A5007088144- Growth Hormone and Insulin-like Growth Factors
- Cytokine Signaling Pathways and Interactions
- Digestive system and related health
- Metabolism, Diabetes, and Cancer
- Cancer, Hypoxia, and Metabolism
- Pancreatic function and diabetes
- Lipid metabolism and disorders
- PI3K/AKT/mTOR signaling in cancer
- Liver physiology and pathology
- Galectins and Cancer Biology
- Colorectal Cancer Surgical Treatments
- Endoplasmic Reticulum Stress and Disease
- Urticaria and Related Conditions
- Cardiac, Anesthesia and Surgical Outcomes
- Animal Genetics and Reproduction
- Reproductive System and Pregnancy
- Protein Tyrosine Phosphatases
- Colorectal Cancer Screening and Detection
- Thyroid Disorders and Treatments
- Retinoids in leukemia and cellular processes
- Pituitary Gland Disorders and Treatments
- thermodynamics and calorimetric analyses
- Diabetes and associated disorders
- FOXO transcription factor regulation
- Pancreatic and Hepatic Oncology Research
Instituto de Química y Fisicoquímica Biológicas
2015-2024
University of Buenos Aires
2015-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2014-2024
Universidad de Lima
2022
Osasun Sistemen Ikerketa Institutua
2021
Hospital Universitario Fundación Alcorcón
2021
Hospital Clínico San Carlos
2021
Hospital Universitario La Paz
2021
Hospital Universitario Araba
2021
Corporació Sanitària Parc Taulí
2021
Growth hormone (GH) overexpression throughout life in transgenic mice is associated with the development of liver tumors at old ages. The preneoplastic pathology observed young adult GH-overexpressing similar to that present humans high risk hepatic cancer. To elucidate molecular pathogenesis underlying pro-oncogenic induced by prolonged exposure elevated GH levels, activation and expression several components signal transduction pathways have been implicated hepatocellular carcinogenesis...
Lecithins, mainly composed of the phospholipids phosphatidylcholines (PC), have many different uses in pharmaceutical and clinical field. PC are involved structural biological functions as membrane trafficking processes cellular signaling. Considering increasing applications lecithin-based nanosystems for delivery therapeutic agents, aim present work was to determine effects phosphatidylcholine nanoparticles over breast cancer proliferation dispersions at 0.01 0.1% (w/v) prepared buffer pH...
Chronically elevated levels of GH in GH-transgenic mice result accelerated growth and increased adult body weight. We have previously described that the GH-induced JAK2/STAT5-signaling pathway is desensitized liver transgenic overexpressing GH. However, these animals present circulating IGF-I levels, hepatic GHR expression, organomegaly due to hypertrophy hyperplasia, which frequently progress hepatomas as age, indicating action on not prevented. In study, we evaluated other GH-signaling...
Epidermal growth factor (EGF) is a key regulator of cell survival and proliferation involved in the pathogenesis progression different types cancer. The EGF receptor (EGFR) activated by binding specific ligand but also transactivation triggered factors including GH. Chronically, elevated GH levels have been associated with hepatocellular carcinoma. Considering involvement their signaling crosstalk, objective present study was to analyze modulatory effects on liver. For this purpose,...
The reliability of reverse transcription-quantitative PCR (RT-qPCR) results in gene expression studies depends on the approaches used to account for non-biological variations. In order find a proper normalization strategy study genes related growth hormone signaling skeletal muscle growing mice, nine unrelated were evaluated as internal controls. According most algorithms–geNorm, Comparative ΔCq method, NormFinder and BestKeeper–GSK3B, YWHAZ, RPL13A RN18S found stable. However, relative...
Acromegaly is associated with cardiac hypertrophy, which believed to be a direct consequence of chronically elevated GH and IGF1. Given that insulin important for growth function, considering excess induces hyperinsulinemia, resistance, alterations, it interest study sensitivity in this tissue under chronic conditions GH. Transgenic mice overexpressing present cardiomegaly perivascular interstitial fibrosis the heart. Mice received an injection, heart was removed after 2 min, immunoblotting...
