A5101579783- Lymphoma Diagnosis and Treatment
- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- Birth, Development, and Health
- Diet and metabolism studies
- Pancreatic function and diabetes
- NF-κB Signaling Pathways
- Chronic Lymphocytic Leukemia Research
- Renal and related cancers
- Endoplasmic Reticulum Stress and Disease
- Hedgehog Signaling Pathway Studies
- Escherichia coli research studies
- Peptidase Inhibition and Analysis
- Plant Molecular Biology Research
- Glycosylation and Glycoproteins Research
- Dermatology and Skin Diseases
- Pregnancy and preeclampsia studies
- Adipose Tissue and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Tryptophan and brain disorders
- Immune Cell Function and Interaction
- Gestational Diabetes Research and Management
- Plant Pathogenic Bacteria Studies
- Neurogenesis and neuroplasticity mechanisms
- Advanced Glycation End Products research
National Cheng Kung University
2015-2025
Taichung Veterans General Hospital
2021-2024
National Chung Hsing University
2023-2024
Kaohsiung Veterans General Hospital
2015-2023
Addenbrooke's Hospital
2023
University of Chinese Academy of Sciences
2023
National Yang Ming Chiao Tung University
2015-2021
Institute of Cellular and Organismic Biology, Academia Sinica
2015-2018
University of Cambridge
2011-2017
Queen Mary Hospital
2003-2015
Proteolytic processing of amyloid precursor protein (APP) C-terminal fragments (CTFs) by γ-secretase underlies the pathogenesis Alzheimer's disease (AD). An RNA interference screen using APP-CTF [99-residue CTF (C99)]- and Notch-specific interaction assays identified a unique ErbB2-centered signaling network that was predicted to preferentially govern proteostasis APP-C99. Consistently, significantly elevated levels ErbB2 were confirmed in hippocampus human AD brains. We then found...
Abstract Ca 2+ signaling is important to trigger the cell cycle progression, while it remains elusive in regulatory mechanisms. Here we show that store-operated entry (SOCE), mediated by interaction between STIM1 (an endoplasmic reticulum sensor) and Orai1 (a membrane pore structure), controls specific checkpoint of cycle. The fluctuating SOCE activity during progression universal different types, which upregulated G1/S transition downregulated from S G2/M transition. Pharmacological or...
Comorbidity exists between metabolic disorders and depressive syndrome with unclear mechanisms. To characterize the causal relationship, we adopted a 12-week high-fat diet (HFD) to induce disorder phenotypes in mice. Initially, identified an enhanced glutamatergic input nucleus accumbens of HFD Retrograde tracing chemogenetic inhibition showed that hyperactive ventral hippocampal afferents determined exhibition depression-like behavior Using lentiviral knockdown overexpression approaches,...
Abstract Microglial activation is implicated in the pathogenesis of Parkinson’s disease (PD). Although etiology PD remains unclear, age and male gender are known risk factors. By comparing microglia dopaminergic (DA) neurons substantia nigra (SN) female mice different ages, we found that degrees microglial DA neuron loss increased with both genders, but were more pronounced males, as peripheral lipopolysaccharide (LPS)-induced loss. A bilateral ovariectomy (OVX) eliminated female-associated...
ABSTRACT Colorectal cancer (CRC) has become one of the most arduous challenges in contemporary treatment. In nanomedicine, biomedical methodologies and nanomaterials are combined to develop novel effective treatments for illnesses, infections, cancer. The characteristics nanotechnology promising addressing urgent problems modern therapeutics, such as tumor recurrence, multidrug resistance, limited accessibility drugs tissue. Plant‐derived natural chemicals, termed phytochemicals, have...
Phalaenopsis orchids are popular floral crops, and development of new cultivars is economically important to floricultural industries worldwide. Analysis orchid genes could facilitate improvement. Bacterial artificial chromosome (BAC) end sequences (BESs) can provide the first glimpses into sequence composition a novel genome yield molecular markers for use in genetic mapping breeding.We used two BAC libraries (constructed using BamHI HindIII restriction enzymes) equestris generate pair-end...
Abstract Purpose: Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous group of aggressive lymphomas with the activated B-cell–like subtype characterized by constitutive NF-κB activation. Activating mutations CARD11 and inactivating A20 are frequent events in DLBCL. However, full extent genetic alterations pathway regulators their potential prognostic value DLBCL remain to be investigated. We investigated abnormalities CARD11, A20, ABIN-1/2/3 (the binding inhibitor NF-κB)...
