Abstract Recent studies suggest a possible role for inhibitors of sclerostin such as antibody (Scl‐Ab) an anabolic treatment osteoporosis. Since Scl‐Ab has also been shown to potentiate bone repair, we examined the effect in metaphyseal defect repair model ovariectomized (OVX) rats. Four weeks after OVX or sham surgery, 3 mm circular defects were created bilaterally proximal tibia all After Saline 25 mg/kg was administered twice weekly weeks. Of note, healing advanced 1‐week post‐defect...

Neurofibromatosis type 1 (NF1) is a common genetic condition caused by mutations in the NF1 gene. Patients often suffer from tissue-specific lesions associated with local double-inactivation of NF1. In this study, we generated novel fracture model to investigate mechanism underlying congenital pseudarthrosis tibia (CPT) We used Cre-expressing adenovirus (AdCre) inactivate Nf1 vitro cultured osteoprogenitors and osteoblasts, vivo callus Nf1(flox/flox) Nf1(flox/-) mice. The effects presence...

Abstract An abundance of surgical studies during the past 2 centuries provide empirical evidence periosteum's regenerative power for reconstructing tissues as diverse trachea and bone. This study aimed to develop quantitative, efficacy-based measures, thereby providing translational guidelines use periosteum harness body's own healing potential generate target tissues. The current quantitatively qualitatively demonstrated tissue generation modulated by a substitute membrane that replicates...

The periosteum, a composite cellular connective tissue, bounds all nonarticular bone surfaces. Like Velcro, collagenous Sharpey's fibers anchor the periosteum in prestressed state to underlying bone. provides niche for mesenchymal stem cells. Periosteal lifting, as well injury, causes cells residing (PDCs) change from an immobile, quiescent mobile, active state. physical cues that activate PDCs home and heal injured areas remain conundrum. An understanding of these is key unlocking...

Background: Treatment of infected open fractures remains a major clinical challenge. In this study, we investigated the novel broad-spectrum antibiotic CSA-90 (cationic steroid antibiotic-90) as an antimicrobial agent. Methods: was screened in osteoblast cell culture model for effects on differentiation and mineralization. Local delivery then tested alone combination with recombinant human bone morphogenetic protein-2 (rhBMP-2) mouse ectopic formation (n = 40 mice) rat fracture inoculated...

The posterior sloping angle (PSA) has been shown to be an objective and reproducible predictor of the risk patients developing contralateral slipped capital femoral epiphysis (SCFE); however, prophylactic fixation remains controversial. This in vitro study investigates biomechanical basis using a 15-degree PSA as threshold for fixation.Synthetic bone models proximal femur were constructed with 10 degrees control (normal) group (n=6) by performing osteotomy at physis gluing head back onto...

Live and pre-recorded video tutorials are an effective means for teaching physical skills such as cooking or prototyping electronics. A dedicated cameraperson following instructor's activities can improve production quality. However, instructors who do not have access to a cameraperson's help often work within the constraints of static cameras. We present Stargazer, novel approach assisting with tutorial content creation camera robot that autonomously tracks regions interest based on...

Transforming growth factor-β (TGF-β) and bone morphogenetic proteins (BMPs) utilize parallel related signaling pathways, however the interaction between these pathways in remains unclear. TGF-β inhibition has been previously reported to promote osteogenic differentiation vitro, suggesting it may have a capacity augment orthopaedic repair. We explored this concept using an approach that represents template for testing of agents with prospective applications. The effects BMP-2, TGF-β1,...

ABSTRACT Neutralizing monoclonal sclerostin antibodies are effective in promoting bone formation at a systemic level and orthopedic scenarios including closed fracture repair. In this study we examined the effects of antibody (Scl‐Ab) treatment on regenerate volume, density, strength rat model distraction osteogenesis. Surgical osteotomy was performed 179 Sprague Dawley rats. After 1 week, rats underwent for 2 weeks, followed by 6 weeks consolidation. Two groups received biweekly...

Abstract Cemented total hip replacements generally fail after 10–20 years, often due to implant loosening from bone resorption. Bisphosphonates such as zoledronic acid (ZA) and pamidronate (PAM) are potent inhibitors of The local delivery bisphosphonates via acrylic cement could decrease osteolysis prolong lifespan. Conflicting studies suggest that bisphosphonate loading may or not reduce the mechanical properties cement. We assayed laden with ZA PAM at different concentrations diluent...

Abstract Cathepsin K inhibitors (CKIs) are an emerging class of drugs that potent antagonists osteoclastic activity. We speculated they may be beneficial in bone tissue engineering, where a stress shielded environment can lead to rapid resorption new bone. Most CKIs require frequent dosing, so achieve sustained release we manufactured polymer nanoparticles encapsulating the CKI L006235 (CKI/nP). CKI/nP and collagen matrices were used deliver them characterized by electron microscopy...

This paper compares two pattern classifiers with applications in medicine: the first is an artificial neural network weight-elimination (ANN-we); second a hybrid classifier consisting of decision-tree (DT) to eliminate variables which h

Critical-sized bone defects, whether caused by congenital malformation, tumor resection, trauma, or implant loosening, remain a major challenge for orthopaedic management. In this study we describe tissue engineering approach in mice the co-delivery of recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) and IKK inhibitor PS-1145. Scaffold implants were manufactured from poly(lactide-co-glycolide)(PLGA) Thermally-Induced Phase Separation (TIPS), with rhBMP-2 (10 μg) and PS-1145 (0...

The rate of people dying from medical errors in hospitals each year is very high. Errors that frequently occur during the course providing health care are adverse drug events and improper transfusions, surgical injuries wrong-site surgery, suicides, restraint-related or death, falls, burns, pressure ulcers, mistaken patient identities. Medical decision support systems play an increasingly important role practice. By assisting physicians making clinical decisions, improve quality care. Two...

The paper begins with a brief summary of women's involvement in science, mathematics and technology from early modern Europe to today. In the days creation technical scientific societies academies, women were completely excluded, until around middle twentieth century. post however, have participated increasingly these but this has not been at same level. Women fellows academies remain very much under-represented. A overview participation number as members level governance is presented....