Mastoparan is a typical cationic and amphipathic tetradecapeptide found in wasp venom exhibits potent biological activities. Yet, compared with other insect-derived peptides, such as melittin from the bee venom, this family have been underrated. Herein, we evaluated activities of mastoparan-C (MP-C), which was identified European Hornet (Vespa crabro), rationally designed two analogues (a skeleton-based cyclization by cysteine residues an N-terminal extension via tat-linked) for enhancing...

The dermaseptin peptides, mainly derived from the skin secretions of Hylidae frogs, belong to a superfamily antimicrobial peptides and exhibit diverse anticancer activities with low cytotoxicity. Here, we reported novel peptide, South American orange-legged leaf Pithecopus (Phyllomedusa) hypochondrialis, processing shortest peptide length, namely Dermaseptin-PH. complementary DNA (cDNA) encoding biosynthetic precursor Dermaseptin-PH was initially identified by rapid amplification cDNA ends...

The dermaseptin antimicrobial peptide family contains members of 27-34 amino acids in length that have been predominantly isolated from the skins/skin secretions phyllomedusine leaf frogs. By use a degenerate primer Rapid amplification cDNA ends (RACE) PCR designed to common conserved domain within 5'-untranslated regions previously-characterized encoding cDNAs, two novel this family, named dermaseptin-PD-1 and dermaseptin-PD-2, were identified skin secretion frog, Pachymedusa dacnicolor....

Antimicrobial peptides (AMPs) in the skin secretions of amphibians are fundamental components a unique defense system that has evolved to protect these hosts from microbial invasion. Medusins constitute recently-discovered AMP family phyllomedusine leaf frog and exhibit highly-conserved structural characteristics. Here, we report novel medusin, medusin-PT, secretion Tarsier Leaf Frog, Phyllomedusa tarsius. The mature peptide was initially identified its cloned biosynthetic precursor-encoding...

Temporin is an antimicrobial peptide (AMP) family discovered in the skin secretion of ranid frog that has become a promising alternative for conventional antibiotic therapy. Herein, novel temporin peptide, Temporin-PF (TPF), was successfully identified from Pelophylax fukienensis. It exhibited potent activity against Gram-positive bacteria, but no effect on Gram-negative bacteria. Additionally, TPF aggregation effects different solutions. Three analogs were further designed to study...

The main functions of the abundant polypeptide toxins present in scorpion venoms are debilitation arthropod prey or defence against predators. These effects achieved mainly through blocking an array ion channel types within membranes excitable cells. However, while these channel-blocking tightly-folded by multiple disulphide bridges between cysteine residues, there additional groups peptides that devoid residues. non-disulphide bridged subject much research interest, and among exhibit...

Abstract In this study, a novel scorpion venom‐derived peptide named Gonearrestide was identified in an in‐house constructed venom library through combination of high‐throughput NGS transcriptome and MS/MS proteome platform. total, 238 peptides were discovered from two species; 22 selected for further study after battery functional prediction analysis. Following series bioinformatics analysis alongside with vitro biological screenings, found to be highly potent anticancer which acts on broad...

Abstract Osteopontin ( OPN ) has been shown to promote colorectal cancer CRC progression; however, the mechanism of ‐induced progression is largely unknown. In this study, we found that overexpression led enhanced anchorage‐independent growth, cell migration and invasion in KRAS gene mutant cells but a lesser extent wild‐type cells. also induced PI 3K signalling, expression Snail Matrix metallopeptidase 9 MMP 9), suppressed E‐cadherin human specimens, high‐level significantly predicted...

Antimicrobial peptides belonging to the phylloseptin family are mainly found in phyllomedusine frogs. These not only possess potent antimicrobial activity but exhibit low toxicity against eukaryotic cells. Therefore, they considered as promising drug candidates for a number of diseases. In recent study, was correlated with conserved structures and cationic amphiphilic characteristics members this peptide family. A precursor discovered here skin secretion Phyllomedusa tarsius mature validated...

// Bin Li 1 , Cian M. McCrudden 2 Hiu Fung Yuen 3 Xinping Xi Peng Lyu Kwok Wah Chan 4 Shu Dong Zhang 5 Hang Fai Faculty of Health Sciences, University Macau, Avenida de Universidade, Taipa, Macau SAR School Pharmacy, Queen’s Belfast, United Kingdom Institute Molecular and Cell Biology, A*STAR, Singapore Department Pathology, Hong Kong, Kong Northern Ireland Centre for Stratified Medicine, Biomedical Sciences Research Institute, Ulster, Londonderry, Correspondence to: Kwok, email:...

Antimicrobial peptides are a promising resource for developing novel antibiotic and even anticancer drugs. Here, 28-mer polypeptide, Ranatuerin-2PLx (R2PLx), was identified from lyophilised skin secretions. The chemically synthetic replicates exhibited moderate broadspectrum antimicrobial effect against various microorganisms including methicillin-resistant Staphylococcus aureus (MRSA, minimal inhibitory concentration = 256 µM). In addition, R2PLx found to inhibit the proliferation of...

