Abstract The human gastrointestinal tract is populated with a diverse microbial community. vast genetic and metabolic potential of the gut microbiome underpins its ubiquity in nearly every aspect biology, including health maintenance, development, aging, disease. advent new sequencing technologies culture-independent methods has allowed researchers to move beyond correlative studies toward mechanistic explorations shed light on microbiome–host interactions. Evidence unveiled bidirectional...

Human umbilical cord mesenchymal stem cell-derived small extracellular vesicle (hUC-MSCs-sEVs) therapy has shown promising results to treat diabetes mellitus in preclinical studies. However, the dosage of MSCs-sEVs animal studies, up 10 mg/kg, was considered high and may be impractical for future clinical application. This study aims investigate efficacy low-dose hUC-MSCs-sEVs treatment on human skeletal muscle cells (HSkMCs) type 2 (T2DM) rats. Treatment with 100 μg/mL 48 h showed no...

Melanoma, the most aggressive human skin tumor, has a very short survival time, and there are currently no effective treatments. Alterations in cell metabolism, such as enhanced aerobic glycolysis, have been identified hallmarks of cancer cells. In present study, bioinformatics studies using online databases revealed that FOXO3a expression was lower melanoma tissues compared with normal nevus. Additionally, Kaplan‑Meier analysis showed high predicted an improved prognosis for patients...

Zerumbone (ZER), a potential anticancer agent for breast cancer treatment, has limited clinical applications due to its poor water solubility and targeting ability. The of ZER been partly overcome by loading into nanostructured lipid carriers (ZER-NLC). However, the lack selectivity is still concern in settings. To these limitations, ZER-NLC was co-loaded with an active-targeting known as superparamagnetic iron oxide nanoparticles (SPION) that may direct tumor site external magnetic field....

Background and Aims EGFR activation PKM2 expression are instrumental in tumorigenesis. regulates functions a subcellular compartment-dependent manner promotes gene transcription tumor growth. In addition, is upregulated EGFR-induced pathways glioma malignancies. However, we found that could also regulate the activity of EGF/EGFR signaling pathway gastric cancer cells. We aimed to define biological mechanisms for regulating cell motility invasion. Methods employed stable transfection with...

Lung cancer is the major cause of deaths worldwide, and it has highest incidence mortality rate any among men women in China. The first-line therapy for lung treatment platinum-based chemotherapy drugs such as cisplatin. However, application present chemotherapies limited by severe side effects, which stimulates discovery new with anti-tumor mechanisms fewer effects. Beneficially, many antimicrobial peptides (AMPs) from frog skin have been reported to exhibit potent anti-cancer activities...

Background/Aims: The tumor-suppressive functions of interferon regulatory factor 6 (IRF6) in some tumors have been preliminarily established, but its pathogenesis and underlying molecular mechanisms breast cancer, the most common malignancy women, remains poorly understood. Methods: Pairs typical cancer cell lines (high- low-aggressive) addition to 27 tissue samples 31 non-cancerous tissues were used investigate expression level IRF6 Lentivirus-mediated gain-of-function studies, short...

There is a need to shift the paradigm of cancer therapeutic approach. The severe adverse side effects, drug resistanceand unaffordable price plagued with chemotherapeutic drugs has spurred development “dirty drug”. Natural products,specifically phytochemicals, have gained much attention due their ability target multiple interconnected pathways.Resveratrol (RSV), stilbenes found in red wine, one phytochemicals that exhibits various pharmacologicaltherapeutic effects including cancer. In this...

Abstract Objective Despite the demonstrated anti‐melanogenic and UV protective effects of Zerumbone (ZER) in vitro, there is a lack clinical trials that have been done to assess these properties. The primary objective this study was effectiveness ZER lightening skin tone human participants with single‐blind approach. Methods Twenty‐six were randomly assigned two groups investigate application location (left or right volar forearm) for placebo creams. Both creams topically administered...

