A5041783599- Muscle Physiology and Disorders
- Exercise and Physiological Responses
- Autophagy in Disease and Therapy
- Protease and Inhibitor Mechanisms
- Platelet Disorders and Treatments
- Erythrocyte Function and Pathophysiology
- Single-cell and spatial transcriptomics
- Cell Adhesion Molecules Research
- Cell death mechanisms and regulation
- CAR-T cell therapy research
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Particle accelerators and beam dynamics
- Blood Coagulation and Thrombosis Mechanisms
- Muscle metabolism and nutrition
- Peptidase Inhibition and Analysis
- Signaling Pathways in Disease
- Particle Accelerators and Free-Electron Lasers
- Advanced X-ray and CT Imaging
- Advanced Radiotherapy Techniques
- Endoplasmic Reticulum Stress and Disease
- Knee injuries and reconstruction techniques
- Gyrotron and Vacuum Electronics Research
- TGF-β signaling in diseases
- Planarian Biology and Electrostimulation
Centre National de la Recherche Scientifique
2023-2024
Unit of Functional and Adaptive Biology
2023-2024
Baxter (United States)
2015-2023
Université Paris Cité
2023
Stanford University
1988-2022
Temecula Valley Unified School District
2022
Valley Hospital
2022
Valley Hospital Medical Center
2022
Stanford Medicine
2020
National Student Clearinghouse Research Center
2017
Significance Muscle repair after injury entails an immune response that orchestrates efficacious regeneration. Here we identify Prostaglandin E2 (PGE2) as a crucial inflammatory mediator of muscle stem cells (MuSCs), the building blocks PGE2 is synthesized and secreted into stem-cell niche in to injury, leading robust MuSC proliferation, key myofiber repair. EP4 receptor mediates signaling MuSCs, genetically engineered mice lack MuSCs have impaired Nonsteroidal anti-inflammatory drugs...
Maintaining muscle Prostaglandin E2 (PGE2), an eicosanoid that mediates inflammatory responses, also supports the function of stem cells. Palla et al. found loss PGE2 in aging mice contributes to and appears be a consequence increased activity 15-hydroxyprostaglandin dehydrogenase (15-PGDH), enzyme degrades (see Perspective by Becker Rudolph). Restoring concentrations inhibiting 15-PGDH older improved function. Decreased animals had beneficial effects included decreased proteolysis...
Abstract The balance between stem cell quiescence and proliferation in skeletal muscle is tightly controlled, but perturbed a variety of disease states. Despite progress identifying activators proliferation, the niche factor(s) responsible for induction remain unclear. Here we report an vivo imaging-based screen which identifies Oncostatin M (OSM), member interleukin-6 family cytokines, as potent inducer (MuSC, satellite cell) quiescence. OSM produced by fibers, induces reversible MuSC cycle...
Selenoprotein N (SEPN1) is a protein of the endoplasmic reticulum (ER) whose inherited defects originate SEPN1-related myopathy (SEPN1-RM). Here, we identify an interaction between SEPN1 and ER-stress-induced oxidoreductase ERO1A. ERO1A, both enriched in mitochondria-associated membranes (MAMs), are involved redox regulation proteins. ERO1A depletion knockout cells restores ER redox, re-equilibrates short-range MAMs, rescues mitochondrial bioenergetics. mouse background loss blunts stress...
Abstract Vascular calcifications can occur in the elderly and patients suffering from various diseases. Interestingly, depending on pathology, different regions of arterial system be affected. Embryonic observations have clearly indicated that vascular smooth muscle cell (VSMC) origin is notably heterogeneous. For instance, aorta, VSMCs colonizing aortic arch region derive cardiac neural crest cells, whereas those populating descending aorta mesoderm. We examined here whether embryonic would...
To test if proteolysis is involved in tumor cell extravasation, we developed an vitro model where cells cross endothelial monolayer cultured on a basement membrane. Using this classified the ability of to transmigrate through barrier onto underlying matrix, and scored invasion according stage passage endothelium. Metalloproteinase inhibitors reduced extravasation by at least 35%. Visualization protease adhesion molecules confocal microscopy demonstrated surface localization MMP-2, MMP-9,...
