A5070022061- Trace Elements in Health
- Iron Metabolism and Disorders
- Heavy Metal Exposure and Toxicity
- Heavy metals in environment
- Environmental Toxicology and Ecotoxicology
- Neuroscience and Neuropharmacology Research
- Hormonal Regulation and Hypertension
- Metabolomics and Mass Spectrometry Studies
- Aquaculture disease management and microbiota
- Mercury impact and mitigation studies
- Drug Transport and Resistance Mechanisms
- Fish Biology and Ecology Studies
- Aquaculture Nutrition and Growth
- Diatoms and Algae Research
- Ion-surface interactions and analysis
- Electron and X-Ray Spectroscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Renal function and acid-base balance
- Agricultural Science and Fertilization
- Aldose Reductase and Taurine
- Water Quality and Pollution Assessment
- Phytase and its Applications
- Soil and Water Nutrient Dynamics
- Pesticide Exposure and Toxicity
- Advanced X-ray and CT Imaging
Imperial College London
2021-2023
London Metropolitan University
2018-2023
King's College London
2018-2023
St Thomas' Hospital
2018
Swiss Federal Institute of Aquatic Science and Technology
2009-2015
ETH Zurich
2015
Wellesley College
2009
State Street (United States)
1988
University of Mississippi Medical Center
1988
A protocol was developed to extract, fractionate, and quantitatively analyze periphyton extracellular polymeric substances (EPS), which obtains both information on the molecular weight (M r) distribution protein polysaccharide content. The EPS were extracted from freshwater between July December 2011. Organic carbon (OC) compounds different extracts analyzed using liquid chromatography-organic detection–organic nitrogen detection (LC-OCD-OND), total content quantified. Four distinct OC...
Succinate dehydrogenase is a key enzyme in the tricarboxylic acid cycle and electron transport chain. All four subunits of succinate are tumor suppressor genes predisposing to paraganglioma, but only mutations SDHB subunit associated with increased risk metastasis. Here we generated an
Zinc (Zn) has antioxidant, anti-inflammatory and anti-proliferative actions, with Zn dysregulation associated coronary ischemia/reperfusion injury smooth muscle cell dysfunction. As the majority of studies concerning have been conducted under non-physiological hyperoxic conditions, we compare effects chelation or supplementation on total intracellular content, antioxidant NRF2 targeted gene transcription hypoxia/reoxygenation-induced reactive oxygen species generation in human artery cells...
Chronic Pb exposure microcosm studies were carried out on two different periphyton communities over the course of 3 weeks to link distribution biological effects in periphyton. We show that three-week exposures 20.6 ± 0.4 μM PbT (330 nM Pb(2+)) did not have observable photosynthesis, respiration, extracellular enzymatic activities, or biomass accrual. Metal showed majority was associated with operationally defined sorbed and non-EDTA-exchangeable fractions, relatively little polymeric...
The dietary contributions of iron (Fe) and zinc (Zn) from cereals are determined by concentrations, locations chemical forms. A genetically biofortified wheat line showed higher concentrations Zn Fe than three control lines when grown over two years. mineral distributions using imaging (histochemical staining LA-ICP-MS), sequential pearling hand dissection no consistent differences between the lines. was most abundant in aleurone layer scutellum scutellar epithelium, endosperm transfer cells...
Zinc is an important component of cellular antioxidant defenses and dysregulation zinc homeostasis a risk factor for coronary heart disease ischemia/reperfusion injury. Intracellular metals, such as zinc, iron calcium are interrelated with responses to oxidative stress. Most cells experience significantly lower oxygen levels in vivo (2-10 kPa O2) compared standard vitro cell culture (18kPa O2). We report the first evidence that total intracellular content decreases human artery endothelial...
