The so-called "Trojan-horse" mechanism, in which nanoparticles are internalized within cells and then release high levels of toxic ions, has been proposed as a behavior the cellular uptake Ag (AgNPs). While several reports claim to have proved this mechanism by measuring AgNPs ions (I) cells, it cannot be fully proven without examining those two components both intra- extracellular media. In our study, we found that even though take up similarly (microglia (BV-2)) or more rapidly than...

The epithelial-to-mesenchymal transition (EMT) process results in a loss of cell-cell adhesion, increased cell mobility, and is crucial for enabling the metastasis cancer cells. Recently, enzyme SIRT1 has been implicated variety physiological processes; however, its role regulating oral EMT not fully elucidated. Here, we propose mechanism by which sirtuin1 (SIRT1) regulates deacetylating Smad4 repressing effect TGF-β signaling on matrix metalloproteinase-7 (MMP7). roles tumor...

<h3>Importance</h3> Statins are the drug class most commonly used to treat hyperlipidemia. Recently, they have been during pregnancy for prevention or treatment of preeclampsia. However, safety statin use has questioned, and sample sizes previous studies small. <h3>Objective</h3> To examine perinatal outcomes among offspring associated with maternal statins pregnancy. <h3>Design, Setting, Participants</h3> This retrospective cohort study included 1 443 657 pregnant women 18 years age older...

Abstract Background ADH1B rs1229984 and ALDH2 rs671 are the specifically prevalent functional variants in East Asians. These variants, which result a dramatic change enzyme activity, highly associated with alcohol‐related disorders cancer. Previous studies focusing on additive synergic effects of few inconsistent. The aim research was to evaluate associations risks disorder Methods This cohort study enrolled 42,665 participants from Taiwan Precision Medicine Initiative database, including...

Objectives This study aimed to develop a predictive model using polygenic risk score (PRS) forecast renal outcomes for adult systemic lupus erythematosus (SLE) in Taiwanese population. Methods Patients with SLE (n=2782) and matched non-SLE controls (n=11 128) were genotyped Genome-Wide TWB 2.0 single-nucleotide polymorphism (SNP) array. PRS models (C+T, LDpred2, Lassosum, PRSice-2, PRS-continuous shrinkage (CS)) constructed predicting susceptibility. Logistic regression was assessed...

Abstract In the field of lipidomics, where complexity lipid structures and functions presents significant analytical challenges, LipidSig stands out as first web-based platform providing integrated, comprehensive analysis for efficient data mining lipidomic datasets. The upgraded 2.0 (https://lipidsig.bioinfomics.org/) simplifies process empowers researchers to decipher complex nature lipids link specific characteristics biological contexts. This tool markedly enhances efficiency depth...

Abstract Background Limited evidence exists to support any specific medication over others prevent dementia in older patients with type 2 diabetes (T2D). We investigated whether treatment sodium‐glucose cotransporter (SGLT‐2) inhibitors is associated a lower risk of incident and all‐cause mortality, relative dipeptidyl peptidase‐4 (DPP‐4) glucagon‐like peptide‐1 receptor agonists (GLP‐1 RA). Methods In this retrospective, active‐comparator cohort study, we used data from the TriNetX...

Treatment of distal radial fractures that include small anterior rim fragments can be difficult. We retrospectively reviewed 19 patients in whom an plate with locking screws was used. After a median follow-up 18 months (range 6-32; interquartile range (IQR) 14, 26), the wrist flexion and extension arc 70° 50-80; IQR 60, 70), grip strength 80% contralateral side 52-104; 77, 88), visual analogue scale score for pain 0 0-5; 0, 1), disabilities arm, shoulder hand 2 0-59; 11) modified Mayo 80...

ABSTRACT Background and Objective We determined the impact of genetic susceptibility its interaction with smoking air pollution on risk developing lung adenocarcinoma. Methods This retrospective case–control study utilised data from Taiwan Precision Medicine Initiative (TPMI) project conducted between June 2019 November 2022. The population consisted adenocarcinoma patients 1:4 age‐, gender‐, index year‐matched non‐lung cancer controls. analysed polygenic scores (PRS), status, as well PM 2.5...

