A5100687973- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Probiotics and Fermented Foods
- Gut microbiota and health
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Plant Pathogens and Fungal Diseases
- Fungal Biology and Applications
- Preterm Birth and Chorioamnionitis
- Food Quality and Safety Studies
- Dental Implant Techniques and Outcomes
- Animal Virus Infections Studies
- Pharmacological Effects of Natural Compounds
- Dermatology and Skin Diseases
- Inflammatory Bowel Disease
- Enzyme Production and Characterization
- Microbial Natural Products and Biosynthesis
- Pregnancy and preeclampsia studies
- Virus-based gene therapy research
- Fungal and yeast genetics research
- Periodontal Regeneration and Treatments
- Molecular Biology Techniques and Applications
- Catalytic Processes in Materials Science
- Protein Hydrolysis and Bioactive Peptides
- Maternal and Perinatal Health Interventions
Chung-Ang University
2015-2024
Samsung Medical Center
2011-2024
Woosuk University
2014-2024
Chonnam National University
2021
Weatherford College
2020
Chung-Ang University Hospital
2020
Sungkyunkwan University
2010-2019
Harvard University
2002-2017
Seoul National University Bundang Hospital
2009-2013
Dana-Farber Cancer Institute
2009-2013
Recent studies have shown that circulating microRNAs are a potential biomarker in various types of malignancies. The aim this study was to investigate the feasibility using serum exosomal as novel serological biomarkers for hepatocellular carcinoma (HCC) patients with chronic hepatitis B (CHB). We measured and CHB (n=20), liver cirrhosis (LC) (n=20) HCC (n=20). Serum microRNA extracted from 500 μl an Exosome RNA Isolation kit. expression levels were quantified by real-time PCR. selected...
ABSTRACT Cells infected with mammalian reoviruses often contain large perinuclear inclusion bodies, or “factories,” where viral replication and assembly are thought to occur. Here, we report a strain difference in the morphology of these inclusions: filamentous inclusions formed cells reovirus type 1 Lang (T1L), whereas globular our laboratory's isolate 3 Dearing (T3D). Examination by immunofluorescence microscopy revealed be colinear microtubules (MTs). The distribution was dependent on an...
Abstract The pathogenesis of atopic dermatitis (AD) involves complex factors, including gut microbiota and immune modulation, which remain poorly understood. aim this study was to restore via fecal transplantation (FMT) ameliorate AD in mice. FMT performed using stool from donor characterized 16S rRNA sequencing analyzed Quantitative Insights into Microbial Ecology 2 with the DADA2 plugin. Gut metabolite levels were determined by measuring short-chain fatty acid (SCFA) contents. AD-induced...
The regulatory-targeting subunit (RGL, also called GM) of the muscle-specific glycogen-associated protein phosphatase PP1G targets enzyme to glycogen where it modulates activity glycogen-metabolizing enzymes. PP1G/RGL has been postulated play a central role in epinephrine and insulin control metabolism via phosphorylation RGL. To investigate function phosphatase, RGLknockout mice were generated. Animals lacking RGL show no obvious defects. is absent from skeletal cardiac muscle null mutants...
Cells infected with mammalian orthoreoviruses contain large cytoplasmic phase-dense inclusions believed to be the sites of viral replication and assembly, but morphogenesis, structure, specific functions these "viral factories" are poorly understood. Using immunofluorescence microscopy, we found that reovirus nonstructural protein microNS expressed in transfected cells forms resemble globular factories formed strain type 3 Dearing from our laboratory (T3D(N)). In cells, formation perinuclear...
The cytoplasmic TRIM5α proteins of certain mammalian lineages efficiently recognize the incoming capsids particular retroviruses and potently restrict infection in a species-specific manner. Successful have evolved that are less recognized by natural hosts. To address whether contributes to outcome retroviral susceptible host species, we investigated impact TRIM5 polymorphisms rhesus monkeys on course simian immunodeficiency virus (SIV) infection. Full-length cDNAs were derived from each 79...
This study aimed to examine the effects of Lactobacillus plantarum, a lactic acid bacteria strain isolated from kimchi, on development low-grade inflammation and type 2 diabetes mellitus (T2DM) exacerbated by chronic stress. C57BL/6 mice were fed either high-fat diet (HFD) randomized into an HFD group or that was subjected cold exposure-related stress (HFDS), normal (ND) ND (NDS). plantarum LRCC5310 (108, 1010 CFU) LRCC5314 as well L. gasseri BNR17 (108 CFU), positive control, administered...
In skeletal muscle both insulin and contractile activity are physiological stimuli for glycogen synthesis, which is thought to result in part from the dephosphorylation activation of synthase (GS). PP1G/R(GL)(G(M)) a glycogen/sarcoplasmic reticulum-associated type 1 phosphatase that was originally postulated mediate control metabolism. However, we recently showed (Suzuki, Y., Lanner, C., Kim, J.-H., Vilardo, P. G., Zhang, H., Jie Yang, J., Cooper, L. D., Steele, M., Kennedy, A., Bock,...
