A5018217496- Renal Diseases and Glomerulopathies
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- HIV Research and Treatment
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Blood groups and transfusion
- Platelet Disorders and Treatments
- Celiac Disease Research and Management
- Viral gastroenteritis research and epidemiology
- Transgenic Plants and Applications
- Pediatric health and respiratory diseases
- Influenza Virus Research Studies
- Diabetes and associated disorders
- Galectins and Cancer Biology
- Infant Nutrition and Health
- Autoimmune Bullous Skin Diseases
- Complement system in diseases
- Viral Infections and Immunology Research
- Respiratory viral infections research
- HIV/AIDS Research and Interventions
- Eosinophilic Esophagitis
University of Alabama at Birmingham
2015-2024
Aarhus University
2022
University of Alabama
1995-2012
Czech Academy of Sciences, Institute of Microbiology
2008-2011
University of Helsinki
2010
University of Turku
2010
Helsinki University Hospital
2010
University of Iowa
1998
Nihon University
1990-1996
University of Alabama at Birmingham Hospital
1991
IgA nephropathy (IgAN) is characterized by circulating immune complexes composed of galactose-deficient IgA1 and a glycan-specific IgG antibody. These deposit in the glomerular mesangium induce mesangioproliferative glomerulonephritis characteristic IgAN. To define precise specificities molecular properties antibodies, we generated EBV-immortalized IgG-secreting lymphocytes from patients with IgAN found that secreted formed glycan-dependent manner. We cloned sequenced heavy- light-chain...
Although high serum levels of galactose-deficient IgA1 (an important biomarker IgA nephropathy (IgAN)) are found in most patients with IgAN, their relationship to disease severity and progression remains unclear. To help clarify this we prospectively enrolled 275 IgAN followed them for a median 47 months (range 12–96 months). Serum galactose–deficient was measured at the time diagnosis using lectin-based ELISA, renal survival modeled Cox proportional hazards method. The were higher compared...
Abstract The purpose of this study was to investigate whether oral tolerance, defined as Ag-specific immunologic unresponsiveness after Ag feeding, could be induced in humans prolonged ingestion. Eight adult volunteers ingested a total dose 0.5 g keyhole limpet hemocyanin (KLH) followed by subcutaneous immunization with KLH. controls received only the immunization. In group fed KLH, there significant reduction KLH-specific T cell proliferation (p = 0.04) and delayed skin test responses 0.07)...
The finding that ingestion of antigens results in the selection induction IgA antibodies external secretions suggests antigen sensitizes Peyer's patch lymphoid cells, which migrate to mucosal sites and generate local secretory (S-IgA) antibody responses. Evidence for a common immune system humans has been scanty because difficulty demonstrating migratory behavior cells. In present study, peripheral blood mononuclear cells (PBMC) from human volunteers who had ingested capsules containing...
Aberrant glycosylation of IgA1 plays an essential role in the pathogenesis IgA nephropathy. This abnormality is manifested by a deficiency galactose hinge-region O-linked glycans IgA1. Biosynthesis these occurs stepwise fashion beginning with addition N-acetylgalactosamine enzyme N-acetylgalactosaminyltransferase 2 and continuing either β1,3-galactosyltransferase or terminal sialic acid N-acetylgalactosamine–specific α2,6-sialyltransferase. To identify molecular basis for aberrant...
IgA nephropathy (IgAN) is a complex trait determined by genetic and environmental factors. Most IgAN patients exhibit characteristic undergalactosylation of the O-glycans IgA1 hinge region, which promotes formation glomerular deposition immune complexes. It not known whether this aberrant glycosylation result an acquired or inherited defect, presence glycoforms alone can produce IgAN. A newly validated lectin enzyme-linked immunosorbent assay (ELISA) was used to determine serum level...
2882MetricsTotal Downloads288Last 6 Months58Last 12 Months99Total Citations2Last Months0Last Months0View all metrics
Previous attempts to induce mucosal antibodies in rhesus monkeys by enteric immunization have resulted only modest and short-lived responses, dominated immunoglobulin M (IgM) the plasma. In this study, two groups of were immunized intranasally three times at 2-week intervals with a bacterial protein antigen (AgI/II) either chemically coupled or mixed B subunit cholera toxin (CT), known potent immunogen carrier for other immunogens. Cells secreting antibodies, predominantly IgA isotype,...
Healthy adult volunteers were immunized by parenteral or oral routes with trivalent inactivated influenza vaccine (A/Chile/1/83 (H1N1), A/Mississippi/1/85 (H3N2), and B/Ann Arbor/1/86), intranasally live attenuated, cold-adapted type A/Texas/1/85 (H1N1) reassortant virus. In all volunteers, cells spontaneously secreting IgA, IgG IgM antibodies specific to virus detected in peripheral blood on days 6–13 after immunization, measured sera external secretions (saliva nasal lavage). Following...
Abstract The expression of secretory component (SC), the epithelial receptor for polymeric Ig, was enhanced by addition human rIFN-gamma or rIL-4, as revealed binding radiolabeled polymeric, J chain-containing IgA anti-SC antisera to colonic adenocarcinoma cell line HT-29. In combination, these cytokines exhibited a synergistic effect, and potentiating effect IL-4 inhibitable polyclonal anti-IL-4 antisera. Because (pIgA) HT-29 cells inhibited unlabeled pIgA reagent, but not IgG, monomeric...
Aberrant O-glycosylation of serum immunoglobulin A1 (IgA1) represents a heritable pathogenic defect in IgA nephropathy, the most common form glomerulonephritis worldwide, but specific genetic factors involved its determination are not known. We performed quantitative GWAS for levels galactose-deficient IgA1 (Gd-IgA1) 2,633 subjects European and East Asian ancestry discovered two genome-wide significant loci, C1GALT1 (rs13226913, P = 3.2 x 10-11) C1GALT1C1 (rs5910940, 2.7 10-8). These genes...