Apical uptake of zinc as ionic Zn(II) or Zn-methionine (Zn-Met) was studied in RTgutGC cell line vitro under media compositions mirroring the gut luminal concentration freshwater (FW) and seawater (SW) acclimated salmonids. Viability cells exposed to experimental preparations showed a time-dependent decrease SW treated cells, with effect being significant at 48 h (P < 0.01), but not 12 24 h. Half effective Zn exposure over (EC50, μM) differentially affected by composition (FW, 59.7 ± 12.1...
Organisms have developed metal regulatory mechanisms in response to changes the bioavailability of trace metals. Just as dictates cellular uptake, intracellular speciation determines availability metals exert biological effects. However, missing link understanding relationship between uptake and responses is ability accurately measure speciation. We conducted Pb exposure studies on well-characterized model green alga Chlamydomonas reinhardtii identified temporal under conditions relevant for...
Introduction Common variants in the SLC30A8 gene, encoding secretory granule zinc transporter ZnT8 (expressed largely pancreatic islet alpha and beta cells), are associated with altered risk of type 2 diabetes. Unexpectedly, rare loss-of-function (LoF) described heterozygous individuals only, protective against disease, even though knockout homologous gene mice leads to unchanged or impaired glucose tolerance. Here, we aimed determine how one two copies mutant R138X allele mouse impacts...
The biogeochemical cycles of lead (Pb) have been largely affected by anthropogenic activities as a result its high natural abundance and use over the centuries [1]. At sites more strongly impacted urbanization [2] mining [3], Pb is found at nano to low micromolar concentrations in surface waters, can be significantly higher soil sediment [4]. Microorganisms are everywhere their responses exposure range from resistant highly sensitive [5, 6]. These varying levels toxicity attributed cellular...
Metals and metalloids are integral to biological processes play key roles in physiology metabolism. Nonetheless, overexposure some metals or lack of others can lead serious health consequences. In this study, eight zebrafish facilities collaborated generate a multielement analysis their centralized recirculating water systems. We report first set average concentrations for 46 elements detected facilities. Our results help establish an initial baseline trouble-shooting purposes, general safe...
Assessing the availability of dietary micro-minerals is a major challenge in mineral nutrition fish species. The present article aims to describe systematic approach combining different methodologies assess zinc (Zn) Atlantic salmon (Salmo salar). Considering that several Zn chemical species can be an feed, it was hypothesised influenced by feed. Thus, this study, first protocol about how extract from feed and analyze them size exclusion chromatography-inductively coupled plasma mass...
Two PhD students reflect on their experience and impressions of the latest CH analysis 2013 conference, held at Beatenberg this past November.
<p>- Supplementary Figure 1 - 2 3 4 5 6 Table Methods -</p>
<p>Supplementary Table 2 Related to Figure 2. Differentially methylated gene-based features in Sdhb-/- versus Sdhd-/- imCCs</p>
<p>Supplementary Table 2 Related to Figure 2. Differentially methylated gene-based features in Sdhb-/- versus Sdhd-/- imCCs</p>
<p>- Supplementary Figure 1 - 2 3 4 5 6 Table Methods -</p>
<div>Abstract<p>Succinate dehydrogenase is a key enzyme in the tricarboxylic acid cycle and electron transport chain. All four subunits of succinate are tumor suppressor genes predisposing to paraganglioma, but only mutations SDHB subunit associated with increased risk metastasis. Here we generated an <i>Sdhd</i> knockout chromaffin cell line compared it <i>Sdhb-</i>deficient cells. Both types exhibited similar SDH loss function, metabolic adaptation,...
<div>Abstract<p>Succinate dehydrogenase is a key enzyme in the tricarboxylic acid cycle and electron transport chain. All four subunits of succinate are tumor suppressor genes predisposing to paraganglioma, but only mutations SDHB subunit associated with increased risk metastasis. Here we generated an <i>Sdhd</i> knockout chromaffin cell line compared it <i>Sdhb-</i>deficient cells. Both types exhibited similar SDH loss function, metabolic adaptation,...