Mammalian reoviruses are thought to assemble and replicate within cytoplasmic, nonmembranous structures called viral factories. The nonstructural protein mu NS forms factory-like globular inclusions when expressed in the absence of other proteins binds surfaces core particles vitro. Given these previous observations, we hypothesized that one or more surface may be recruited factories through specific associations with NS. We found all three proteins--lambda 1, lambda 2, sigma 2--localized...
Abstract Aims: To test if there is a stepwise difference in neonatal outcomes according to the stage (or grade) of histological inflammatory response chorioamniotic membranes and umbilical cords preterm premature rupture (PPROM). Method: This retrospective study included singleton pregnancies diagnosed as PPROM delivered prior 34 weeks gestation (n=339). Acute chorioamnionitis funisitis were subdivided into stages defined by Redline et al. Neonatal composite morbidities mortality also...
Cutaneous wound healing comprises a complex systemic network. Probiotics, naturally extracted substances, medicine, and chemical compounds have been used for healing, but the application of postbiotics as therapeutic agents has yet to be explored. Our study shows potential beneficial effects heat-killed Lactococcus chungangensis CAU 1447 on type 1 diabetic mice. The postbiotic strain significantly decreased skin size. activity myeloperoxidase secreted from neutrophils also decreased....
Abstract The activity of the ubiquitin proteasome system (UPS) is downregulated in aggregation diseases such as Alzheimer’s disease (AD). In this study, we investigated therapeutic potential Agouti-related peptide (AgRP), which secreted by human mesenchymal stem cells (MSCs), terms its effect on regulation AD. When SH-SY5Y neuroblastoma were co-cultured with MSCs isolated from Wharton’s Jelly (WJ-MSC), their was significantly upregulated. Further analysis conditioned media after co-culture...
Abstract Atopic dermatitis (AD) has a drastic impact on human health owing to complex skin, gut microbiota, and immune responses. Some lactic acid bacteria (LAB) are effective in ameliorating AD; however, the alleviative effects of dairy products derived from these LAB remain unclear. In this study, efficacies Lactococcus chungangensis CAU 28 (CAU 28) cream cheese L . dry cells were evaluated for treating AD an mouse model. Overall, administration was more against than cells. Faeces...
The mammalian tripartite motif protein, TRIM5α, recognizes retroviral capsids entering the cytoplasm and blocks virus infection. Depending on particular TRIM5α protein retrovirus, complete disruption of RING domain decreases virus-restricting activity to various degrees. exhibits domain-dependent E3 ubiquitin ligase activity, but specific role this in viral restriction is unknown. We created a panel African green monkey (TRIM5α(AGM)) mutants, many which are specifically altered function,...
Until now, there is no effective anti-fibrotic therapy available for liver cirrhosis. Stem cell therapies have been studied the treatment of hepatic fibrosis. However, use embryonic stem cells or induced pluripotent (iPSC) has limitations such as ethical concern malignancy potential. Induced hepatocyte-like (iHEPs) generated by direct reprogramming technology may overcome these limitations. In this study, we iHEPs from mouse fibroblast (MEF) either using specific transcription factors c-Myc...
Background/Aims: This study assessed the efficacy and safety of adalimumab (ADA) explored predictors response in Korean patients with ulcerative colitis (UC).Methods: A prospective, observational, multicenter was conducted over 56 weeks adult moderately to severely active UC who received ADA. Clinical response, remission, mucosal healing were using Mayo score.Results: total 146 enrolled from 17 academic hospitals. rates 52.1% 37.7% clinical remission 24.0% 22.0% at 8 56, respectively....
Objectives. Host–microbial commensalism can shape the innate immune response in nasal mucosa, and microbial characteristics of mucus directly impact mechanisms initial allergic responses epithelium. We sought to determine alterations composition patients with rhinitis (AR) elucidate interplay between dysbiosis microbiome inflammation.Methods. In total, 364,923 high-quality bacterial 16S ribosomal RNA-encoding gene sequence reads from 104 middle turbinate mucosa samples healthy participants...
The binding stoichiometry and affinities of the Shiga toxins, Stx1 Stx2, for a series uni- oligovalent analogs Pk-trisaccharide were measured using direct electrospray ionization mass spectrometry (ES-MS) assay. Importantly, it is shown that, given ligand, Stx2 exhibit similar affinities. data suggest high degree similarity in spatial arrangement structural characteristics Pk sites Stx2. results confirm that both toxins recognize α-d-Galp(1→4)-β-d-Galp(1→4)-β-d-Glcp carbohydrate motif cell...