A5081600988- Pluripotent Stem Cells Research
- Liver physiology and pathology
- Pancreatic function and diabetes
- Organ Transplantation Techniques and Outcomes
- 3D Printing in Biomedical Research
- Pediatric Hepatobiliary Diseases and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Peroxisome Proliferator-Activated Receptors
- Endoplasmic Reticulum Stress and Disease
- CRISPR and Genetic Engineering
- Protein Tyrosine Phosphatases
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Tissue Engineering and Regenerative Medicine
- Protein Degradation and Inhibitors
- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA modifications and cancer
- Autophagy in Disease and Therapy
Konkuk University
2015-2020
Recent studies have shown that defined factors could lead to the direct conversion of fibroblasts into induced hepatocyte-like cells (iHeps). However, reported efficiencies are very low, and underlying mechanism hepatic reprogramming is largely unknown. Here, we report iHeps a stepwise transition involving erasure somatic memory, mesenchymal-to-epithelial transition, induction cell fate in sequential manner. Through screening for additional potentially enhance kinetics, found c-Myc Klf4 (CK)...
Until now, there is no effective anti-fibrotic therapy available for liver cirrhosis. Stem cell therapies have been studied the treatment of hepatic fibrosis. However, use embryonic stem cells or induced pluripotent (iPSC) has limitations such as ethical concern malignancy potential. Induced hepatocyte-like (iHEPs) generated by direct reprogramming technology may overcome these limitations. In this study, we iHEPs from mouse fibroblast (MEF) either using specific transcription factors c-Myc...
Abstract The ability to generate integration-free induced hepatocyte-like cells (iHeps) from somatic fibroblasts has the potential advance their clinical application. Here, we have generated integration-free, functional and expandable iHeps mouse fibroblasts. To elicit this direct conversion, took advantage of an oriP/EBNA1-based episomal system deliver a set transcription factors, Gata4, Hnf1a Foxa3 , established exhibit similar morphology, marker expression properties primary hepatocytes....
Peroxisome proliferator–activated receptor (PPAR) δ plays a pivotal role in metabolic homeostasis through its effect on insulin signaling. Although diverse genomic actions of PPARδ are postulated, the specific molecular mechanisms whereby controls signaling have not been fully elucidated. We demonstrate here that short-term activation results formation stable complex with nuclear T-cell protein tyrosine phosphatase 45 (TCPTP45) isoform. This interaction TCPTP45 blocked translocation into...
Disorders of the biliary epithelium, known as cholangiopathies, cause severe and irreversible liver diseases. The limited accessibility bile duct precludes modeling several cholangiocyte-mediated Therefore, novel approaches for obtaining functional cholangiocytes with high purity are needed. Previous work has shown that combination Hnf1β Foxa3 could directly convert mouse fibroblasts into bipotential hepatic stem cell-like cells, termed iHepSCs. However, efficiency converting iHepSCs is low,...
Somatic cells could be directly converted into induced neural stem (iNSCs) by ectopic expression of defined transcription factors. However, the underlying mechanism direct lineage transition iNSCs is largely unknown. In this study, we examined effect genetic background on conversion process an iNSC state. The from two different mouse strains exhibited distinct efficiency as well clonal expansion. Furthermore, levels endogenous NSC markers, silencing transgenes, and in vitro differentiation...
We previously described the generation of induced hepatocyte-like cells (iHeps) using hepatic transcription factor Hnf1a together with small molecules. These iHeps represent a state that is more mature compared generated multiple factors. However, underlying mechanism conversion involving transgene dependence established largely unknown. Here, we describe transgene-independent by inducing ectopic expression both an episomal vector and doxycycline-inducible lentivirus. In contrast to...
Endoplasmic reticulum stress (ER stress) leads an unfolded protein response (UPR) which results in internal cellular responses such as proteostasis and clearance. Recently, several reports demonstrated that the ER stem cells could affect their stemness fates to differentiate into certain lineages. However, potential for controlling differentiation function of by regulating needs be further addressed. Here, we relieving cell cultures enhances functionalities hPSC-derived hepatocytes other...