A5051563501- Regulation of Appetite and Obesity
- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Adipose Tissue and Metabolism
- Neurotransmitter Receptor Influence on Behavior
- Music Therapy and Health
- Sleep and Wakefulness Research
- Apelin-related biomedical research
- Neuroscience and Neuropharmacology Research
- Botulinum Toxin and Related Neurological Disorders
- Pain Management and Placebo Effect
- Circadian rhythm and melatonin
- Cancer, Stress, Anesthesia, and Immune Response
- Connective tissue disorders research
- Neurological Disease Mechanisms and Treatments
- Endoplasmic Reticulum Stress and Disease
- Neuroendocrine Tumor Research Advances
- Neuroscience of respiration and sleep
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Thyroid Cancer Diagnosis and Treatment
- Adenosine and Purinergic Signaling
- Intensive Care Unit Cognitive Disorders
- Neuroendocrine regulation and behavior
Universidade do Porto
2006-2018
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2017-2018
Instituto de Biologia Molecular e Celular
2013-2017
University of Zurich
2010-2011
Swiss Integrative Center for Human Health
2008-2010
Circulating amylin inhibits food intake via activation of the area postrema (AP). The aim this study was to identify neurochemical phenotype neurons mediating amylin's hypophagic action by immunohistochemical and feeding studies in rats. Expression c-Fos protein used as a marker for neuronal dopamine-β-hydroxylase (DBH), enzyme-catalyzing noradrenaline synthesis, noradrenergic neurons. We found that ∼50% amylin-activated AP are noradrenergic. To clarify functional role these effect on...
Peripheral amylin inhibits eating via the area postrema (AP). Because activates extracellular-signal regulated kinase 1/2 (ERK) pathway in some tissues, and because ERK1/2 phosphorylation (pERK) leads to acute neuronal responses, we postulated that it may be involved amylin's inhibitory effect. Amylin-induced ERK was investigated by immunohistochemistry brain sections containing AP. pERK-positive AP neurons were double-stained for calcitonin 1a/b receptor, which is part of functional...
Oleoylethanolamide (OEA) is a gut-derived endogenous lipid that stimulates vagal fibers to induce satiety. Our previous work has shown peripherally administered OEA activates c-fos transcription in the nucleus of solitary tract (NST) and paraventricular (PVN), where it enhances oxytocin (OXY) expression. The anorexigenic action prevented by intracerebroventricular administration selective OXY receptor antagonist, suggesting necessary role OXYergic mediation OEA's effect. NST source direct...
Background Experimental osteoarthritis entails neuropathic-like changes in dorsal root ganglia (DRG) neurons. Since glial activation has emerged as a key player nociception, being reported numerous models of neuropathic pain, we aimed at evaluating if cell may also occur the DRG and spinal cord rats with induced by intra-articular injection collagenase. Methods Osteoarthritis was two injections, separated three days, 500 U type II collagenase into knee joint rats. Movement-induced...
Abstract γ‐Aminobutyric acid type B (GABA ) receptors are involved in the modulation of neuronal activity response to chronic noxious input. However, effect their activation inflammatory pain relay thalamic nuclei such as ventrobasal complex (VB) is not known. In this study, experimental groups 2, 4, and 14 days monoarthritic (MA) rats were injected with saline (controls) or baclofen (0.875 μg), a specific GABA receptor agonist, VB contralateral inflamed joint, ankle‐bend test was performed....
Abstract Background Amylin is a calcitonin gene‐related peptide family member expressed by nociceptors. Amylin's expression down‐regulated following nerve damage, and studies suggested it affects nociception. We aimed at clarifying amylin's effects on chronic neuropathic pain investigating its site of action. Methods Chronic was induced in rats spared injury (SNI) surgery. Mechanical allodynia/hyperalgesia cold were assessed the von Frey, pinprick, acetone plate behavioural tests,...
Amylin is a peptide from the calcitonin gene-related peptides (CGRP) family that expressed by nociceptors. may modulate pain via spinal action.The effect of amylin's administration on formalin test acute and tonic was evaluated. Amylin's ability to neuronal activity analysed c-Fos expression at cord lumbar 4-5 region (L4-5) brain.Amylin subcutaneous 20 min prior, but not immediately before formalin, shortened interphase anticipated beginning phase. reduced number activated neurons. Blockade...
The role of mu-opioid receptors (MORs) in the inflammatory pain processing mechanisms within ventrobasal complex thalamus (VB) is not well understood. This study investigated effect modulating MOR activity upon nociception, by stereotaxically injecting specific ligands VB. Nociceptive behaviour was evaluated two established animal models pain, using formalin (acute and tonic pain) ankle-bend (chronic monoarthritic tests. Control (saline intra-VB injection) formalin-injected rats showed acute...
Background Calcitonin expression is a well-established marker for medullary thyroid carcinoma (MTC); yet the role of calcitonin receptor (CTR), its seven-transmembrane G-protein coupled receptor, remains to be established in C-cells derived tumors. The aim this work was investigate CTR MTC and correlate such with clinicopathological features order evaluate possible as prognostic indicator disease aggressiveness outcome. Methods analyzed series 75 MTCs by immunohistochemistry, qPCR mRNA...