A5073684130- Ultrasound and Hyperthermia Applications
- Ultrasound Imaging and Elastography
- Photoacoustic and Ultrasonic Imaging
- Ultrasound and Cavitation Phenomena
- Microfluidic and Bio-sensing Technologies
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Microbial Inactivation Methods
- Infrared Thermography in Medicine
- Thermography and Photoacoustic Techniques
- Cardiac Arrhythmias and Treatments
- Ultrasonics and Acoustic Wave Propagation
- Blood properties and coagulation
- Collagen: Extraction and Characterization
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Pluripotent Stem Cells Research
- Ultrasound in Clinical Applications
- Electrical and Bioimpedance Tomography
- Cardiac electrophysiology and arrhythmias
- Heterotopic Ossification and Related Conditions
- Liver Disease Diagnosis and Treatment
- Bone Tissue Engineering Materials
- Bone fractures and treatments
- Elasticity and Material Modeling
- Atrial Fibrillation Management and Outcomes
University of Michigan–Ann Arbor
2016-2025
Michigan United
2013-2022
Creative Commons
2017
Bellingham Technical College
2015
Washington University in St. Louis
2012
Cardiovascular Research Center
2010
Morehouse School of Medicine
2010
University of Pittsburgh
2010
Cardiovascular Institute Hospital
2010
Uniformed Services University of the Health Sciences
2010
Abstract Transcranial focused ultrasound (US) has been demonstrated to stimulate neurons in animals and humans, but the mechanism of this effect is unknown. It hypothesized that US, a mechanical stimulus, may mediate cellular discharge by activating mechanosensitive ion channels embedded within membranes. To test hypothesis, we expressed potassium sodium (channels two-pore-domain family (K2P) including TREK-1, TREK-2, TRAAK; Na V 1.5) Xenopus oocyte system. Focused US (10 MHz, 0.3–4.9 W/cm 2...
This paper presents unique approaches to enable control and quantification of ultrasound-mediated cell membrane disruption, or sonoporation, at the single-cell level. Ultrasound excitation microbubbles that were targeted plasma HEK-293 cells generated spatially temporally controlled disruption with high repeatability. Using whole-cell patch clamp recording combined fluorescence microscopy, we obtained time-resolved measurements sonoporation quantified size resealing rate pores. We measured...
Fusobacterium nucleatum is a gram-negative anaerobe that prevalent in periodontal disease and infections of different parts the body. The organism has remarkable adherence properties, binding to partners ranging from eukaryotic prokaryotic cells extracellular macromolecules. Understanding its important for understanding pathogenesis F. nucleatum. In this study, novel adhesin, FadA (Fusobacterium adhesin A), was demonstrated bind surface proteins oral mucosal KB cells. composed 129 amino acid...
Ultrasonic spectrum analysis procedures have been developed to measure tissue morphologic features that are not well depicted with conventional ultrasonography. This article reviews some of the applications spectral techniques and provides an expanded theoretical framework showing how measured related spatial autocorrelation function descriptive microstructure. Explicit relationships obtained describe linear-regression parameters effective mean sizes, concentrations, relative mechanical...
The development of tools for patterning cocultures cells is a fundamental interest among cell biologists and tissue engineers. Although variety systems exist micropatterning cells, the methods used to generate micropatterns are often cumbersome difficult adapt engineering purposes. This study combines acoustic droplet ejection aqueous two-phase system exclusion introduce method in multiplexed arrays. new uses focused radiation pressure eject discrete droplets uniform size from surface...
This study investigates the feasibility of characterizing microstructures within a biological tissue by analyzing frequency spectrum photoacoustic signal from tissue. Hypotheses are derived theoretical analyses on relationships between dimensions/concentrations sources region-of-interest and linear model fitted to power spectra signals. The hypotheses validated, following procedures ultrasound analysis, simulations experiments with phantoms fabricated embedding polyethylene microspheres in...
Recent increases in the pressure output of diagnostic ultrasound scanners have led to an interest establishing thresholds for bioeffects many organs including lungs mammals. Damage may be mediated by inertial cavitation, yet there been no such direct observations vivo. To explore hypothesis cavitation-based from ultrasound, research has performed on damage rat exposed 4.0-MHz pulsed Doppler and color ultrasound. A 30-MHz active cavitation detection scheme complementing these studies provides...
The continued examination of potential biological effects ultrasound and their relationship to clinical practice is a key element in evaluating the safety diagnostic ultrasound. Periodically, American Institute Ultrasound Medicine (AIUM) sponsors conferences bringing experts together examine literature on bioeffects develop conclusions recommendations related most recent effort included whose origins were thermal or nonthermal, with separate evaluations for fetal In addition, due...
Nitro-oleic acid (OA-NO(2)) is a bioactive, nitric-oxide derived fatty with physiologically relevant vasculoprotective properties in vivo. OA-NO(2) exerts cell signaling actions as result of its strong electrophilic nature and mediates pleiotropic responses the vasculature.The present study sought to investigate protective role angiotensin (Ang) II-induced hypertension.We show that systemic administration results sustained reduction Ang hypertension mice significant blood pressure lowering...
Purpose: To evaluate the use of pulsed high-intensity focused ultrasound exposures to improve tissue plasminogen activator (tPA)-mediated thrombolysis in an vitro model. Materials and Methods: All experimental work was compliant with institutional guidelines HIPAA. Clots were formed by placing 1 mL human blood closed-off sections pediatric Penrose tubes. Four groups evaluated: control (nontreated) clots, clots treated only, tPA plus tPA. The (real or sham) followed incubations saline only....
Effective treatment of solid tumors requires homogeneous distribution anticancer drugs within the entire tumor volume to deliver lethal concentrations resistant cancer cells and tumor-initiating stem cells. However, penetration small molecular weight chemotherapeutic agents drug-loaded polymeric lipid particles into hypoxic necrotic regions remains a significant challenge. This article reports results pulsed ultrasound enhanced nanosized fluorescent MCF-7 breast spheroids (300-350 μm...
Mechanical forces are critical to modulate cell spreading, contractility, gene expression and even stem differentiation. Yet, existing tools that can apply controllable subcellular a large number of single cells simultaneously still limited. Here we report novel ultrasound tweezing cytometry utilizing pulses actuate functionalized lipid microbubbles covalently attached live exert mechanical in the pN - nN range. Ultrasonic excitation could elicit rapid sustained reactive intracellular...