A5070457334- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Periodontal Regeneration and Treatments
- Collagen: Extraction and Characterization
- Osteoarthritis Treatment and Mechanisms
- Bone health and osteoporosis research
- Bone health and treatments
- Orthopaedic implants and arthroplasty
- Wound Healing and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Cellular Mechanics and Interactions
- Dental Implant Techniques and Outcomes
- Bone and Joint Diseases
- Bone fractures and treatments
- Dermatologic Treatments and Research
- Animal Genetics and Reproduction
- Tissue Engineering and Regenerative Medicine
- Orthopedic Infections and Treatments
- Hydrogels: synthesis, properties, applications
- Bone and Dental Protein Studies
- Pluripotent Stem Cells Research
- Surgical Sutures and Adhesives
- Knee injuries and reconstruction techniques
- Hemophilia Treatment and Research
- Spinal Fractures and Fixation Techniques
University of Medicine and Health Sciences
2025
Royal College of Surgeons in Ireland
2009-2024
Trinity College Dublin
2011-2024
Advanced Materials and BioEngineering Research
2021-2023
Children's Hospital at Westmead
2013-2015
The University of Sydney
2013-2015
University College Dublin
2015
Westmead Institute
2015
Orthopaedic Research
2013
Mean pore size is an essential aspect of scaffolds for tissue-engineering. If pores are too small cells cannot migrate in towards the center construct limiting diffusion nutrients and removal waste products. Conversely, if large there a decrease specific surface area available cell attachment. However relationship between scaffold activity poorly understood as result conflicting reports within literature on optimal required successful Previous studies bone tissue-engineering have indicated...
Crosslinking and the resultant changes in mechanical properties have been shown to influence cellular activity within collagen biomaterials. With this mind, we sought determine effects of crosslinking on both compressive modulus collagen-glycosaminoglycan scaffolds osteoblasts seeded them. Dehydrothermal, 1-ethyl-3-3-dimethyl aminopropyl carbodiimide glutaraldehyde treatments were first investigated for their effect scaffolds. After this, most promising used study attachment, proliferation,...
The pore structure of three-dimensional scaffolds used in tissue engineering has been shown to significantly influence cellular activity. As the optimal size is dependant on specifics application, ability alter over a wide range essential for particular scaffold be suitable multiple applications. With this mind, aim study was develop methodologies produce collagen-glycosaminoglycan (CG) with tailored mean sizes. CG established during freeze-drying fabrication process. In study, freezing...
We have previously examined osteoblast behavior on porous collagen-glycosaminoglycan (CG) scaffolds with a range of mean pore sizes demonstrating superior cell attachment and migration in the largest pores (325 μm). Scaffolds provide framework for construct development; therefore, it is crucial to identify optimal size augmented tissue formation. Utilizing same (85 μm - 325 μm), this study aimed examine effects subsequent differentiation matrix mineralization, understand mechanism by which...
Porous collagen-glycosaminoglycan (collagen-GAG) scaffolds have shown promising clinical results for wound healing; however, these do not replace the dermal and epidermal layer simultaneously rely on local endogenous signaling to direct healing. Functionalising collagen-GAG with factors, and/or additional matrix molecules, could help overcome challenges. An ideal candidate this is platelet-rich plasma (PRP) as it a natural reservoir of growth can be activated form fibrin gel, available...
Impaired skin wound healing due to severe injury often leads dysfunctional scar tissue formation as a result of excessive and persistent myofibroblast activation, characterised by the increased expression α-smooth muscle actin (αSMA) extracellular matrix (ECM) proteins. Yet, despite extensive research on impaired advancement in tissue-engineered substitutes, remains significant clinical challenge. This study aimed first investigate effect methacrylate gelatin (GelMA) biomaterial stiffness...
Mechanical robustness is an essential consideration in the development of hydrogel platforms for bone regeneration, and despite significant advances field injectable hydrogels, many fail this regard. Inspired by mechanical properties carboxylated single wall carbon nanotubes (COOH-SWCNTs) biological advantages natural polymers, COOH-SWCNTs were integrated into chitosan collagen to formulate mechanically robust, thermoresponsive hydrogels with interconnected molecular structure load-bearing...