Abstract: Clustered Regions of Interspersed Palindromic Repeat (CRISPR)-based techniques have been utilized in various research areas, including agriculture, biotechnology, and medicine. With the use a short sequence guide RNA CRISPR-associated (Cas) protein, this technique allows for robust, site-specific manipulation genome, aiding researchers making important biomedical discoveries scientific advancements. In review, we explored applications CRISPR/Cas systems field parasitology...

To investigate the effect of total ginsenoside ginseng stems and leaves (TGSL) on pharmacokinetics aspirin in rats. Sprague-Dawley rats were randomly divided into two groups (n = 6), a combined group that received TGSL (625 mg/kg body weight) (10 by gavage, an gavage. The concentration salicylic acid, important metabolite aspirin, was determined high-performance liquid chromatography supernatant from blood obtained orbital sinus at various time points to examine aspirin. results showed Tmax...

To improve a capillary electrophoresis method used for the rapid and simultaneous separation determination of rutin, chlorogenic acid quercetin. The column without coating (50μm×56cm, length: 47.5cm) was used, solution 12mM sodium tetraborate pentahydrate (pH 9.0) containing 15% methanol taken as running buffer temperature 25 _. linear relationship, recover rate repeatability quercetin were better when they detected at wave length 350nm. we should be accurate suitable quality control...

Zerumbone (ZER), a potential anticancer agent for breast cancer treatment, has limited clinical applications due to its poor water solubility and targeting ability. The of ZER been partly overcome by loading into nanostructured lipid carriers (ZER-NLC). However, the lack selectivity is still concern in settings. To these limitations, ZER-NLC was co-loaded with an active-targeting known as superparamagnetic iron oxide nanoparticles (SPION) that may direct tumor site external magnetic field....

To investigate the pharmacokinetics (PK) and pharmacodynamics (PD) of index components Zisu Fang preparations additionally analysis anti-dementia drug system characteristics. A PK-PD-drug interaction (DI) method was applied to determine characteristics in vivo. In PK study, maximum plasma concentration, area under concentration–time curve, mean residence time were higher memory-deficit model group than control group. This suggested that had an affinity for state dementia this model. PD at...

Objective To observe the effect of polydatin on nerve cell apoptosis and influence caspase-8 FLIP protein expression after ischemia reperfusion injury. Methods Thirty rats were randomly divided into sham operation group, model group group. The middle cerebral artery occlusion (MCAO) focal was established by method thread embolism. Rats subjected to adaptive feeding for first 7 days then recieved treatment another 10 days. injury at 14th day. received 15 mg/kg polydatin, with same...

IJS Oncology is a peer-reviewed, international, open access journal seeking to publish in the broad field of oncology, including surgical, medical and radiation oncology. The Journal aims basic science, educational, clinical translational research articles. We aim provide rapid submission decision times whilst maintaining high quality peer-review process.The science practice oncology rapidly developing field. It has become increasingly interdisciplinary through recognition efficacy...

Abstract Background: This study was aimed to investigate the effect of Scutellarin on apoptosis human colorectal cancer SW480 cells and azoxymethane (AOM)/dextran sulfate sodium (DSS) mouse, clarify its mechanism. Methods: 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT) cell migration assay were performed detect viability proliferation that treated with different concentrations Scutellarin. Hoechst33342 Staining determine apoptosis, detecting Caspase-3 Caspase-9...

Abstract Background: Jiawei Ling Gui Zhu Gan Decoction (JW-LZD) is based on Linggui Zhugan (LZD) and adds eight traditional Chinese medicines: Codonopsis pilosula, Astragalus membranaceus, Yam, Epimedium, Morinda officinalis, Tangerine Peel, Pinellia ternata Coix Seed , which can be used for the treatment of obesity in clinical. However, pharmacological mechanisms JW-LZD remain unclear. Methods: In this study, we determined molecular targets mechanismsinvolved through network analysis,...