A5005787374- Prion Diseases and Protein Misfolding
- Neurological diseases and metabolism
- Alzheimer's disease research and treatments
- Trace Elements in Health
- Parkinson's Disease Mechanisms and Treatments
- Alcoholism and Thiamine Deficiency
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Amyotrophic Lateral Sclerosis Research
- Neurological disorders and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA regulation and disease
- Amino Acid Enzymes and Metabolism
- Complement system in diseases
- Neurological Disorders and Treatments
- Dementia and Cognitive Impairment Research
- Caveolin-1 and cellular processes
- Botulinum Toxin and Related Neurological Disorders
- Signaling Pathways in Disease
- Biochemical effects in animals
- Studies on Chitinases and Chitosanases
- Folate and B Vitamins Research
- Computational Drug Discovery Methods
- Neurological Disease Mechanisms and Treatments
- Bipolar Disorder and Treatment
- S100 Proteins and Annexins
University of Göttingen
2016-2025
Universitätsmedizin Göttingen
2016-2025
German Center for Neurodegenerative Diseases
2016-2025
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2024
Humboldt-Universität zu Berlin
2024
Freie Universität Berlin
2024
University Medical Center
2019
Universität Hamburg
2018
University Medical Center Hamburg-Eppendorf
2018
Saarland University
2018
YKL-40 (also known as Chitinase 3-like 1) is a glycoprotein produced by inflammatory, cancer and stem cells. Its physiological role not completely understood but elevated in the brain cerebrospinal fluid (CSF) several neurological neurodegenerative diseases associated with inflammatory processes. Yet precise characterization of dementia cases missing. In present study, we comparatively analysed levels CSF samples from dementias different aetiologies characterized presence cortical pathology...
Real-time quaking-induced conversion (RT-QuIC) allows the amplification of miniscule amounts scrapie prion protein (PrP(Sc)). Recent studies applied RT-QuIC methodology to cerebrospinal fluid (CSF) for diagnosing human diseases. However, date, there has not been a formal multi-centre assessment reproducibility, validity and stability in this context, an indispensable step establishment as diagnostic test clinical practice. In present study, we analysed CSF from 110 disease patients 400...
Introduction The definitive diagnosis of genetic prion diseases (gPrD) requires pathological confirmation. To date, has relied upon the finding biomarkers 14-3-3 protein and total tau (t-tau) in cerebrospinal fluid (CSF), but many researchers have reported that these markers are not sufficiently elevated gPrD, especially Gerstmann-Sträussler-Scheinker syndrome (GSS). We recently developed a new vitro amplification technology, designated "real-time quaking-induced conversion (RT-QUIC)", to...
Real-time quaking-induced conversion (RT-QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt-Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to demonstrated. Two international ring trials were undertaken in which set of 25 cerebrospinal fluid samples analyzed by total 11 different centers using range recombinant prion protein substrates instrumentation. The results show almost complete...
<h3>Objective</h3> To validate an amended protocol for clinical diagnosis of sporadic Creutzfeldt-Jakob disease (sCJD) including real-time quaking-induced conversion (RT-QuIC) and to observe its use in CJD surveillance. <h3>Methods</h3> In the framework a prospective epidemiologic study, all neuropathologically confirmed cases with sCJD who received CSF RT-QuIC analysis during diagnostic workup (n = 65) control group individuals without 118) were selected investigate accuracy protocol. The...
The development of in vitro amplification systems allows detecting femtomolar amounts prion protein scrapie (PrPSc) human cerebrospinal fluid (CSF). We performed a CSF study to determine the effects disease type, codon 129 genotype, PrPSc and other disease-related factors on real-time quaking-induced conversion (RT-QuIC) response. analyzed times 10,000 relative fluorescence units, areas under curve signal maximum RT-QuIC response as seeding parameters interest. Interestingly, type (sporadic...
Objectives Currently, the exact reasons why different α‐synucleinopathies exhibit variable pathologies and phenotypes are still unknown. A potential explanation may be existence of distinctive α‐synuclein conformers or strains. Here, we intend to analyze seeding activity dementia with Lewy bodies (DLB) Parkinson's disease (PD) brain‐derived seeds by real‐time quaking‐induced conversion (RT‐QuIC) investigate structure morphology aggregates generated RT‐QuIC. Methods misfolded...
The main objective of the present study is to therapeutic efficiency doxycycline in a double-blinded randomised phase II cohort patients with sporadic Creutzfeldt-Jakob disease (sCJD).From National Reference Center TSE Surveillance Germany, probable or definite sCJD were recruited for oral (EudraCT 2006-003934-14). In addition, we analysed data from CJD who received compassionate treatment separate group. Potential factors which influence survival such as age at onset, gender, codon 129...