Antimicrobial peptides (AMPs) from the skin secretions of amphibians are now considered as a potential alternative to conventional antibiotics. Phylloseptins family AMPs identified in Phyllomedusinae tree frogs which exhibit highly conserved structural characteristics. This study examines structure-activity relationship newly discovered phylloseptin, Phylloseptin-PHa (PSPHa) Pithecopus hypochondrialis.PSPHa and modified analogs were produced by solid phase synthesis purified reverse-phase...

Many antimicrobial peptides (AMPs) have been identified from the skin secretion of frog Hylarana guentheri (H.guentheri), including Temporin, Brevinin-1, and Brevinin-2. In this study, an peptide named Brevinin-1GHa was for first time by using ‘shotgun’ cloning. The primary structure also confirmed through mass spectral analysis purified reversed-phase high-performance liquid chromatography (RP-HPLC). There a Rana-box (CKISKKC) in C-terminal Brevinin-1GHa, which formed intra-disulfide...

Abstract Host-defense antimicrobial peptides (AMPs) from amphibians are usually considered as one of the most promising next-generation antibiotics because their excellent properties and low cytotoxicity. In present study, novel Brevinin-1 type peptide, Brevinin-1GHd, was isolated characterized skin secretion frog, Hylarana guentheri. Brevinin-1GHd found to possess a wide range activity through penetrating bacterial membrane within short time while showing hemolysis at bactericidal...

Antimicrobial peptides (AMPs) are promising therapeutic alternatives compared to conventional antibiotics for the treatment of drug-resistant bacterial infections. However, application overwhelming majority AMPs is limited because high toxicity and manufacturing costs. Amphibian skin secretion has been proven be a source discovery development novel AMPs. Herein, we discovered AMP from Odorrana schmackeri, designed analogues by altering key factors, including conformation, net charge...

Public health is confronting the threat caused by antibiotic resistance and this means new antibacterial strategies must be developed urgently. Antimicrobial peptides (AMPs) have been considered as promising therapeutic candidates against infection in post-antibiotic era. In paper, we dismissed significance of "Rana box" natural nigrocin-HL identified from skin secretion Hylarana latouchii comparing its activity with nigrocin-HLD without motif. By substituting sequence an amidated...

Dermaseptins belonging to a large family of cationic membrane-disruption antimicrobial peptides display extensive antibacterial and antiproliferative activities depending on coil-to-helix transition the specific structural parameters. Herein, novel dermaseptin peptide named Der-PS4 was discovered from skin secretion waxy monkey tree frog, Phyllomedusa sauvagii. The complementary DNA (cDNA)-encoding precursor obtained relying “shotgun” cloning, afterwards, mature amino acid sequence...

Lung cancer is the major cause of deaths worldwide, and it has highest incidence mortality rate any among men women in China. The first-line therapy for lung treatment platinum-based chemotherapy drugs such as cisplatin. However, application present chemotherapies limited by severe side effects, which stimulates discovery new with anti-tumor mechanisms fewer effects. Beneficially, many antimicrobial peptides (AMPs) from frog skin have been reported to exhibit potent anti-cancer activities...

Antimicrobial peptides from amphibian skin secretion display remarkable broad-spectrum antimicrobial activity and are thus promising for the discovery of new antibiotics. In this study, we report a novel peptide belonging to phylloseptin family peptides, purple-sided leaf frog, Phyllomedusa baltea, which was named Phylloseptin-PBa. Degenerate primers complementary putative signal sites frog precursor-encoding cDNAs were designed interrogate secretion-derived cDNA library frog. Subsequently,...

The skin secretions of the subfamily Phyllomedusinae have long been known to contain a number compounds with antimicrobial potential. Herein, biosynthetic dermaseptin-precursor cDNA was obtained from Phyllomedusa sauvagii secretion-derived library, and thereafter, presence mature peptide, namely dermaseptin-PS3 (DPS3), confirmed by LC–MS/MS. Moreover, this naturally occurring peptide utilized design two analogues, K5, 17-DPS3 (introducing lysine residues at positions 5 17 replace acidic...

Peptides derived from amphibian skin secretion are promising drug prototypes for combating widespread infection. In this study, a novel peptide belonging to the phylloseptin family of antimicrobial peptides was isolated Phyllomedusa camba, namely phylloseptin-PC (PSN-PC). The biosynthetic precursor obtained by molecular cloning and mature sequence confirmed through tandem mass spectrometry (MS/MS) fragmentation sequencing in secretion. synthetic replicate exhibited broad spectrum activity...

Antimicrobial peptides (AMPs) are considered as a promising agent to overcome the drug-resistance of bacteria. Large numbers AMPs have been identified from skin secretion Rana pipiens, including brevinins, ranatuerins, temporins and esculentins. In this study, cDNA precursor broad-spectrum antimicrobial peptide, ranatuerin-2Pb, was cloned identified. Additionally, two truncated analogues, RPa RPb, were synthesised investigate structure-activity relationship ranatuerin-2Pb. lost activity...