Treating bone infections and ensuring repair is one of the greatest global challenges modern orthopedics, made complex by antimicrobial resistance (AMR) risks due to long-term antibiotic treatment debilitating large defects following infected tissue removal. An ideal multi-faceted solution would will eradicate bacterial infection without use, simultaneously stimulating osteogenesis angiogenesis. Here, a multifunctional collagen-based scaffold that addresses these needs leveraging potential...
Abstract Osteomyelitis, a severe bone infection, is an extremely challenging complication in the repair of traumatic defects. Furthermore, use long-term high-dose antibiotics standard treatment increases risks antibiotic resistance. Herein, antibiotic-free, collagen silver-doped hydroxyapatite (coll-AgHA) scaffold reinforced with 3D printed polycaprolactone (PCL) framework was developed enhanced mechanical properties to be used load-bearing defects antimicrobial as preventative measure...
Abstract Successful therapeutic strategies for wound healing rely on proper vascularization while inhibiting fibrosis. However, scaffolds designed skin tissue engineering generally lack the biochemical cues that can enhance their without inducing Therefore, objective of this work is to incorporate platelet‐rich plasma (PRP), a natural source angiogenic growth factors, into gelatin methacrylate (GelMA) hydrogel, yielding bioink subsequently be used 3D print novel regenerative scaffold with...
Bioglasses are favorable biomaterials for bone tissue engineering; however, their applications limited due to brittleness. In addition, the early failure in interface is a common problem of composites bioglass and polymer with high mechanical strength. This effect phase separation, nonhomogeneous mixture, nonuniform strength, different degradation properties two compounds. To address these issues, this study nanoscale interaction between poly(methyl methacrylate) (PMMA) bioactive glass was...
Abstract Bisphosphonates (BP) are antiresorptive drugs with a high affinity for bone. Despite the therapeutic success in treating osteoporosis and metabolic bone diseases, chronic BP usage has been associated reduced repair of microdamage atypical femoral fracture (AFF). The latter poor prognosis, although anabolic interventions such as teriparatide (PTH(1–34)) have suggested treatment options, there is limited evidence base support their efficacy. Because PTH(1–34) acts to increase...
Sucrose acetate isobutyrate (SAIB) is a sugar-based carrier. We have previously applied SAIB as minimally invasive system for the co-delivery of recombinant human bone morphogenetic protein-2 (rhBMP-2) and found synergy when co-delivering zoledronic acid (ZA) hydroxyapatite (HA) nanoparticles. Alternative bioceramics were investigated in murine SAIB/rhBMP-2 injection model. Neither beta-tricalcium phosphate (TCP) nor Bioglass (BG) 45S5 had significant effect on volume (BV) alone or...
Bone tissue engineering approaches commonly involve the delivery of recombinant human bone morphogenetic proteins (rhBMPs).However, there are limitations associated with currently used carriers, including need for surgical implantation and increase in infection risk.As an alternative to traditional porous collagen sponge, we have adopted a solution injectable sucrose acetate isobutyrate (SAIB) as carrier rhBMP-2.The ability deliver rhBMP-2 other agents by injection reduces risk lesion size...
Activated Protein C (APC) is an anticoagulant with strong cytoprotective properties that has been shown to promote wound healing. In this study APC was investigated for its potential orthopedic application using a Bone Morphogenetic 2 (rhBMP-2) induced ectopic bone formation model. Local co-administration of 10 µg rhBMP-2 or 25 increased volume at 3 weeks by 32% (N.S.) and 74% (p < 0.01) compared alone. This associated significant increase in CD31+ TRAP+ cells tissue sections bone,...
Current clinical delivery of recombinant human bone morphogenetic proteins (rhBMPs) utilises freeze-dried collagen.Despite effective new generation, rhBMP via collagen can be limited by significant complications due to inflammation and uncontrolled formation.This study aimed produce an alternative local system permit more controllable superior rhBMP-induced formation.Cylindrical porous poly(lactic-co-glycolic acid) (PLGA) scaffolds were manufactured thermally-induced phase...