The clinical diagnosis of vascular dementia (VaD) is based on imaging criteria, and specific biochemical markers are not available. Here, we investigated the potential cerebrospinal fluid (CSF) lipocalin 2 (LCN2), a secreted glycoprotein that has been suggested as mediating neuronal damage in brain injuries. study included four independent cohorts with total n = 472 samples. LCN2 was significantly elevated VaD compared to controls, Alzheimer's disease (AD), other neurodegenerative dementias,...
Abstract Introduction Neurofilament light (NFL) levels in the cerebrospinal fluid are increased several neurodegenerative dementias. However, their diagnostic accuracy differential context is unknown. Methods Cerebrospinal NFL were quantified nonprimarily neurological and psychiatric diseases (n = 122), mild cognitive impairment 48), Alzheimer's disease 108), dementia with Lewy bodies/Parkinson's 53), vascular 46), frontotemporal 41), sporadic Creutzfeldt‐Jakob (sCJD, n 132), genetic prion...
The analysis of cerebrospinal fluid biomarkers gains importance in clinical routine and is effective substantiating dementia diagnosis the differential diagnostic context.We evaluated levels β-amyloid (Aβ) 42, Aβ40, tau, P-tau a large patient population subdivided into prion diseases, tauopathies, synucleinopathies, controls. Diagnostic test evaluation was assessed by ROC area under curve analysis.High tau were detected sporadic Creutzfeldt-Jakob disease (sCJD) high Alzheimer's (AD) sCJD....
Increased plasma YKL-40 has been reported in Alzheimer's disease (AD), but its levels other neurodegenerative diseases are unknown. Here, we aimed to investigate the spectrum of dementias.YKL-40 was quantified 315 cases, including healthy controls (HC), neurological (ND), AD, vascular dementia (VaD), frontotemporal (FTD), sporadic Creutzfeldt-Jakob (CJD) and Lewy body (LBD). Diagnostic accuracy differential diagnostic context influence age gender assessed.Highest were detected CJD, followed...
Objective To investigate whether cerebrospinal fluid (CSF) neurogranin concentrations are altered in sporadic Creutzfeldt-Jakob disease (CJD), comparatively with Alzheimer’s (AD), and associated neuronal degeneration brain tissue. Methods CSF neurogranin, total tau, neurofilament light (NFL) 14-3-3 protein were measured neurological controls (NCs, n=64), AD (n=46) CJD (n=81). The accuracy of discriminating the three diagnostic groups was evaluated. Correlations between neurodegeneration...
Alpha-synuclein (aSyn) accumulates in intracellular inclusions synucleinopathies, but the molecular mechanisms leading to disease are unclear. We identify 10 kDa heat shock protein (HSP10) as a mediator of aSyn-induced mitochondrial impairments striatal synaptosomes. find an age-associated increase cytosolic levels HSP10, and concomitant decrease levels, aSyn transgenic mice. The superoxide dismutase 2, client HSP10/HSP60 folding complex, synaptosomal spare respiratory capacity also reduced....
Statins are widely prescribed drugs in cardiovascular diseases. Recent studies also demonstrated anti-inflammatory and immunomodulatory properties of statins by modulating the activity small GTPases. thus considered as potential therapeutic drug for inflammatory demyelinating disease multiple sclerosis (MS). However, little is known about effects on myelin-forming oligodendrocytes. Here, we show that hamper process myelin formation vitro interfering with Ras Rho signaling mature...
Abstract Introduction Accurate diagnosis of prion diseases and discrimination from alternative dementias gain importance in the clinical routine, but partial overlap cerebrospinal fluid (CSF) biomarkers impedes absolute differential diagnostic context. Methods We established parameters for disease quantification CSF α‐synuclein patients with sporadic ( n = 234) genetic 56) diseases, cases cognitive impairment/dementia or neurodegenerative 278), neurologic control group 111). Results An...
Abstract The objective of the study was to estimate if altered levels alpha-synuclein can be detected in tear fluid patients with Parkinson’s disease (PD). Therefore, samples 75 PD patients, control subjects and 31 atypical Parkinsonian were collected analyzed triplicates using an ultra-sensitive single molecule array (SIMOA) system applying a human immunoassay. In PD, total soluble significantly increased compared (p = 0.03; AUC vs. controls 0.60). There no difference comparing stratified...