A5101528520- Force Microscopy Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Nanopore and Nanochannel Transport Studies
- Protein Structure and Dynamics
- Insect symbiosis and bacterial influences
- Parasitic Diseases Research and Treatment
- Material Dynamics and Properties
- Lipid Membrane Structure and Behavior
- Mechanical and Optical Resonators
- RNA and protein synthesis mechanisms
- Theoretical and Computational Physics
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Molecular Junctions and Nanostructures
- Petroleum Processing and Analysis
- Enhanced Oil Recovery Techniques
- Synthesis and Characterization of Heterocyclic Compounds
- Hydrocarbon exploration and reservoir analysis
- Nematode management and characterization studies
- Synthesis and biological activity
- Spectroscopy and Quantum Chemical Studies
- stochastic dynamics and bifurcation
Banaras Hindu University
2015-2024
Louisiana State University
2021-2024
Graphic Era University
2023
Indian Institute of Technology BHU
2023
Novel (United States)
2023
University of Jammu
2017
Cairn Research (United Kingdom)
2016
New England Biolabs (United States)
2005-2015
Maharshi Dayanand University
2013
Max Planck Institute for the Physics of Complex Systems
2005-2007
Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions people in the developing world. We have sequenced approximately 90 megabase (Mb) genome human filarial parasite Brugia malayi predict 11,500 protein coding genes 71 Mb robustly assembled sequence. Comparative analysis with free-living, model nematode Caenorhabditis elegans revealed that, despite these having maintained little conservation local synteny during 350 million years evolution, they...
Complete genome DNA sequence and analysis is presented for Wolbachia, the obligate alpha-proteobacterial endosymbiont required fertility survival of human filarial parasitic nematode Brugia malayi. Although, quantitatively, even more degraded than those closely related Rickettsia species, Wolbachia has retained intact metabolic pathways. The ability to provide riboflavin, flavin adenine dinucleotide, heme, nucleotides likely be Wolbachia's principal contribution mutualistic relationship,...
Abstract Background Wolbachia ( w Bm) is an obligate endosymbiotic bacterium of Brugia malayi , a parasitic filarial nematode humans and one the causative agents lymphatic filariasis. There pressing need for new drugs against parasites, such as B. . As Bm required development fertility, targeting promising approach. However, lifecycle neither nor can be maintained in vitro To facilitate selection potential drug targets we computationally ranked genome based on confidence that particular gene...
Background Wolbachia are among the most abundant symbiotic microbes on earth; they present in about 66% of all insect species, some spiders, mites and crustaceans, filarial nematode species. Infected nematodes, including many pathogens medical veterinary importance, depend for proper development survival. The mechanisms behind this interdependence not understood. Interestingly, a minority species examined to date naturally Wolbachia-free. Methodology/Principal Findings We used 454...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTProtein-Modulated DNA Electron TransferScott R. Rajski, Sanjay Kumar, Richard J. Roberts, and Jacqueline K. BartonView Author Information The Division of Chemistry Chemical Engineering California Institute Technology Pasadena, 91125 New England Biolabs, 32 Tozer Road Beverly, Massachusetts 01915 Cite this: Am. Chem. Soc. 1999, 121, 23, 5615–5616Publication Date (Web):May 25, 1999Publication History Received11 January 1999Published online25...
We report results from the first genome-wide application of a rational drug target selection methodology to metazoan pathogen genome, completed draft sequence Brugia malayi, parasitic nematode responsible for human lymphatic filariasis. More than 1.5 billion people worldwide are at risk contracting filariasis and onchocerciasis, related filarial disease. Drug treatments have not changed significantly in over 20 years, with resistance rising, there is an urgent need development new...
A very large monotonic variation of asphaltenes and viscosity has been measured by downhole fluid analysis (DFA) in crude oils five-stacked sandstone reservoirs the northern part Barmer Basin, northwest India, undergoing active biodegradation; each five-layered sand bodies shows overlaying gradients with depth providing replicate validation measurements. Fluid data from four wells across field that are uniform formation. The oil upper half column exhibits an equilibrium distribution matching...
