In recent years, rheological measurements of cells and tissues at physiological pathological stages have become an essential method to determine how forces changes in mechanical properties contribute disease development progression, but there is no standardization this procedure so far. study, we evaluate the potential nanoscale atomic force microscopy (AFM) macroscopic shear rheometry assess healthy cancerous human colon tissues. The direct comparison tissue behavior under uniaxial...

Background Microbial biofilms, as a hallmark of cystic fibrosis (CF) lung disease and other chronic infections, remain desirable target for antimicrobial therapy. These biopolymer-based viscoelastic structures protect pathogenic organisms from immune responses antibiotics. Consequently, treatments directed at disrupting biofilms represent promising strategy combating biofilm-associated infections. In CF patients, the viscoelasticity is determined mainly by their polymicrobial nature...

This study explores the rheological properties of polydimethylsiloxane (PDMS) composites with glass beads (GBs) to replicate compression-stiffening behavior biological tissues. The mechanical soft tissues arise from interactions between extracellular matrix (ECM) and embedded cells. To mimic this, PDMS was used as a polymeric matrix, while rigid GBs acted non-deformable inclusions facilitating stress redistribution. 10%, 20%, 30% GB concentrations were fabricated. Rheological analysis...

Polycaprolactone (PCL) and polylactide (PLA) are the two most common biodegradable polymers with potential use in oral applications. Both undergo mainly slow hydrolytic degradation human body. However, specific conditions of cavity, like elevated temperature, low pH, presence saliva affect rate hydrolysis. The study examined properties solid samples PCL PLA subjected to phosphate buffered saline (PBS) artificial (AS) at temperatures 37 or 42 °C, pH values 2 7.4. A number tests were...

Polylactide (PLA) and polycaprolactone (PCL) are biodegradable bioabsorbable thermoplastic polymers considered as promising materials for oral applications. However, any abiotic surface used, especially in areas naturally colonized by microorganisms, provides a favorable interface microbial growth biofilm development. In this study, we investigated the formation of C. krusei S. mutans on PLA PCL immersed artificial saliva. Using microscopic (AFM, CLSM) observations spectrometric...

Saliva plays a crucial role in oral cavity. In addition to its buffering and moisturizing properties, saliva fulfills many biofunctional requirements, including antibacterial activity that is essential assure proper microbiota growth. Due numerous extra- intra-systemic factors, there are disorders of secretion, leading dryness. substitutes used such situations must meet demands. This study was design evaluate the effect core-shell magnetic nanoparticles (MNPs) adding (gold-coated...

Extracellular polymeric substances (EPS) produced by bacteria, as they form a biofilm, determine the stability and viscoelastic properties of biofilms prevent antibiotics from penetrating this multicellular structure. To date, studies demonstrated that an appropriate optimization chemistry morphology nanotherapeutics might provide favorable approach to control their interaction with EPS and/or diffusion within biofilm matrix. Targeting biofilms' EPS, which in certain conditions can adopt...

Voice prosthesis implantation with the creation of a tracheoesophageal fistula is gold standard procedure for voice rehabilitation in patients after total laryngectomy. All implanted (VP) have biofilms fungi and bacteria grow on their surface. Biofilm colonization one main reasons VP degradation that can lead to dysfunction, which increases high risk pneumonia. In 20-month evaluation period, 129 cases prostheses replacement procedures were investigated. Microbiological examination revealed...

Selected mechanical and biological properties of biodegradable elastomeric poly(ester-carbonate-urea-urethane)s (PECUUs) point towards their potential to be applied as scaffolds in tissue engineering. Here we explore medical applicability taking into account hemocompatibility cytotoxicity. The influence the ester monomer (derivatives adipic succinic acids), well diisocyanate type (IPDI HDI) on investigated PECUUs is presented. presence aliphatic diisocyanates, cyclic IPDI or linear HDI,...

The paper presents the results of research fretting process 316 austenitic stainless steel in environment natural saliva and mucin-based preparations. aim work was evaluation synthetic preparations on biomaterial wear during fretting-corrosion. process, oscillatory micro-movements conditions, occurs joints removable dentures, especially chewing phase. Fretting usually leads to intensification fatigue damage processes materials. Experimental research, through rheological, fretting,...

Deoxyribonucleic acid (DNA) evolved as a tool for storing and transmitting genetic information within cells, but outside the cell, DNA can also serve "construction material" present in microbial biofilms or various body fluids, such cystic fibrosis, sputum, pus. In work, we investigate mechanics of formed from Pseudomonas aeruginosa Xen 5, Staphylococcus aureus 30, Candida albicans 1408 using oscillatory shear rheometry at different levels compression recreate these systems entangled cells....

: Histopathological examination enables visualization of morphological changes in cells and tissues. In recent years, there has been increasing interest assessing the mechanical properties tissues that cannot be determined by standard histopathological examinations. Mechanobiology is crucial human physiology holds promise for uncovering new diagnostic markers disease processes such as carcinogenesis inflammation. this study, we concentrated on measuring appendix biopsy specimens to identify...

Abstract Blood is a vital part of our circulatory system. It responsible for transporting oxygen and nutrients, regulating body temperature, fighting infections. However, any imbalances in blood composition or disruptions the production process can affect body’s overall functioning. Anemia one most common diseases diagnosed worldwide. characterized by deficiency red cells hemoglobin, which reduces ability to transport oxygen. To address this issue, researchers are developing substitutes with...

Magnetic micro- and nanoparticles (MPs)-based composite materials are widely used in various applications electronics, biotechnology, medicine. This group of silicone composites have advantageous magnetic mechanical properties as well sufficient flexibility biocompatibility. These can be applied medicine for biological sensing, drug delivery, tissue engineering, remote-controlled microrobots operating vivo. In this work, the polydimethylsiloxane (PDMS)-based with different percentages (30...

Xerostomia is a disease characterized by chronic feeling of dry mouth associated with reduced amount and quality saliva. When caused salivary glands irreversible damage as result anti-cancer therapies, it requires the administration artificial saliva that will restore physiological functions natural In addition to moisturizing lubricating effects, also important antimicrobial properties healthy Patients suffering from xerostomia occurring cancer treatment, are often immunocompromised...

Natural saliva performs many important functions in the human body. Maintaining its production at a constant level is very aspect of proper functioning oral cavity. However, due to certain factors, reduction salivary secretion observed. In such situations, substitutes are used, which should have similar properties natural saliva. Such fulfilled by our preparations based on porcine gastric mucin. Its oligosaccharide chains affect physicochemical and rheological substitutes. work, influence...

Saliva plays a crucial role in maintaining homeostasis not only within the oral cavity but also further sections of gastrointestinal tract. Pleiotropic properties saliva include participation digestion carbohydrates, cleansing and moisturizing cavity, composition microbiome. The result impaired function salivary gland is reduced salivation – hyposalivation, leading to dry mouth xerostomia. It established that numerous physiological factors (age, sex, weight change) pathological (polytherapy,...