A5059447982- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- Lipid metabolism and biosynthesis
- Liver Disease Diagnosis and Treatment
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Alzheimer's disease research and treatments
- Phagocytosis and Immune Regulation
- Cell death mechanisms and regulation
- Zebrafish Biomedical Research Applications
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- Diet, Metabolism, and Disease
- interferon and immune responses
- Cancer-related molecular mechanisms research
- Developmental Biology and Gene Regulation
- RNA regulation and disease
- Regulation of Appetite and Obesity
- Congenital heart defects research
- Peroxisome Proliferator-Activated Receptors
- Circular RNAs in diseases
- Aquaculture disease management and microbiota
- Epigenetics and DNA Methylation
- Retinoids in leukemia and cellular processes
National Yang Ming Chiao Tung University
2018-2024
National Taiwan Ocean University
2006-2018
Mekelle University
2018
Institute of Cellular and Organismic Biology, Academia Sinica
2005
Institute of Molecular Biology, Academia Sinica
2003-2004
Academia Sinica
2003-2004
Institute of Zoology
2003-2004
Mammalian liver fatty acid binding protein (L‐FABP) is a small cytosolic in various tissues including liver, intestine and kidney thought to play crucial role intracellular trafficking metabolism. To better understand its tissue‐specific regulation during zebrafish hepatogenesis, we isolated 5′‐flanking sequences of the L‐FABP gene used green fluorescent (GFP) transgenic strategy generate liver‐specific zebrafish. The 2.8‐kb sequence was sufficient direct GFP expression primordia, first...
Proteolytic processing of amyloid precursor protein (APP) C-terminal fragments (CTFs) by γ-secretase underlies the pathogenesis Alzheimer's disease (AD). An RNA interference screen using APP-CTF [99-residue CTF (C99)]- and Notch-specific interaction assays identified a unique ErbB2-centered signaling network that was predicted to preferentially govern proteostasis APP-C99. Consistently, significantly elevated levels ErbB2 were confirmed in hippocampus human AD brains. We then found...
MicroRNA-21 (miR-21) is one of the most frequently upregulated miRNAs in liver diseases such as nonalcoholic fatty disease (NAFLD) and hepatocellular carcinoma (HCC). However, mechanistic pathways that connect NAFLD HCC remain elusive. We developed a doxycycline (Dox)-inducible transgenic zebrafish model (LmiR21) which exhibited an upregulation miR-21 liver, turn induced full spectrum NAFLD, including steatosis, inflammation, fibrosis, HCC, LmiR21 fish. Diethylnitrosamine (DEN) treatment led...
Abstract Myostatin, a secreted growth and differentiation factor (GDF‐8) belongs to transforming (TGF‐β) superfamily that plays as negative regulator of skeletal muscle development growth. Recently, myostatin has been isolated from fish; however, its role in remains unknown. Here, we present the expression during effects knock‐down on various genes such regulatory transcription factors (MRFs), muscle‐specific proteins (MSP), insulin‐like (IGFs). The was found be maternal it starts one‐cell...
Activating transcription factor 4 (ATF4) is constitutively expressed in a variety of tissues, and regulates several pathological features associated with metabolic diseases such as non-alcoholic fatty liver (NAFLD) obesity. However, the role ATF4 animal model systems poorly understood. To investigate functions zebrafish, we conditionally proteins, using Tet-off transgenic system. We observed early-onset hyperlipidaemia steatosis zebrafish (ATs) without doxycycline treatment (ATs - Dox). Oil...
Nonalcoholic fatty liver disease (NAFLD) has been associated with the function and changes in expression levels of microRNAs (miRs). MiR-7 proven to play an important role many cellular processes; however, its functions context lipogenesis remain unknown. We applied microRNA-sponge (miR-SP) technology generated transgenic miR-7a-SP models (hC7aSP bC7aSP), which disrupted activities hepatic miR-7a induced early onset NAFLD nonalcoholic steatohepatitis (NASH) zebrafish. identified a novel...
Abstract Liver fatty acid binding protein (L‐FABP) is a small that thought to play an important role in the intracellular and trafficking of long chain acids liver. Expression gene encoding zebrafish liver regulated by 435‐bp distal region (−1944 −1510) L‐FABP promoter. The sequence sufficient for activation primordia (or bud) continues be active adult when positioned adjacent SV40 basal promoter linked directly green fluorescent protein. has two distinct regulatory elements, A −1623) B...
Colorectal cancer (CRC) is a leading cause of cancer-related mortality worldwide. Patients with inflammatory bowel disease (IBD) have high risk developing CRC. Inflammatory cytokines are regulated by complex gene networks and regulatory RNAs, especially microRNAs. MicroRNA-21 (miR-21) amongst the most frequently upregulated microRNAs in responses development. miR-21 has become target for genetic pharmacological regulation various diseases. However, association between inflammation...
Abstract Mammalian intestinal fatty acid‐binding protein (I‐FABP) is a small cytosolic and thought to play crucial role of intracellular acid trafficking metabolism in gut. To establish an vivo system for investigating its tissue‐specific regulation during zebrafish development, we isolated 5′‐flanking sequences the L‐FABP gene used transgenic strategy generate gut‐specific with green/red fluorescent intestine. The 4.5‐kb sequence I‐FABP was sufficient direct expression tube, first observed...
miR-27b has emerged as a regulatory hub in cholesterol and lipid metabolism, potential therapeutic target for treating atherosclerosis obesity. However, the impact of on levels vivo remains to be determined. Zebrafish lipids are normally stored triacylglycerols (TGs) their main storage sites visceral, intramuscular, subcutaneous depots, not blood vessels liver. In this study, we applied microRNA-sponge (miR-SP) technology generated zebrafish expressing transgenic miR-27b-SP (C27bSPs), which...
Abstract Intestinal‐type fatty acid binding protein (I‐FABP) plays an important role in the intracellular and trafficking of long chain acids intestine. The aim this study, therefore, was to elucidate regulation spatiotemporal expression I‐FABP gene during zebrafish larval development. We performed vivo reporter‐gene analysis by using a transient transgenic approach. Green fluorescent protein–reporter analyses revealed that proximal 192‐bp region promoter is sufficient direct...
The functions of anorexigenic neurons secreting proopiomelanocortin (POMC)/alpha-melanocyte-stimulating hormone (α-MSH) the melanocortin system in hypothalamus vertebrates are energy homeostasis, food intake, and body weight regulation. However, mechanisms remain elusive. This article reports on zebrafish that have been genetically engineered to produce α-MSH mutants, α-MSH-7aa α-MSH-8aa, selectively lacking 7 8 amino acids within region, but retaining most other normal...
Abstract Apoptosis is an essential part of normal embryonic development in vertebrates, and it involved sculpturing organs controlling cell populations. In previous studies, we identified two novel proteins, zfBLP1 zfMcl‐1a, which are similar to those the Bcl‐2 family as a group evolutionarily conserved proteins that regulate cellular anti‐apoptosis. To evaluate effect dysregulated hepatocyte apoptosis during zebrafish hepatogenesis, demonstrate transgenic overexpression either or zfMcl‐1a...