A5031880117- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Allergic Rhinitis and Sensitization
- Eosinophilic Esophagitis
- Mast cells and histamine
- Contact Dermatitis and Allergies
- Eosinophilic Disorders and Syndromes
- Glioma Diagnosis and Treatment
- Parasites and Host Interactions
- Respiratory viral infections research
- Parasitic Diseases Research and Treatment
- Parasite Biology and Host Interactions
- HIV Research and Treatment
- Olfactory and Sensory Function Studies
- Neuroblastoma Research and Treatments
- Veterinary Pharmacology and Anesthesia
- Animal testing and alternatives
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- Gastrointestinal Tumor Research and Treatment
- Histone Deacetylase Inhibitors Research
- Phagocytosis and Immune Regulation
- T-cell and B-cell Immunology
- Anesthesia and Sedative Agents
- Anesthesia and Neurotoxicity Research
Leidos (United States)
2022-2023
Leidos Biomedical Research Inc. (United States)
2022-2023
Frederick National Laboratory for Cancer Research
2023
Yale University
2021
Uniformed Services University of the Health Sciences
2011-2018
Colorado State University
2018
Iowa State University
2015
Baylor College of Medicine
2014
National Institutes of Health
2009-2011
National Institute of Allergy and Infectious Diseases
2009-2011
The inflammatory response to respiratory virus infection can be complex and refractory standard therapy. Lactobacilli, when targeted the epithelium, are highly effective at suppressing virus-induced inflammation protecting against lethal disease. Specifically, wild-type mice primed via intranasal inoculation with live or heat-inactivated Lactobacillus plantarum reuteri were completely protected virulent rodent pathogen, pneumonia of mice; significant protection (60% survival) persisted for...
Leading hypotheses to explain helminth-mediated protection against autoimmunity postulate that type 2 or regulatory immune responses induced by helminth infections in the host limit pathogenic Th1-driven autoimmune responses. We tested these investigating whether infection with filarial nematode Litomosoides sigmodontis prevents diabetes onset IL-4-deficient NOD mice and depletion absence of T cells, IL-10, TGF-β alters protection. In contrast IL-4-competent mice, failed develop a shift...
The development of an effective vaccine to protect against HIV acquisition will be greatly bolstered by in-depth understanding the innate and adaptive responses vaccination. We report here that efficacy DNA/ALVAC/gp120/alum vaccines, based on V2-specific antibodies mediating apoptosis infected cells (V2-ADCC), is complemented efferocytosis, a cyclic AMP (cAMP)-dependent antiphlogistic engulfment apoptotic CD14
Background Chronic helminth infections induce a Th2 immune shift and establish an immunoregulatory milieu. As both of these responses can suppress Th1 immunity, which is necessary for control Mycobacterium tuberculosis (MTB) infection, we hypothesized that chronic may exacerbate the course MTB. Methodology/Principal Findings Co-infection studies were conducted in cotton rats as they are natural host filarial nematode Litomosoides sigmodontis excellent model human Immunogical responses,...
We investigated the impact of monocytes, NK cells, and CD8 + T-cells in primary HTLV-1 infection by depleting cell subsets exposing macaques to either wild type (HTLV-1 WT ) or p12KO mutant unable infect replete animals due a single point mutation orf-I that inhibits its expression. The orf- I encoded p8/p12 proteins counteract cytotoxic favor viral DNA persistence monocytes. Double depletion alone accelerated seroconversion all exposed . In contrast, infectivity was fully restored only when...
Allergic responses are the result of activation mast cells and basophils, subsequent release vasoactive proinflammatory mediators. Exposure to an allergen in a sensitized individual can clinical symptoms that vary from minor erythema life threatening anaphylaxis. In laboratory, various animal models have been developed understand mechanisms driving allergic responses. Herein, we describe detailed method for measuring changes vascular permeability quantify localized The local anaphylaxis...
Intestinal intussusception (ISS) commonly causes intestinal obstruction in children. One mechanism that has been proposed to cause ISS is inflammation-induced alteration of motility. We investigated whether innate inflammatory factors or altered motility required for induction by LPS. compared rates among BALB/c and C57BL/6 mice, mice lacking lymphocytes depleted phagocytes, with defects the Toll-like receptor 4 (TLR4) signaling pathway following administration LPS Ca 2+ analog MnCl 2 . At 6...
In this study, we evaluated the effect chronic helminth infection has on allergic disease in mice previously sensitized to OVA. Ten weeks of with Litomosoides sigmodontis reduced immunological markers type I hypersensitivity, including OVA-specific IgE, basophil activation, and mast cell degranulation. Despite these reductions, there was no protection against immediate clinical hypersensitivity following intradermal OVA challenge. However, late-phase ear swelling, due III significantly...
Allergic responses are the result of activation mast cells and basophils, subsequent release vasoactive proinflammatory mediators. Exposure to an allergen in a sensitized individual can clinical symptoms that vary from minor erythema life threatening anaphylaxis. In laboratory, various animal models have been developed understand mechanisms driving allergic responses. Herein, we describe detailed method for measuring changes vascular permeability quantify localized The local anaphylaxis...
Long-acting subcutaneous lenacapavir (LEN), a first-in-class HIV capsid inhibitor approved by the US FDA for treatment of multidrug-resistant HIV-1 with twice yearly dosing, is under investigation pre-exposure prophylaxis (PrEP). We previously derived simian-tropic clone (stHIV-A19) that encodes an and replicates to high titres in pigtail macaques (PTM), resulting nonhuman primate model well-suited evaluating LEN PrEP vivo.
Rush desensitization can provide short-term tolerance to individuals who are allergic certain medications in instances where other therapeutic interventions limited. While rush (DS) is typically successful preventing adverse type I hypersensitivity reactions, the mechanism of protection remains unknown. Given rise prevalence displaying multiple allergies, understanding impact DS on "bystander" allergens, or additional allergens which an individual sensitized, could help inform clinical...
Anesthesia in rhesus macaques is required for many procedures. Although ketamine the backbone of most anesthetic protocols, tolerance to drug can develop, resulting need higher doses provide sufficient restraint. Combination with other drugs, such as α-agonists, be ketamine-sparing, providing restraint at lower doses. In addition, because α-agonists are reversible, recovery from anesthesia has potential much shorter. We hypothesized that use a low dose high dexmedetomidine, an α2 receptor...
Abstract Mirdametinib is a MEK inhibitor with reported CSF penetration in pre-clinical models. Murine studies ERK phosphorylation inhibition brain tissue at 1.15 nM (0.73 ng/mL) and tumor cell lines 0.33–0.59 (0.16-0.28 ng/mL). A phase II clinical trial evaluating mirdametinib for neurofibromatosis type 1-related plexiform neurofibromas 42% partial response rate mean plasma exposure (AUC0-12h) of 443 h*ng/mL. This study determined the pharmacokinetic (PK) profile non-human primate (NHP)...
Abstract Panobinostat is an HDAC inhibitor with dose-dependent pre-clinical activity against pediatric glioma. Reported pharmacokinetic (PK) profiles of panobinostat, including CSF penetration, have varied. We hypothesized that this variability resulted from the utilization differing panobinostat formulations. For comparison to USP grade powdered formulation PK profile formerly reported (Rodgers, et al., Cancer Chemother Pharmacol. 2020 Apr;85(4):827-830) a plasma and study was conducted...