The number of people living with HIV/AIDS (PLHIV), new HIV infections, and deaths due to in China were estimated. These data provided evidences for the analysis on current epidemic China, development AIDS prevention control strategies public health education. However, whether estimation results could be fully used practice depends appropriate interpretation. Since differences methods produce estimates each year, it is not suitable directly use different years conclude trends. 2018 indicated...

Abstract In this study, a novel scorpion venom‐derived peptide named Gonearrestide was identified in an in‐house constructed venom library through combination of high‐throughput NGS transcriptome and MS/MS proteome platform. total, 238 peptides were discovered from two species; 22 selected for further study after battery functional prediction analysis. Following series bioinformatics analysis alongside with vitro biological screenings, found to be highly potent anticancer which acts on broad...

Abstract Osteopontin ( OPN ) has been shown to promote colorectal cancer CRC progression; however, the mechanism of ‐induced progression is largely unknown. In this study, we found that overexpression led enhanced anchorage‐independent growth, cell migration and invasion in KRAS gene mutant cells but a lesser extent wild‐type cells. also induced PI 3K signalling, expression Snail Matrix metallopeptidase 9 MMP 9), suppressed E‐cadherin human specimens, high‐level significantly predicted...

// Bin Li 1 , Cian M. McCrudden 2 Hiu Fung Yuen 3 Xinping Xi Peng Lyu Kwok Wah Chan 4 Shu Dong Zhang 5 Hang Fai Faculty of Health Sciences, University Macau, Avenida de Universidade, Taipa, Macau SAR School Pharmacy, Queen’s Belfast, United Kingdom Institute Molecular and Cell Biology, A*STAR, Singapore Department Pathology, Hong Kong, Kong Northern Ireland Centre for Stratified Medicine, Biomedical Sciences Research Institute, Ulster, Londonderry, Correspondence to: Kwok, email:...

Soft-tissue sarcomas (STS) emerges as formidable challenges in clinics due to the complex genetic heterogeneity, high rates of local recurrence and metastasis. Exploring specific targets biomarkers would benefit prognosis treatment STS. Here, we identified RCC1, a guanine-nucleotide exchange factor for Ran, an oncogene potential intervention target Bioinformatics analysis indicated that RCC1 is highly expressed correlated with poor Functional studies showed knockdown significantly inhibited...

Amphibians are a natural source of abundant antimicrobial peptides and thus have been widely investigated for isolation such biomolecules. Many new peptide families discovered from amphibians. In this study, novel named Limnonectes fujianensis Brevinvin (LFB) has identified in the skin secretion Fujian large headed frog, fujianensis. The cDNA sequence was cloned library predicted mature through MS/MS fragmentation sequencing reverse phase HPLC fractions on same sample. LFB to be an...

Osteopontin (OPN) plays an important role in cancer progression, however its prognostic significance and downstream factors are largely elusive.In this study, we have shown that expression of OPN was significantly higher bladder specimens with T-stage or tumor grades.In addition, a high level associated poorer survival two independent patient cohorts totaling 389 patients available data.We further identified Matrix metallopeptidase 9 (MMP9) S100 calcium-binding protein A8 (S100A8) were both...

Abstract A simple and feasible pH meter–based immunoassay is reported for detection of C-reactive protein (CRP) using glucose oxidase (GOD)–conjugated dendrimer loaded with platinum nanozyme. Initially, nanozymes were into the dendrimers through an in situ synthetic method. Then, GOD monoclonal anti-CRP antibody a high molar ratio covalently conjugated onto carboxylated via typical carbodiimide coupling. The immunoreaction was carried out competitive mode CRP-coated microplate. Along...

Amphibian venom-derived peptides have high potential in the field of anticancer drug discovery. We isolated a novel Bowman-Birk proteinase inhibitor (BBI)-type peptide from skin secretion Pelophylax esculentus (PE) named PE-BBI, and evaluated its bio-functions anti-cancer activity vitro. PE-BBI is heptadecapeptide with C-terminal amidation. The mRNA sequence primary structure were identified using RT-PCR LC/MS, respectively. A trypsin inhibitory assay was used to characterize serine...

Colorectal cancer (CRC) is a major public health concern and the second leading cause of cancer-related deaths worldwide. However, challenges remain in deploying effective screening strategies for early-stage CRC. This study aimed to evaluate effectiveness fecal-based syndecan-2 (SDC2) methylation test detection colorectal lesions We retrospectively collected data on participants who underwent fecal SDC2 testing from January 1, 2019, May 30, 2023. Patients with positive results were...

Tryptophyllins are a diverse family of amphibian peptides originally found in extracts phyllomedusine frog skin by chemical means. Their biological activities remain obscure. Here we describe the isolation and preliminary pharmacological characterization novel type 2 tryptophyllin, named AcT-2, from secretion red-eyed leaf frog, Agalychnis callidryas . The peptide was initially identified during smooth muscle screening HPLC fractions unique primary structure—GMRPPWF-NH —was established both...

One of the most widespread and abundant families pharmacologically active peptides in amphibian defensive skin secretions is bradykinins related peptides. Despite retaining certain primary structural attributes that assign them to this peptide family, are unique among they exhibit a wide range variations, post-translational modifications and/or N-terminal or C-terminal extensions. Initially it was believed their high degree heterogeneity reflective random gene mutations within species, but...

Abstract This communication describes a simple sensitive one‐step potentiometric aptasensing method for quantitative detection of referenced therapeutic biomarker (programmed death‐ligand 1, PD−L1). The aptasensor is constructed by modifying PD−L1‐specific aptamer on Ti 3 C 2 ‐MXene nanosheets‐functionalized electrode. Introduction PD−L1 induces the specific reaction between and aptamer, thereby resulting in change spatial structures. surface electric potential modified electrode shifted...

An electrochemical immunoassay was designed to detect prostate-specific antigen<italic>via</italic>pyrophosphatase-hydrolysed Cu(<sc>ii</sc>)-coordinated pyrophosphate ion with the capture of releasing Cu(<sc>ii</sc>) ion.

Target identification is an essential step in drug discovery. It facilitates understanding of action and potential toxicities offers opportunities to repurpose candidates. HP-1, a potent EGFRL858R/T790M (epidermal growth factor receptor) mutant inhibitor, was developed by the group effort treat acquired resistance nonsmall cell lung cancer (NSCLC), but its cellular off-targets were not identified. An activity-based probe, HJ-1, created followed chemical proteomics bioimaging studies. A total...

Objective: To understand the basic characteristics of death cases, analyze trends in AIDS patients and risk factors China provide evidence for development prevention control strategy. Methods: The data were collected from national information system HIV/AIDS. cases phase used. number mortality described, Cox Proportion Hazards Regression Model was constructed to assess hazard ratios (HR) independent variables. Results: By end 2019, a total 582 472 including 168 391 deaths, had been reported...

Objective: To understand how social and cultural factors influence sexual perceptions, practices, HIV transmission among men who have sex with at selected sites in China. Methods: Qualitative methodology was used face to face, semi-structured, in-depth interviews conducted from April 2013 October 2015 Sichuan, Jiangxi, Henan, Heilongjiang provinces Chongqing municipality of Results: A total 184 participated the interviews. Forty-eight originated Henan Province, 12, 50, 47, 27 Heilongjiang,...