Increased pericellular proteolysis due to an imbalance between MMPs (matrix metalloproteinases) and TIMPs (tissue inhibitors of promotes early stages tumorigenesis. We have reported that TIMP-1 down-regulation confers tumorigenicity on immortal Swiss 3T3 fibroblasts. In pursuit the mechanism involved in this transformation, we asked whether MMP modulate contact inhibition cell adhesion, because dysregulation these events is essential for cellular transformation. Using both genetic...
Abstract Single-cell assays have enriched our understanding of hematopoiesis and, more generally, stem and progenitor cell biology. However, these single-end-point approaches provide only a static snapshot the state cell. To observe measure dynamic changes that may instruct fate, we developed an approach for examining hematopoietic fate specification using long-term (> 7-day) single-cell time-lapse imaging up to 13 generations with in situ fluorescence staining primary human progenitors...
Wavelength tuning of 0.4 \ensuremath{\mu}m has been obtained by the addition 100 Torr ${\mathrm{H}}_{2}$ gas to a vacuum free-electron laser operating in near infrared. This experiment demonstrates that relativistic electron beam from an rf linac can propagate partially ionized with sufficient quality achieve action. The picosecond pulse structure is believed responsible for avoidance plasma instabilities. Gain and wavelength measurements are consistent theoretical expectations.
The current model for the intrinsic apoptotic pathway holds that mitochondrial activation of caspases in response to cytotoxic drugs requires both Apaf-1-induced dimerization procaspase 9 and Smac/Diablo-mediated sequestration inhibitors apoptosis proteins (IAPs).Here, we showed either can independently promote caspase stimuli.In drug-treated Apaf-1 ؊/؊ primary myoblasts, but not fibroblasts, Smac/Diablo accumulates cytosol sequesters X-linked IAP (XIAP), which is expressed at lower levels...
The tumor suppressor Rb is thought to control cell proliferation, survival and differentiation. We recently showed that differentiating Rb-deficient mouse myoblasts can fuse form short myotubes quickly collapse through a mechanism involving autophagy, autophagy inhibitors or hypoxia could rescue the defect leading long, twitching myotubes. Here we determined contribution of pRb relatives, p107 p130, this process. show chronic acute inactivation plus p130 increased myoblast death reduced...
Abstract The proper regulation of muscle stem cell (MuSC) fate by cues from the niche is essential for regeneration skeletal muscle. How pro-regenerative factors control dynamics MuSC decisions remains unknown due to limitations population-level endpoint assays. To address this knowledge gap, we developed a dual fluorescence imaging time lapse (Dual-FLIT) microscopy approach that leverages machine learning classification strategies track single with high temporal resolution. Using two...
Assembly of the apoptosome in response to mitochondrial permeabilization, hallmark intrinsic apoptotic pathway, involves binding cytochrome c Apaf1, recruitment and auto-processing apical/signaling pro-caspase-9, coupled activation downstream/executioner caspases like caspase 3. Evidence now indicates that certain cascades can bypass activate caspase-9 independent mitochondria. Recently, we have demonstrated be activated Apaf1-mutant primary myoblasts, but not fibroblasts, stimuli are known...
A microwave field can be used to accelerate electrons as they lose energy radiation in an FEL, thereby maintaining synchronism and, conjunction with optical klystron, providing high conversion efficiencies. The microwaves programmed increase time the power level FEL so that gain is maintained over a wide range of levels. In this paper, parameter constraints for such are discussed, leading structure design integrates wiggler linac. It shown ettlciencies 50 percent at <tex...
In recent work, various design techniques were applied to investigate the feasibility of controlling bandwidth and bandshape profiles tungsten/boron-carbon (W/B4C) tungsten/silicon (W/Si) multilayers for optimizing their performance in synchrotron radiation based angiographical imaging systems at 33 keV. Varied parameters included alternative spacing geometries, material thickness ratios, numbers layer pairs. Planar optics with nominal reflectivities 30–94% bandwidths ranging from 0.6–10%...