Endometrial cancer is the most common gynecologic malignancy in developed countries, and its incidence rising globally. Genetic predisposition plays a significant role modulating risk, particularly Asian populations. In Taiwan, burden of endometrial has increased, highlighting need to gain better understanding genetic loci associated with this disease. This retrospective case–control study included 373 patients 3730 controls from Taiwan Precision Medicine Initiative. Genotype data were...

Abstract Background Pancreatic adenocarcinoma remains a highly lethal malignancy with limited treatment options and poor survival rates. Genetic predisposition, particularly single-nucleotide polymorphisms (SNPs), may play crucial role in disease susceptibility. This study aimed to investigate the association between specific SNPs pancreatic risk Taiwanese population. Methods hospital-based retrospective analyzed genomic data from 52 individuals diagnosed at Taichung Veterans General...

Abstract Background Sirtuins (SIRT1-7) are a family of NAD-dependent deacetylases, which play an important role in regulating cancer tumorigenesis; however, their oral has been controversial. SIRT3 is localized the mitochondria, where it deacetylates and activates several enzymes involved cellular redox balance defense against oxidative damage. Results We found that compared with normal human keratinocytes (HOK), highly expressed squamous cell carcinoma (OSCC) lines, but enzymatic...

Background: Esophageal cancer has extreme worldwide demographic and histologic variations in occurrence; thus, understanding the pathogenesis of esophageal must be region- or country-based. We examined incidence tumor stage at diagnosis relation to patients’ socioeconomic status (SES) Taiwan. Method: This retrospective cohort study used data from Taiwan’s National Health Insurance Research Database Taiwan Cancer Registry collected between January 2008 December 2014. The records 40-...

A lysine racemase (lyr) gene was isolated from a soil metagenome by functional complementation for the first time using Escherichia coli BCRC 51734 cells as host and d-lysine selection agent. The lyr consisted of 1,182-bp nucleotide sequence encoding protein 393 amino acids with molecular mass about 42.7 kDa. enzyme exhibited higher specific activity toward in l-lysine-to-d-lysine direction than reverse reaction.

Abstract Penicillin allergy is a potentially life‐threatening condition that common among patients. However, the genetic associations with penicillin are not yet recognized for prevention or diagnosis, particularly in East Asian populations. We conducted retrospective case–control study using data from Taiwan Precision Medicine Initiative and analysing DNA samples to identify eight major MHC Class I II loci. employed imputation methods accurate HLA typing enrolled 17,827 individuals who...

BACKGROUND The prevalence of non-alcoholic fatty liver (NAFLD) has increased recently. Subjects with NAFLD are known to have higher chance for renal function impairment. Many past studies used traditional multiple linear regression (MLR) identify risk factors decreased estimated glomerular filtration rate (eGFR). However, medical research is increasingly relying on emerging machine learning (Mach-L) methods. present study enrolled healthy women affecting eGFR in subjects and without (NAFLD+,...

Enterovirus A71 (EV-A71) in the Picornaviridae family causes hand-foot-and-mouth disease, aseptic meningitis, severe central nervous system even death. EV-A71 2A protease cleaves Type I interferon (IFN)-α/β receptor 1 (IFNAR1) to block IFN-induced Jak/STAT signaling. This study investigated anti-EV-A7l activity and synergistic mechanism(s) of a novel furoquinoline alkaloid compound CW-33 alone combination with IFN-β Anti-EV-A71 activities were evaluated by inhibitory assays virus-induced...

ABSTRACT Areca chewing is an important environmental risk factor for development of oral premalignant lesions and cancer. Epidemiological evidence indicates that areca tightly linked to carcinogenesis. However, the pathogenetic impacts nut extract (ANE) on normal human keratinocytes (HOKs) are unclear possibly involve oxidative stress via redox imbalance. Sirtuin 3 (SIRT3) a member sirtuin family proteins play role in regulating cellular reactive oxygen species (ROS) production. Recent...

ABSTRACT SHV‐12 is the most widespread resistance determinant of Enterobacter cloacae in Taiwan; however, bla has rarely been mobilized. Six multidrug‐resistant E. isolates were collected. After conjugal transfer, plasmid profiling and analysis incompatibility groups was performed to characterize genetic context ‐containing fragments. The presence mobile elements demonstrated by PCR, cloning, sequencing bioinformatics analyses. Four different β‐lactamase genes ( TEM‐1 , CTX‐M‐3 and/or...