A5019997139- Cancer Genomics and Diagnostics
- Parasite Biology and Host Interactions
- Insect symbiosis and bacterial influences
- Parasitic Diseases Research and Treatment
- Parasites and Host Interactions
- Helminth infection and control
- Glioma Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Identification and Quantification in Food
- Aquatic Invertebrate Ecology and Behavior
- RNA modifications and cancer
- Fish Biology and Ecology Studies
- MicroRNA in disease regulation
- Neuroendocrine Tumor Research Advances
- Nematode management and characterization studies
- Genetic factors in colorectal cancer
- Molecular Biology Techniques and Applications
- Hematopoietic Stem Cell Transplantation
- Meningioma and schwannoma management
- Neurofibromatosis and Schwannoma Cases
- Lung Cancer Treatments and Mutations
- Tumors and Oncological Cases
- Genomics and Rare Diseases
- Hedgehog Signaling Pathway Studies
- RNA Research and Splicing
Danaher (United States)
2025
Invitae (United States)
2022-2024
Washington University in St. Louis
2012-2022
James S. McDonnell Foundation
2016-2020
Food borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four which recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among other NTDs due to its broad and significant impact on both human animal health, Fasciola sp., also considered major pathogens domesticated ruminants. Here we present a reference genome sequence common liver fluke, hepatica isolated from sheep, complementing previously reported isolate cattle. A...
Background Wolbachia are among the most abundant symbiotic microbes on earth; they present in about 66% of all insect species, some spiders, mites and crustaceans, filarial nematode species. Infected nematodes, including many pathogens medical veterinary importance, depend for proper development survival. The mechanisms behind this interdependence not understood. Interestingly, a minority species examined to date naturally Wolbachia-free. Methodology/Principal Findings We used 454...
Helminth.net (http://www.helminth.net) is the new moniker for a collection of databases: Nematode.net and Trematode.net. Within this we provide services resources parasitic roundworms (nematodes) flatworms (trematodes), collectively known as helminths. For over decade have provided studying nematodes via our veteran site (http://nematode.net). In article, (i) an update on expansions that hosts omics data from 84 species provides advanced search tools to broad scientific community so can be...
GLI1 encodes a transcription factor that targets cell cycle regulators affecting stem proliferation. gene fusions were initially described in pericytomas with t[7;12] translocation and more recently gastric plexiform fibromyxomas gastroblastomas. This study describes the clinicopathologic, immunohistochemical, molecular features of three intestinal-based neoplasms harboring fusions. We studied unique mesenchymal small bowel tumors. Paraffin embedded tumor tissues from these cases 62...
Enasidenib (ENA) is an inhibitor of isocitrate dehydrogenase 2 (IDH2) approved for the treatment patients with IDH2-mutant relapsed/refractory acute myeloid leukemia (AML). In this phase 2/1b Beat AML substudy, we applied a risk-adapted approach to assess efficacy ENA monotherapy aged ≥60 years newly diagnosed in whom genomic profiling demonstrated that mutant IDH2 was dominant leukemic clone. Patients did not induce complete remission (CR) or CR incomplete blood count recovery (CRi)...
Relapsed high-risk neuroblastomas (NBLs) are enriched for targetable mutations in ALK and RAS-MAPK pathways, yet the prognostic effect of these aberrations relevance subclonal at diagnosis remain undefined. We describe spectrum clinical significance clonal pathogenic alterations NBL. developed a focused NBL sequencing panel including ALK, NRAS, KRAS, HRAS, BRAF, PTPN11, TP53, ATRX genes ultra-deep applied this assay to 242 pretherapy tumors from patients enrolled on phase III trial...
Many species of filarial nematodes depend on Wolbachia endobacteria to carry out their life cycle. Other are naturally Wolbachia-free. The biological mechanisms underpinning Wolbachia-dependence and independence in not known. Previous studies have indicated that an impact mitochondrial gene expression, which may suggest a role energy metabolism. If can supplement host metabolism, reduced function infected account for Wolbachia-dependence. also strong influence evolution due vertical...
