Epidermal growth factor receptor (EGFR), a critical promotes lung cancer cell proliferation and survival. Knockdown of EGFR expression thus promise beating clinically. Functionalized gold nanoparticles may serve an effective vehicle carrying theranostic bio-active materials. Herein, physically were fabricated with biocompatible collagen to improve siRNA loading capacity treat cancer. Physic-chemical properties comprehensively characterized for the nanoparticle (C–Au), conjugation,...

This study compares the sensing performance of glassy carbon electrodes (GCE) modified with graphene (GR), multiwalled nanotubes (MWCNTs), and graphene-multiwalled nanotube (GR-MWCNT) composites for simultaneous detection ascorbic acid (AA), dopamine (DA), uric (UA). Among these electrodes, GR-MWCNT/GCE exhibited highest sensitivity lowest limit. Using differential pulse voltammetry (DPV), AA, DA, UA can be simultaneously detected at low potentials -0.032, 0.206, 0.34 V vs Ag/AgCl,...

Novel nanocomposites based on type I collagen (Col) containing a small amount (17.4, 43.5, and 174 ppm) of gold nanoparticles (AuNPs, approximately 5 nm) were prepared in this study. The pure Col Col-AuNP composites (Col-Au) characterized by the UV-Vis spectroscopy (UV-Vis), surface-enhanced raman (SERS) atomic force microscopy (AFM). interaction between AuNPs was confirmed infrared (IR) spectra. effect biocompatibility Col, evaluated proliferation reactive oxygen species (ROS) production...

Benzo[a]pyrene (BaP), a component of cooking oil fumes (COF), promotes lung cancer cell proliferation and survival via the induction inhibitor apoptosis protein-2 (IAP-2) proteins. Thus knockdown IAP-2 would be promising way to battle against caused by COF. Functionalized gold nanoparticle (AuNP) is an effective delivery system for bio-active materials. Here, biocompatible hyaluronic acid (HA) was fabricated into nanoparticles increase target specificity binding CD44-over-expressed cells....

Graphene-based nanocomposites such as graphene oxide (GO) and nanoparticle-decorated with demonstrated excellent physicochemical properties have worthwhile applications in biomedicine bioengineering tissue engineering. In this study, we fabricated gold GO (GO-Au) characterized their using UV-Vis absorption spectra, FTIR contact angle analyses, free radical scavenging potential. Moreover, investigated the potent of GO-Au on directing mesenchymal stem cells (MSCs) for regeneration. We compared...

A simple surface modification method, comprising of a thin coating with gold nanoparticles (AuNPs) and fibronectin (FN), was developed to improve the biocompatibility required for cardiovascular devices. The nanocomposites from FN AuNPs (FN-Au) were characterized by atomic force microscopy (AFM), UV-Vis spectrophotometry (UV-Vis), Fourier transform infrared spectroscopy (FTIR). evaluated response monocytes platelets material in vitro. FN-Au coated surfaces demonstrated low monocyte...

In this study, polyethylene glycol (PEG) with hydroxyapatite (HA), the incorporation of physical gold nanoparticles (AuNPs), was created and equipped through a surface coating technique in order to form PEG-HA-AuNP nanocomposites. The morphology chemical composition were characterized using scanning electron microscopy (SEM), atomic force (AFM), UV-Vis spectroscopy (UV-Vis), Fourier transform infrared (FTIR), X-ray photoelectron (XPS), contact angle assessment. effects nanocomposites on...

Gold nanoparticles (AuNPs) are well known to interact with cells, leading different cell behaviors such as proliferation and differentiation capacity. Biocompatibility biological functions enhanced by nanomedicine the most concerning factors in clinical approaches. In present research, AuNP solutions were prepared at concentrations of 1.25, 2.5, 5 10 ppm for biocompatibility investigations. Ultraviolet–visible spectroscopy was applied identify presence AuNPs under various concentrations....

A nanocomposite composed of polyethylene glycol (PEG) incorporated with various concentrations (~17.4, ~43.5, ~174 ppm) gold nanoparticles (Au) was created to investigate its biocompatibility and biological performance in vitro vivo. First, surface topography chemical composition determined through UV-visible spectroscopy (UV-Vis), Fourier-transform infrared (FTIR), atomic force microscopy (AFM), scanning electron (SEM), free radical scavenging ability, water contact angle measurement....

The engineering of vascular regeneration still involves barriers that need to be conquered. In the current study, a novel nanocomposite comprising fibronectin (denoted as FN) and small amount silver nanoparticles (AgNP, ~15.1, ~30.2 or ~75.5 ppm) was developed its biological function biocompatibility in Wharton’s jelly-derived mesenchymal stem cells (MSCs) rat models investigated. surface morphology well chemical composition for pure FN FN-AgNP nanocomposites incorporating various amounts...

Chitosan (Chi) is a natural material which has been widely used in neural applications due to possessing better biocompatibility. In this research study, novel of nanocomposites film based on Chi with hyaluronic acid (HA), combined varying amounts gold nanoparticles (AuNPs), was created resulting pure Chi, Chi-HA, Chi-HA-AuNPs (25 ppm), and (50 ppm).This study focused evaluating their effects mesenchymal stem cell (MSC) viability, colony formation, The surface morphology chemical position...

A fluorescein isothiocyanate (FITC)-labeled, hyaluronic acid (HA)-coated nanogld (NP-FITC) was developed to carry plasmid or siRNA into mesenchymal stem cells (MSCs). NP-FITC characterized by scanning electron microscopy (SEM), ultraviolet-visible (UV-vis) spectroscopy, and Fourier transform infrared (FTIR) spectrophotometry. Nontoxicity of in both normal cancer confirmed the MTT assay. The cellular uptake at different time points (30 min, 2 h, 24 h) verified using an immunofluorescence...

Tissue repair engineering supported by nanoparticles and stem cells has been demonstrated as being an efficient strategy for promoting the healing potential during regeneration of damaged tissues. In current study, we prepared various nanomaterials including pure Pul, Col, Pul–Col, Pul–Au, Pul–Col–Au, Col–Au to investigate their physicochemical properties, biocompatibility, biological functions, differentiation capacities, anti-inflammatory abilities through in vitro vivo assessments. The...

Cardiovascular Diseases (CVDs) such as atherosclerosis, where inflammation occurs in the blood vessel wall, are one of major causes death worldwide. Mesenchymal Stem Cells (MSCs)-based treatment coupled with nanoparticles is considered to be a potential and promising therapeutic strategy for vascular regeneration. Thus, angiogenesis enhanced by critical concern. In this study, Polyethylene Glycol (PEG) incorporated 43.5 ppm gold (Au) was prepared evaluation biological effects through vitro...

Immature platelet fraction (IPF) is a new biomarker for thrombopoiesis and inflammation. However, the reference interval (RI) wildly discrepant among published reports. This study aimed to establish RI of IPF population in Taiwan evaluate effects detection method analyzer, ethnicity, individuals have on IPF.The absolute (A-IPF) IPF% were established with healthy subjects from outpatient services Health Management Department Taichung Veterans General Hospital between January 1, 2015 March...

Background: Nanoparticles have wide potential applications in biolabeling, bioimaging, and cell tracking. Development of dual functional nanoparticles increases the versatility. Methods: We combined fluorescent property nano-epoxy (N-Epo) magnetic characteristic FePt to fabricate FePt-decorated N-Epo (N-Epo-FePt). The size diameter N-Epo-FePt (177.38 ± 39.25 nm) was bigger than (2.28 1.01 nm), both could be absorbed into mesenchymal stem cells (MSCs) via clathrin-mediated endocytosis...