A5102956710- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Neurobiology and Insect Physiology Research
- Neuropeptides and Animal Physiology
- Venomous Animal Envenomation and Studies
- Amphibian and Reptile Biology
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immune Response and Inflammation
- Aquaculture disease management and microbiota
Nanjing University of Chinese Medicine
2019-2023
University of Macau
2019
Queen's University Belfast
2014
Although the physicochemical properties of antimicrobial peptides (AMPs) and anticancer (ACPs) are very similar, it remains unclear which specific parameter(s) ACPs confer major activity. By answering how to construct a short AMP/ACP that could easily be synthesized in most cost effective way plus conferring maximum effect is important scientific breakthrough development protein/peptide drugs. In this study, an 18-amino-acids peptide, AcrAP1 (named AP1-Z1), was used as template....
The non disulphide-bridged peptides (NDBPs) of scorpion venoms are attracting increased interest due to their structural heterogeneity and broad spectrum biological activities.Here, two novel peptides, named AcrAP1 AcrAP2, have been identified in the lyophilised venom Arabian scorpion, Androctonus crassicauda, through "shotgun" molecular cloning biosynthetic precursor-encoding cDNAs.The respective mature predicted from these cloned cDNAs, were subsequently isolated same sample using reverse...
Amphibians are a natural source of abundant antimicrobial peptides and thus have been widely investigated for isolation such biomolecules. Many new peptide families discovered from amphibians. In this study, novel named Limnonectes fujianensis Brevinvin (LFB) has identified in the skin secretion Fujian large headed frog, fujianensis. The cDNA sequence was cloned library predicted mature through MS/MS fragmentation sequencing reverse phase HPLC fractions on same sample. LFB to be an...
In the development and study of antimicrobial peptides (AMPs), researchers have kept a watchful eye on from brevinin family because their extensive activities anticancer potency. this study, novel peptide was isolated skin secretions Wuyi torrent frog, Amolops wuyiensis (A. wuyiensisi), named B1AW (FLPLLAGLAANFLPQIICKIARKC). displayed anti-bacterial activity against Gram-positive bacteria Staphylococcus aureus (S. aureus), methicillin-resistant (MRSA), Enterococcus faecalis (E. faecalis)....
The first amphibian skin antimicrobial peptide (AMP) to be identified was named bombinin, reflecting its origin from the of European yellow-bellied toad (Bombina variegata). Bombinins and their related peptides, bombinin Hs, were subsequently reported other bombinid toads. Molecular cloning bombinin-encoding cDNAs found that bombinins Hs coencoded on same precursor proteins. Here, we report molecular two novel a secretion-derived cDNA library B. variegata whose open-reading frames each...
Kunitz-like trypsin inhibitors are one of the most noteworthy research objects owing to their significance in pharmacological studies, including anticarcinogenic activity, obesity regulation and anticoagulation. In current study, a novel inhibitor, Kunitzin-AH, was isolated from skin secretion Amolops hainanensis. The peptide displayed modest inhibitory activity with inhibitor constant (Ki) value 1.18 ± 0.08 µM without inducing damage healthy horse erythrocytes. Then, series shortened...
Tryptophyllins are a diverse family of amphibian peptides originally found in extracts phyllomedusine frog skin by chemical means. Their biological activities remain obscure. Here we describe the isolation and preliminary pharmacological characterization novel type 2 tryptophyllin, named AcT-2, from secretion red-eyed leaf frog, Agalychnis callidryas . The peptide was initially identified during smooth muscle screening HPLC fractions unique primary structure—GMRPPWF-NH —was established both...
One of the most widespread and abundant families pharmacologically active peptides in amphibian defensive skin secretions is bradykinins related peptides. Despite retaining certain primary structural attributes that assign them to this peptide family, are unique among they exhibit a wide range variations, post-translational modifications and/or N-terminal or C-terminal extensions. Initially it was believed their high degree heterogeneity reflective random gene mutations within species, but...
The defensive skin secretions of many amphibians are a rich source bradykinins and bradykinin-related peptides (BRPs). Members this peptide group also common components reptile arthropod venoms due to their multiple biological functions that include induction pain, effects on smooth muscle types, lowering systemic blood pressure. While most BRPs bradykinin receptor agonists, some have curiously been found be exquisite antagonists, such as the maximakinin gene-related peptide, kinestatin—a...
The skin secretions of Neotropical phyllomedusine leaf frogs have proven to be a rich source biologically active peptides, including antimicrobials. major families antimicrobial peptides (AMPs) reported are the dermaseptins and phylloseptins minor dermatoxins, phylloxins, plasticins, distinctins, medusins. Here, we report novel AMP 10 amino acid residues (LRPAILVRIKamide), named balteatide, from secretion wild Peruvian purple-sided frogs, Phyllomedusa baltea . Balteatide was found exhibit...
In recent years, there has been growing scientific interest in balancing the bioactivity and cytotoxicity of antimicrobial peptides (AMPs) for more comprehensive research application. this study, we focused on t-DPH1, a representative member dermaseptin family, as template. We synthesized five analogues were synthesised to assess impact three conserved features family (1. tryptophan residue at 3rd position, 2. consensus motif mid-region, 3. C-terminal amidation) t-DPH1 their potential reduce...