A5100365220- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Force Microscopy Techniques and Applications
- Biosensors and Analytical Detection
- Extracellular vesicles in disease
- Molecular Junctions and Nanostructures
- Lipid Membrane Structure and Behavior
- Plasmonic and Surface Plasmon Research
- Advanced Biosensing Techniques and Applications
- Cellular Mechanics and Interactions
- GaN-based semiconductor devices and materials
- Supramolecular Self-Assembly in Materials
- Electrochemical Analysis and Applications
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Atomic and Molecular Physics
- DNA and Nucleic Acid Chemistry
- Ga2O3 and related materials
- MicroRNA in disease regulation
- Gold and Silver Nanoparticles Synthesis and Applications
- Semiconductor Quantum Structures and Devices
- Spectroscopy and Laser Applications
- Graphene research and applications
- Protein Degradation and Inhibitors
- Antenna Design and Analysis
Chinese Academy of Sciences
2016-2025
Shanghai Institute of Applied Physics
2015-2025
University of Chinese Academy of Sciences
2022-2025
Hunan University
2020-2025
Shanghai Advanced Research Institute
2019-2024
Shanghai Stomatological Hospital
2022-2024
Fudan University
2022-2024
Hunan University of Traditional Chinese Medicine
2018-2024
Nanjing University of Chinese Medicine
2018-2023
University of Macau
2018-2023
Abstract Direct imaging of single‐molecule and its movement is fundamental importance in biology, but challenging. Herein, aided by the nanoconfinement effect resultant high reaction activity within metal–organic frameworks (MOFs), designed Ru(bpy) 3 2+ embedded MOF complex (RuMOFs) exhibits bright electrochemiluminescence (ECL) emission permitting high‐quality ECL events at single molecule level. By labeling individual proteins living cells with RuMOFs, distribution membrane...
Abstract: Exosomes, small extracellular vesicles derived from cells, are known to carry important bioactive molecules such as proteins, nucleic acids, and lipids. These components play crucial roles in cell signaling, immune response, tumor metastasis, making exosomes potential diagnostic biomarkers for various diseases. However, current methods detecting face scientific challenges including low sensitivity, poor specificity, complicated procedures, high costs. It is essential surmount these...
Abstract Encapsulating individual mammalian cells with biomimetic materials holds potential in ex vivo cell culture and engineering. However, current methodologies often present tradeoffs between homogeneity, stability, compatibility. Here, inspired by bacteria that use proteins stably anchored on their outer membranes to nucleate biofilm growth, we develop a single‐cell encapsulation strategy using DNA framework structure as nucleator (DFN) initiate the growth of hydrogels under...
The DNA origami technology holds great promise for the assembly of nanoscopic technological devices and studies biochemical reactions at single-molecule level. For these, it is essential to establish well controlled attachment functional materials predefined sites on nanostructures reliable measurements versatile applications. However, two-sided nature scaffold has shown limitations in this regard. We hypothesized that holes commonly used two-dimensional designs are large enough passage...
Abstract Acupuncture has historically been practiced to treat medical disorders by mechanically stimulating specific acupoints with fine needles. Despite its well-documented efficacy, biological basis remains largely elusive. In this study, we found that mechanical stimulation at the acupoint of Yanglingquan (GB34) promoted autophagic clearance α-synuclein (α-syn), a well known aggregation-prone protein closely related Parkinson’s disease (PD), in substantia nigra par compacta (SNpc) brain...
Tumor cells metastasizing through the bloodstream or lymphatic systems must withstand acute shear stress (ASS). Autophagy is a cell survival mechanism that functions in response to stressful conditions, but also contributes death apoptosis. We predicted compensation pathway autophagy exists tumor subjected mechanical stress. found ASS promoted autophagosome (AP) accumulation and induced release of extracellular nanovesicles (EVs) containing components. Furthermore, we autophagic vesicles...
Patterned assembly of multivalent antibody complexes using DNA nanostructure templates holds the potential for advancing studies cellular signaling and smart theranostic applications. However, evaluating heterogeneity in protein conjugation efficiency at distinct sites on remains challenging. Here, we utilize atomic force microscopy to measure coupling antibodies various positions two-dimensional rectangular origami frameworks single-molecule level, generating spatial maps binding...
A novel single-molecular fluorescent probe was developed for spatiotemporal matrix metalloproteinase-2 (MMP-2) and caspase-3 imaging with distinct fluorescence signals. Due to the multi-Förster resonance energy transfer (FRET) processes, could respond MMP-2 independently high signal-to-noise ratio. Moreover, overexpression of in cancer cell lines cisplatin induced apoptosis were imaged emissions. Because independent process imaging, meet demands precise disease diagnosis theranostic...
In addition to being an important object in theoretical and experimental studies enzymology, RNase A also plays role the development of many kinds diseases by regulating various physiological or pathological processes, including cell growth, proliferation, differentiation, invasion. Thus, it can be used as a useful biomarker for disease theranostics. Here, simple, sensitive, low-cost assay was constructed combining fluorogenic substrate with reduced graphene oxide (rGO). The method detection...
A myriad of DNA origami nanostructures have been demonstrated in various intriguing applications. In pursuit facile yet high-yield synthesis, the mechanisms underlying folding need to be resolved. Here, we visualize processes several multidomain structures under ambient annealing conditions solution using atomic force microscopy with submolecular resolution. We reveal coexistence diverse transitional that might result same prescribed products. Based on experimental observations and...
Mechanistic understanding of how living systems sense, transduce, and respond to mechanical cues has important implications in development, physiology, therapy. Here, the authors use an integrated atomic force microscope (AFM) brightfield/epifluorescent platform precisely simulate single cells or groups under physiological conditions, real time, concomitantly measuring single-cell autophagic response its transmission neighboring cells. Dual-color fluorescence monitoring cellular reveals...
Oligomerization of cellular membrane receptors plays crucial roles in activating intracellular downstream signaling cascades for controlling behaviors physiological and pathological processes. However, the reversible controllable regulation a user-defined manner remains challenging. Herein, we developed versatile DNA nanorobot (nR) with installed aptamers hairpin structures to reversibly controllably regulate cell migration. This was achieved by dimerization de-dimerization...
Molecular dynamics simulation was carried out to investigate the stick-slip on an atomic scale by using embedded atom method potential for Ni–Al. The analyses of dynamic features atoms in sliding block clearly show that elastic deformation surface layers is main cause phenomenon, which consistent with macroscopic stick-slip. results also indicate phonons are emitted during stick-slip, and a commensurate fit between contacting surfaces not significant friction.