A5030388445- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Biochemical and Molecular Research
- Insect symbiosis and bacterial influences
- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Synthesis and Biological Evaluation
- Antimicrobial Peptides and Activities
- Particle Detector Development and Performance
- Invertebrate Immune Response Mechanisms
- Viral Infections and Immunology Research
- Studies on Chitinases and Chitosanases
- Antibiotics Pharmacokinetics and Efficacy
- Parasitic Infections and Diagnostics
- Parasites and Host Interactions
- Scientific Computing and Data Management
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Parasitic Diseases Research and Treatment
- Liver Disease Diagnosis and Treatment
- Antibiotic Resistance in Bacteria
- Minerals Flotation and Separation Techniques
- Signaling Pathways in Disease
Universidad de Las Tunas
2025
Simon Fraser University
2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2010-2020
National University of General San Martín
2008-2020
University of New Mexico
2018-2020
Universidad Nacional de San Martín
2019
Instituto Tecnológico de Chascomús
2001-2018
Technological University of Pereira
2018
Favaloro University
2017
Instituto de Neurología Cognitiva
2017
Whole-genome sequencing of the protozoan pathogen Trypanosoma cruzi revealed that diploid genome contains a predicted 22,570 proteins encoded by genes, which 12,570 represent allelic pairs. Over 50% consists repeated sequences, such as retrotransposons and genes for large families surface molecules, include trans-sialidases, mucins, gp63s, novel family (>1300 copies) mucin-associated protein (MASP) genes. Analyses T. cruzi, brucei , Leishmania major (Tritryp) genomes imply differences...
ABSTRACT As part of a Brucella abortus 2308 genome project carried out in our laboratory, we identified, cloned, and sequenced genomic DNA fragment containing locus ( virB ) highly homologous to bacterial type IV secretion systems. The B. is collinear arrangement 13 open reading frames (ORFs). Between virB1 virB2 downstream ORF12, two degenerated, palindromic repeat sequences characteristic intergenic regions were found. Gene reporter studies demonstrated that the constitutes an operon...
The availability of Roundup Ready (RR) varieties soybean has increased the use glyphosate for weed control in Argentina. Glyphosate [(N-phosphonomethyl)glycine] is employed eradication previous crop vegetation and during growing cycle. Its action effective, low environmental impact been reported so far. No residues have observed soil or water, either its metabolite, AMPA (aminomethylphosphonic acid). objective this work was to monitor plants grains field crops Santa Fe Province, Five sites...
Trypanosoma cruzi has a complex mucin gene family of 500 members with hypervariable regions expressed preferentially in vertebrate associated stages the parasite. In this work, novel mucin-type is reported, composed two groups genes organized independent tandems and having very short open reading frames. The structures deduced proteins share N C termini but differ central regions. One group repeats consensus Lys-Asn-Thr7-Ser-Thr3-Ser(Ser/Lys)-Ala-Pro other Thr-rich sequence type...
gp63 is a highly abundant glycosylphosphatidylinositol (GPI)-anchored membrane protein expressed predominantly in the promastigote but also amastigote stage of Leishmania species. In spp., has been implicated number steps establishment infection. Here we demonstrate that Trypanosoma cruzi, etiological agent Chagas' disease, family genes composed multiple groups. Two these groups, Tcgp63-I and -II, are present as high-copy-number genes. The genomic organization mRNA expression pattern were...
The eosinophil cationic protein/RNase 3 and the skin-derived RNase 7 are two human antimicrobial RNases involved in host innate immunity. Both belong to A superfamily share a high cationicity common structural architecture. However, they present significant divergence at their primary structures, displaying either number of Arg or Lys residues, respectively. Previous comparative studies with membrane model revealed distinct mechanisms action for lipid bilayer disruption. We have now compared...
Climate change is a global environmental threat, directly affecting biodiversity. Terrestrial gastropods are particularly susceptible to alterations in temperature and humidity have develop morph-physiological behavioural adaptations this regard. Shell colour polymorphism its potential implication for thermoresistance constitute an unexplored field Neotropical land snails. The variation shell luminance characterized the threatened endemic Eastern Cuban tree snails Polymita picta muscarum...
Campylobacter fetus subspecies venerealis is the causative agent of bovine genital campylobacteriosis, asymptomatic in bulls disease spread to female cattle causing extensive reproductive loss. The microbiological and molecular differentiation C. subsp. from extremely difficult. This study describes analysis available AZUL-94 strain genome (~75–80%) identify elements exclusively found strains as potential diagnostic targets characterisation virulence genes. Eighty Kb genomic sequence (22...
Analysis of expressed sequence tags (ESTs) constitutes a useful approach for gene identification that, in the case human pathogens, might result new targets chemotherapy and vaccine development. As part Trypanosoma cruzi genome project, we have partially sequenced 5' ends 1, 949 clones to generate ESTs. The were randomly selected from normalized CL Brener epimastigote cDNA library. A total 14.6% homologous previously identified T. genes, while 18.4% had significant matches genes other...
Addition of sialic acid residues in the human pathogen Trypanosoma cruzi glycoconjugates is mediated by a trans‐sialidase and not CMP‐sialic acid:glycoconjugate sialyltransferase. Incubation with N ‐[ galactose ‐ 14 C]acetyllactosamine O ‐linked oligosaccharides, N‐linked glycopeptides (both obtained from fetuin) or sialyllactose showed that last three compounds were donors to first one. Moreover, N‐ O‐linked oligosaccharides asialofetuin asialomucin, respectively, served as acceptors units....
trans-sialidase is a unique sialidase in that, instead of hydrolizing sialic acid, it preferentially transfers the monosaccharide to terminal beta-galactose glycoproteins and glycolipids. This enzyme, originally identified Trypanosoma cruzi, belongs large family proteins. Some members lack enzymatic activity. No function has been yet assigned them. In this work, gene copy number possible inactive trans -sialidase was studied. It shown that genes encoding are not few, but rather, present same...
Abstract Human antimicrobial RN ases, which belong to the vertebrate ase A superfamily and are secreted upon infection, display a wide spectrum of antipathogen activities. In this work, we examined antifungal activity eosinophil 3 skin‐derived 7, two proteins expressed by innate cell types that directly involved in host defense against fungal infection. Candida albicans has been selected as suitable working model for testing activities toward eukaryotic pathogen. We explored distinct levels...
Mucins are highly O-glycosylated molecules which in mammalian cells accomplish essential functions, like cytoprotection and cell-cell interactions. In the protozoan parasite Trypanosoma cruzi, mucin-related glycoproteins have been shown to play a relevant role interaction with invasion of host cells. We previously reported family mucin-like genes T. cruzi whose overall structure resembled that mucin genes. now analyzed relationship between these proteins. A monoclonal antibody specific for...
Abstract A new enzyme immunoassay (EIA), the Dia Kit Bio-Chagas assay (Gador S.A.), is potentially useful for detecting antibodies to Trypanosoma cruzi in diagnosis of infected individuals and screening blood banks. The EIA carried out on test strips plastic backing covered with a nitrocellulose membrane which mixture recombinant T. antigens 1, 2, shed acute-phase antigen, 13, 30 has been applied as horizontal line. line human IgG included monitor procedure. exhibited homogeneity adsorption...
TcTASV-C is a protein family of about 15 members that expressed only in the trypomastigote stage Trypanosoma cruzi. We have previously shown located at parasite surface and secreted to medium. Here we report expression different genes occurs simultaneously while some parasite-associated products are found both fractions, others different. Secreted mainly shedded through extracellular vesicles, which they an abundant constituent, despite its scarce on culture-derived trypomastigotes. In...