Since the functional role(s) of angiotensin II (Ang II) type receptor (AT 2 R) in I diabetes is unknown, we hypothesized that AT R involved decreasing effects on kidneys. We induced with low-dose streptozotocin (STZ) both knockout RKO) and wild-type (WT) male mice aged 12 weeks followed them for 4 weeks. Three subgroups nondiabetic, diabetic, insulin-treated diabetic (Rx insulin implant) were studied. Systolic blood pressure (SBP), physiological parameters, glomerular filtration rate (GFR),...
Abstract Background Orchids are one of the most diversified angiosperms, but few genomic resources available for these non-model plants. In addition to ecological significance, Phalaenopsis has been considered as an economically important floriculture industry worldwide. We aimed use massively parallel 454 pyrosequencing a global characterization transcriptome. Results To maximize sequence diversity, we pooled RNA from 10 samples different tissues, various developmental stages, and biotic-...
The prevalence of diabetes is rapidly increasing worldwide and highly associated with the incidence depression. Pioglitazone, a Peroxisome proliferator-activated receptor gamma (PPAR-γ) agonist, widely used for treating patients type 2 diabetes. However, whether pioglitazone alleviates metabolic disorder-related depression astrocytic deficits remains unclear. Here we showed that 12 weeks high-fat diet (HFD) feeding (from 8- to 20-week-old) induced not only obesity insulin resistance, but...
The rate of neurogenesis is determined by 1) the number neural stem/progenitor cells (NSCs), 2) proliferation NSCs, 3) neuron lineage specification, and 4) survival newborn neurons. Aging lowers hippocampal neurogenesis, while exercise (Ex) increases this rate. However, it remains unclear which determinants are affected aging Ex. We characterized four in different age groups (3, 6, 9, 12, 21 months) mice that either received one month Ex training or remained sedentary. Bromodeoxyuridine...
Targeted next-generation sequencing based on PCR amplification involves pooling of hundreds to thousands primers, for preamplification and subsequent parallel single/multiplex PCR. It is often necessary allocate the set primers into subpools, a common issue being potential cross-hybridization. For smaller numbers pool division can be done manually using trial error minimize hybridization, but this becomes inefficient time consuming with increasing primer pairs. We developed PrimerPooler that...
The ideal characteristics of surface modification on the vascular graft for clinical application would be with excellent hemocompatibility, endothelialization capacity, and antirestenosis ability. Here, Fourier transform infrared spectroscopy (FTIR), enhanced Raman (SERS), atomic force microscopy (AFM), contact angle (θ) measurement, thermogravimetric analysis (TGA) were used to evaluate chemical mechanical properties collagen-gold nanocomposites (collagen+Au) 17.4, 43.5, 174 ppm Au...
We investigated whether overexpression of catalase (CAT) in renal proximal tubular cells (RPTCs) could prevent the programming hypertension and kidney disease offspring dams with maternal diabetes. Male nondiabetic diabetic from two transgenic (Tg) lines (Hoxb7-green fluorescent protein [GFP]-Tg [controls] Hoxb7/CAT-GFP-Tg, which overexpress CAT RPTCs) were studied prenatal period into adulthood. Nephrogenesis, systolic blood pressure, hyperfiltration, injury, reactive oxygen species (ROS)...
The clinical significance of STIM proteins and Orai Ca2+ channels in tumor progression has been demonstrated different types cancers. Podosomes are dynamic actin-rich cellular protrusions that facilitate cancer cell invasiveness by degrading extracellular matrix. Whether STIM1-dependent signaling facilitates invasion through affecting podosome formation remains unclear. Here we show the invasive fronts tissues overexpress STIM1, accompanied active store-operated entry (SOCE). Interfering...
Patients with type 2 diabetes mellitus have more risk to develop depression. Fluoxetine (FLX), a selective serotonin reuptake inhibitor (SSRI), is drug for mood and anxiety disorders. Previous studies showed that FLX could induce weight loss in non-depressed clinically overweight individuals. Although the anti-appetite effect of well-documented, its potential effects on metabolic abnormalities not been investigated. In this study, we want investigate whether be therapeutic against high fat...