High continuous GH levels in vivo produce desensitization of the Janus tyrosine kinase 2 (JAK2)/signal transducer and activator transcription 5 (STAT5) pathway signaling liver. To evaluate mechanisms involved this desensitization, transgenic mice overexpressing bovine were used. In these animals, receptor membrane-associated JAK2 are increased 4.5- 6-fold, respectively. However, JAK2. STAT5a -5b do not become phosphorylated response to stimulus, nor STAT proteins recruited membranes,...
GH/STAT5 signaling is desensitized in the liver adult transgenic mice overexpressing GH; however, these animals present greater body size. To assess whether STAT5 pathway active during growth period animals, and how modulators participate this process, growing normal siblings were evaluated. does not respond to an acute GH-stimulus, but displays higher basal phosphorylation livers of GH-overexpressing mice. GH receptor positive glucocorticoid HNF1 display abundance supporting activity STAT5....
We have examined the regulation of hepatic growth hormone receptors (GH-R) and serum GH binding proteins (GHBP) in transgenic mice expressing an antagonist bovine (bGH), G119K-bGH, consequently exhibiting a suppressed dwarf phenotype. Specific GHBP could be measured mouse only by immunological methods (RIA), because these very high concentration mutated bGH circulation (> 1 microgram/ml) and, therefore, almost all is bound to G119K-bGH cannot quantitated assays. The concentrations were...
Current GH administration protocols imply frequent s.c. injections, resulting in suboptimal compliance. Therefore, there is interest developing delivery systems for sustained release of the hormone. However, has different actions depending on its continuous or pulsatile plasma concentration pattern. levels and circulating patterns could be involved regulation epidermal growth factor receptor (EGFR) expression liver. Aberrant this and/or hyperactivation been associated with pathogenesis types...
Growth hormone (GH) is a pleiotropic that triggers STATs, ERK1/2 and Akt signaling, related to cell growth proliferation. Transgenic mice overexpressing GH present increased body size, with disproportionate liver enlargement due hypertrophy hyperplasia of the hepatocytes. We had described enhanced mitogenic signaling in young adult transgenic mice. now evaluate activation these cascades during period relate them morphological alterations found. Signaling mediators, cycle regulators...
The effects of continuous high GH levels on signal transduction through the receptor (GHR)/Janus kinase 2 (JAK2)/signal transducer and activator transcription 5 (STAT5) pathway as well desensitization this by suppressors cytokine signaling (SOCS) were studied in transgenic mice overexpressing GHRH. In mice, hepatic GHR 4.5-fold higher than normal animals, whereas protein contents JAK2, STAT5a, STAT5b did not vary. This same pattern was found for basal tyrosine phosphorylation (PY-): PY-GHR...
Abstract Background The aim of this study was to assess factors associated with outcomes after surgery for colorectal cancer and design internally validate a simple score predicting perioperative mortality. Methods Patients undergoing primary invasive in 22 centres Spain between June 2010 December 2012 were included. Clinical variables up 30 days collected prospectively. Multiple logistic regression techniques applied risk developed. Hosmer–Lemeshow test the area under receiver operating...
Transgenic mice overexpressing growth hormone (GH) spontaneously develop liver tumors, including hepatocellular carcinoma (HCC), within a year. The preneoplastic pathology in these recapitulates that observed humans at high risk of developing hepatic cancer. Although increased expression galectin 1 (GAL1) tissue is associated with HCC aggressiveness, link between this glycan-binding protein and hormone-related tumor development has not yet been explored. In study, we investigated GAL1 during...
The decay curve of labeled growth hormone (GH) in the plasma followed a three-compartment model and could be described by equation: concentration = Ae-alpha t + Be-beta Ce-gamma t, where A, B, C are y-intercepts alpha, beta, gamma compartments. When 125I-labeled ovine prolactin (oPRL) was injected, t. Formation bovine-GH-binding protein (GHBP) complexes with somatogenic characteristics demonstrated serum both normal GH transgenic mice. In contrast, 125I-oPRL unable to form this type any mice...