrinsic electrophysiological properties similar those by at same location C57BL/6J Therefore,...

During oral surgery and temporomandibular joint repositioning, pain hypersensitivity often occurs due to irritation or inflammation of the nerve endings in orofacial region. This study aimed investigate effects ECa 233, a Centella asiatica-standardized extract, on development mechanical hyperalgesia allodynia induced by chronic constriction injury infraorbital mice.

A pH-stat technique was used to investigate the effects of cholera toxin (CT) on alkaline secretion from denervated intestines (jejunum, ileum, colon) in anaesthetized rats. Transepithelial potential difference (PD) also followed some experiments. CT, given intraluminally, caused a marked increase jejunal secretion, whereas only small effect observed ileum and no apparent noted proximal colon. The pronounced found be inhibited by 10-25% hexamethonium (10 mg kg-1 body wt i.v.) similarly...