A5101404025- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Proteins in Food Systems
- Diversity and Career in Medicine
- Electrohydrodynamics and Fluid Dynamics
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- Pesticide Exposure and Toxicity
- Geological and Geochemical Analysis
- Membrane Separation Technologies
- Microbial Metabolites in Food Biotechnology
- Membrane-based Ion Separation Techniques
- Metabolomics and Mass Spectrometry Studies
- Microencapsulation and Drying Processes
- Geochemistry and Geologic Mapping
- Hospital Admissions and Outcomes
- Bacteriophages and microbial interactions
- Healthcare professionals’ stress and burnout
- Hydrocarbon exploration and reservoir analysis
- Chromium effects and bioremediation
- Biotin and Related Studies
- Polyamine Metabolism and Applications
- Enzyme-mediated dye degradation
- Dental Education, Practice, Research
University of Hohenheim
2016-2020
Institut für Pharmakologie und Toxikologie der Bundeswehr
2014-2018
Ludwig-Maximilians-Universität München
2015-2018
Center for Integrated Protein Science Munich
2015-2018
Indiana University School of Medicine
2008-2017
Indiana University – Purdue University Indianapolis
2008-2017
Institute of Pharmacology
2014
Friedrich Schiller University Jena
2001-2009
Translation arrest by polybasic sequences induces ribosome stalling, and the product is degraded ribosome-mediated quality control (RQC) system. Here we report that ubiquitination of 40S ribosomal protein uS10 E3 ubiquitin ligase Hel2 (or RQT1) required for RQC. We identify a RQC-trigger (RQT) subcomplex composed RNA helicase-family Slh1/Rqt2, ubiquitin-binding Cue3/Rqt3, yKR023W/Rqt4 The defects in RQC RQT mutants correlate with sensitivity to anisomycin, which stalls at rotated form....
During protein synthesis, ribosomes become stalled on polyproline-containing sequences, unless they are rescued in archaea and eukaryotes by the initiation factor 5A (a/eIF-5A) bacteria homologous EF-P. While a structure of EF-P bound to 70S ribosome exists, structural insight into eIF-5A 80S has been lacking. Here we present cryo-electron microscopy reconstruction yeast at 3.9 Å resolution. The reveals that unique functionally essential post-translational hypusine modification reaches...
Getting rid of faulty mRNA The cell monitors the health its mRNAs, destroying those that are or damaged. Destruction by exosome complex prevents them from being used to synthesize deranged and potentially dangerous proteins. Schmidt et al. determined structure Ski helicase complex, which guides RNAs destruction machinery in association with a mRNAbound ribosome. end is threaded ribosome into heart helicase, whence message would be channeled maw complex. Science , this issue p. 1431
Mechanistic understanding of eukaryotic ribosome formation requires a detailed structural knowledge the numerous assembly intermediates, generated along complex pathway. Here, we present structure late pre-40S particle at 3.6 Å resolution, revealing in molecular detail how factors regulate timely folding pre-18S rRNA. The shows that, rather than sterically blocking 40S translational active sites, associated Tsr1, Enp1, Rio2 and Pno1 collectively preclude their final maturation, thereby...
Human butyrylcholinesterase (hBChE) is a serine hydrolase (EC 3.1.1.8) present in all mammalian tissues and the bloodstream. Similar to acetylcholinesterase, enzyme reacts with organophosphorus compounds (OP) like nerve agents or pesticides that cause inhibition (BChE adducts). These adducts represent valuable biomarkers for analytical verification of OP exposure. For establishment these mass spectrometry based methods sufficient amounts hBChE high purity are required. Unfortunately,...
Atropine (Atr) and scopolamine (Scp) are toxic secondary plant metabolites of species within the Solanaceae genus that can accidentally or intentionally reach food store chain by inaccurate harvesting any material, e.g. for herbal tea infusions. Ingestion may cause severe anticholinergic poisoning thus requiring risk-oriented determination in beverages. The suitability matrix-assisted laser desorption/ionization time-of-flight (tandem) mass spectrometry, MALDI-TOF MS(/MS), should be...
This study aimed at determining sol–gel transition temperatures of microfiltered skim milk retentates for different protein levels (6, 8 and 10% (w/w)) a wide pH range (native to 4.6) by means small‐amplitude oscillatory shear rheology. For 5.4 5.0, the decreased significantly with increasing content, which did not differ 4.8 4.6. The (w/w) 4.6 were 58.4 °C 10.9 °C, respectively.
Volatile components in magma strongly influence many physical properties of melts and minerals. The temperature resolved degassing analysis volcanic crystalline vitreous rocks gives detailed information about volatile compounds the melt. Aspecial high-temperature mass-spectrometry device combination with a thermo-balance allows quantitative determination different species. It enables differentiation between primary gas content released from decomposition secondary alteration products....