Lateral gene transfer events between bacteria and animals highlight an avenue for evolutionary genomic loss/gain of function. Herein, we report functional lateral in animal parasitic nematodes. Members the Nematoda are heme auxotrophs, lacking ability to synthesize heme; however, human filarial parasite Brugia malayi has acquired a bacterial encoding ferrochelatase (BmFeCH), terminal step biosynthesis. BmFeCH, encoded by 9-exon gene, is mitochondrial-targeted, based on enzyme assays,...
4i-Thio-2i-deoxycytidine was synthesized as a 5iprotected phosphoramidite compatible with solid phase DNA synthesis.When incorporated the target cytosine (C*) in GC*GC recognition sequence for methyltransferase M.HhaI, methyl transfer strongly inhibited.In contrast, these same oligonucleotides were normal substrates cognate restriction endonuclease R.HhaI and its isoschizomer R.HinP1I.M.HhaI able to bind both 4i-thio-modified unmodified equivalent extents under equilibrium...
Human filarial parasites infect an estimated 120 million people in 80 countries worldwide causing blindness and the gross disfigurement of limbs genitals. An understanding RNA-mediated regulatory pathways these may open new avenues for treatment. Toward this goal, small RNAs from Brugia malayi adult females, males microfilariae were cloned deep-sequencing. From ∼30 sequencing reads, 145 miRNAs identified B. genome. Some microRNAs validated using p19 RNA binding protein qPCR. segregate into...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPurification, crystallization, and preliminary x-ray diffraction analysis of an M.HhaI-AdoMet complexSanjay Kumar, Xiaodong Cheng, James W. Pflugrath, Richard J. RobertsCite this: Biochemistry 1992, 31, 36, 8648–8653Publication Date (Print):September 15, 1992Publication History Published online1 May 2002Published inissue 15 September 1992https://pubs.acs.org/doi/10.1021/bi00151a035https://doi.org/10.1021/bi00151a035research-articleACS...
A statistical mechanical description of flexible and semiflexible polymer chains in a poor solvent is developed the constant force distance ensembles. We predict existence many intermediate states at low temperatures stabilized by force. unified response to pulling compressing forces has been obtained ensemble. show signature crossover length which increases linearly with chain length. Below this length, critical unfolding decreases temperature, while above, it temperature. For stiff chains,...
Wolbachia endosymbionts are widespread in arthropods and generally considered reproductive parasites, inducing various phenotypes including cytoplasmic incompatibility, parthenogenesis, feminization male killing, which serve to promote their spread through populations. In contrast, infecting filarial nematodes that cause human diseases, elephantiasis river blindness, obligate mutualists. DNA purification methods for efficient genomic sequencing of these unculturable bacteria have proven...
A simple model of DNA based on two interacting polymers has been used to study the unzipping a double stranded subjected periodic force. We propose dynamical transition, where without changing physiological condition, it is possible bring from zipped/unzipped state new dynamic (hysteretic) by varying frequency applied Our studies reveal that area hystersis loop grows with same exponents as isotropic spin systems. These are amenable verification in force spectroscopic experiments.
Phosphoglycerate mutases catalyze the interconversion of 2- and 3-phosphoglycerate in glycolytic gluconeogenic pathways. They exist two unrelated forms that are either cofactor (2,3-diphosphoglycerate)-dependent or cofactor-independent. The enzymes have no similarity amino acid sequence, tertiary structure, catalytic mechanism. Certain organisms including vertebrates only cofactor-dependent form, whereas other can possess independent form both. Caenorhabditis elegans has been predicted to...
We consider a model of self-avoiding walk on lattice with on-site repulsion and an attraction for every vertex the visited surface to study force-induced desorption linear polymer chain adsorbed attractive use exact enumeration technique analyzing how critical force $f_c(T)$ depends temperature. The curve gives boundary separating phase from desorbed phase. Our results show that in two dimensions where is line increases monotonically as temperature lowered becomes almost constant at very low...
The nature of the globule-coil transition surface-confined polymers has been an issue debate. Here this 2D collapse is studied through a partially directed lattice model. In general case with positive bending stiffness ($\ensuremath{\Delta}>0$), first order; it becomes second order only in limiting zero ($\ensuremath{\Delta}\ensuremath{\equiv}0$). These analytical results are confirmed by Monte Carlo simulations. We also suggest some possible future experiments.