Abstract Meningiomas are common in adults (~35% of brain tumors) but rare children, where they exhibit unique clinical, pathological and molecular features compared to adult counterparts. Thus, data generated from cohorts may be imperfectly suited guiding diagnostic, prognostic treatment decisions for children. We studied 50 meningioma patients ≤18 years with available clinical address the need obtained pediatric setting. As previously described, we noted a slight bias toward male higher...
Abstract Motivation The acquisition of somatic mutations in hematopoietic stem and progenitor cells with resultant clonal expansion, termed hematopoiesis (CH), is associated increased risk hematologic malignancies other adverse outcomes. CH generally present at low allelic fractions, but expansion additional leads to cancers a small proportion individuals. With high depth sensitivity sequencing, can be detected most adults its trajectory mapped over time. However, accurate variant calling...
Abstract The bovine lungworm, Dictyocaulus viviparus (order Strongylida), is an important parasite of livestock that causes substantial economic and production losses worldwide. Here we report the draft genome, variome developmental transcriptome D. . genome (161 Mb) smaller than those related bursate nematodes encodes fewer proteins (14,171 total). In first genome-wide assessment genomic variation in any parasitic nematode, found a high degree sequence variability predicted to be involved...
Abstract Background Paragonimus spp. (lung flukes) are among the most injurious foodborne helminths, infecting ∼23 million people and subjecting ∼292 to infection risk. Paragonimiasis is acquired from infected undercooked crustaceans primarily affects lungs but often causes lesions elsewhere including brain. The disease easily mistaken for tuberculosis owing similar pulmonary symptoms, accordingly, diagnostics in demand. Results We assembled, annotated, compared draft genomes of 4 prevalent...
Background Paragonimiasis is a food-borne trematode infection acquired by eating raw or undercooked crustaceans. It major public health problem in the far East, but it also occurs South Asia, Africa, and Americas. Paragonimus worms cause chronic lung disease with cough, fever hemoptysis that can be confused tuberculosis other non-parasitic diseases. Treatment straightforward, diagnosis often delayed due to lack of reliable parasitological serodiagnostic tests. Hence, purpose this study was...
Abstract Glioblastoma (GBM) is the most common primary malignant brain tumor in adults. Prior studies examining mutational landscape of GBM revealed recurrent alterations genes that regulate same growth control pathways. To this regard, ~ 40% harbor EGFR alterations, whereas BRAF variants are rare. Existing data suggests gain-of-function mutations these mutually exclusive. This study was designed to explore clinical, pathological, and molecular differences between EGFR- BRAF- mutated GBM. We...
Most filarial species in the genus Onchocerca depend on Wolbachia endobacteria to successfully carry out their life cycle. O. flexuosa is a Wolbachia-free species, but its genome contains Wolbachia-like sequences presumably obtained from via horizontal gene transfer. Proteogenomic studies have shown that many of these are expressed adult worms. Six were chosen for further study based sequence conservation with genes, length predicted open reading frames, and expression at RNA and/or protein...
FGFR2 is recurrently amplified in 5% of gastric cancers and 1%–4% breast cancers; however, this molecular alteration has never been reported a primary colorectal cancer specimen. Preclinical studies indicate that several FGFR tyrosine-kinase inhibitors (TKIs), such as AZD4547, have vitro activity against the -amplified cell line, NCI-H716. The efficacy these currently under investigation clinical trials for cancer. Thus, better characterizing tumors amplification could identify subset...
Most filarial parasites in the subfamilies Onchocercinae and Dirofilariinae depend on Wolbachia endobacteria to successfully carry out their life cycle. Recently published data indicate that few Wolbachia-free species these were infected distant past have subsequently shed endosymbionts. We used an integrated transcriptomic proteomic analysis of Onchocerca flexuosa explore molecular mechanisms allow worms this survive without a bacterial partner. Roche/454 sequencing adult transcriptome...