A5020133315- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Transgenic Plants and Applications
- Biosensors and Analytical Detection
- Animal Virus Infections Studies
- Legume Nitrogen Fixing Symbiosis
- CRISPR and Genetic Engineering
- Plant Pathogenic Bacteria Studies
- Immunotherapy and Immune Responses
- Phytoplasmas and Hemiptera pathogens
- SARS-CoV-2 detection and testing
- Cancer, Stress, Anesthesia, and Immune Response
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
- vaccines and immunoinformatics approaches
- Liver physiology and pathology
- Plant responses to water stress
- Respiratory viral infections research
- Plant Physiology and Cultivation Studies
- Insect symbiosis and bacterial influences
- Soybean genetics and cultivation
Instituto Politécnico Nacional
2021-2024
Center for Research and Advanced Studies of the National Polytechnic Institute
2021-2024
Autonomous University of Tlaxcala
2022
Universidad Nacional Autónoma de México
2022
Drought is one of the main abiotic factors that affect agricultural productivity, jeopardizing food security. Modern biotechnology a useful tool for generation stress-tolerant crops, but its release and field-testing involves complex regulatory frameworks. However, gene editing technology mediated by CRISPR/Cas9 system suitable strategy plant breeding, which can lead to precise specific modifications in genome. The aim present work produce drought-tolerant varieties modifying trehalase gene....
CmNACP1 mRNA has been shown to move long distance through the phloem in Cucurbita maxima (pumpkin) and a graft junction. Whereas transport of several different mRNAs documented other systems as well, its function remains, for most these RNAs, largely unknown. To gain insight into possible role we searched closest homologs Arabidopsis, model plant much more amenable analysis. A phylogenetic approach using predicted NAC domain indicated that ANAC059, ANAC092, ANAC079, ANAC100, ANAC046, ANAC087...
The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide. Public health strategies to reduce viral transmission are based on widespread diagnostic testing detect and isolate contagious patients. Several reverse transcription (RT)-PCR tests, along with other SARS-CoV-2 assays, available attempt cover global demand. Loop-mediated isothermal amplification (LAMP) methods have been established as rapid, accurate, point care...
The Receptor-Binding Domain (RBD) of the Spike (S) protein from Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has glycosylation sites which can limit production reliable antigens expressed in prokaryotic platforms, due to glycan-mediated evasion host immune response. However, regions without glycosylated residues capable inducing neutralizing antibodies could be useful for antigen systems that do not carry machinery. To test this hypothesis, potential NG06 and NG19, located...
Insects are under constant selective pressure, which has resulted in adaptations to novel niches such as crops. This is the case of pest Melanaphis sacchari, sugarcane aphid, native Africa and currently spreading worldwide. The aphid undergoes successful parthenogenesis, causing important damage a variety crops leading economic losses for farmers. A natural M. sacchari population grown sorghum was studied identify its microbiome through sequencing 16S rDNA metagenome. high proportion...
Members of the phloem protein 16 (PP16) gene family are induced by elicitors in rice and corresponding proteins from cucurbits, which display RNA binding intercellular transport activities, accumulated sap. These facilitate movement complexes through translocation flow may be involved response to water deficit, among other functions. However, there is scant information regarding their function plants, including identification paralog genes non-vascular plants chlorophytes. In present work,...
Plant breeding plays a pivotal role in the development of improved tomato cultivars, addressing various challenges faced by this crop worldwide. Tomato yield is affected biotic and abiotic stress, including diverse pathogens pests, extreme temperatures, drought, soil salinity, thus affecting fruit quality, overall productivity. Through strategic plant approaches, it possible to increase genetic diversity leading varieties with increased resistance prevalent diseases tolerance environmental...
The plant vasculature is a central organ for long-distance transport of nutrients and signaling molecules that coordinate vegetative reproductive processes, adaptation response mechanisms to biotic abiotic stress. In angiosperms, the sieve elements are devoid nuclei, thus depending on companion cells synthesis RNA proteins, which constitute some systemic signals these processes. Massive analysis approaches have identified proteins RNAs could function as long-range in phloem translocation...
Nucleotide-binding leucine-rich repeat (NLR) plant immune receptors mediate the recognition and activation of defense signaling pathways in response to intra- extracellular pathogens. Several NLR such as Tm-2 Tm-22 have been introgressed into commercial solanaceous varieties confer protection against different tobamoviruses. Particularly, was used during recent decades resistance tobacco mosaic virus, tomato mottle virus which recognizes viral movement protein (MP). However, brown rugose...
Abstract Huanglongbing, also known as citrus greening, is currently the most devastating disease with limited success in prevention and mitigation. A promising strategy for Huanglongbing control use of antimicrobials fused to a carrier protein (phloem 16 kDa or PP16) that targets vascular tissues. This study investigated effects genetically modified trees expressing Citrus sinensis PP16 (CsPP16) human lysozyme β-defensin-2 on soil microbiome diversity using 16S amplicon analysis. The results...
The Translationally Controlled Tumor Protein (TCTP) is a multifunctional protein found in most eukaryotic taxa. In plants, TCTP regulates cell-cycle progression as well programmed cell death and involved plant regeneration responses to abiotic biotic stress, among other functions. Additionally, there evidence that the transcript are transported over long-distance. Potatoes crop of agricultural interest can reproduce asexually by from stolons tubers. Therefore, this study effect transient...
New vaccine design approaches, platforms, and immunization strategies might foster antiviral mucosal effector memory responses to reduce asymptomatic infection transmission in vaccinated individuals. Here, we investigated a combined parenteral scheme induce local serum antibody responses, employing the epitope-based antigens 3BT NG19m. These target important emerging re-emerging viruses PRRSV-2 SARS-CoV-2, respectively. We assessed two versions of protein, which contains conserved epitopes...
Abstract Although real‐time quantitative reverse transcription polymerase chain reaction (RT‐qPCR) is the gold standard for detecting virus severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) and other pathogens, disease 2019 (COVID‐19) pandemic has highlighted scarcity of instruments, devices, reagents (PCR) testing in constrained settings. At least under‐resourced countries, it become critical to deploy instruments that can be rapidly constructed satisfy this demand. Instead...
Abstract Drought is by far one of the main agricultural problems affecting crop production worldwide, generating even more economic losses than all biotic factors combined. Humankind has pursued improvement crops to enhance plant productivity under water-limiting conditions since dawn agriculture, initially through conventional breeding and recently, using tools such as transgenesis gene editing. Among editing techniques, clustered regularly interspaced short palindromic repeats...
Long-distance signaling molecules in plants, including different RNA species, play a crucial role the development and environmental responses. Among these mobile signals, Translationally Controlled Tumor Protein (TCTP) mRNA is one of most abundant. TCTP regulates cell-cycle progression programmed cell death involved responses to abiotic biotic stress as well plant regeneration, among other functions. Considering that ability induce regeneration linked possible vegetative propagation asexual...