A5075543225- Amoebic Infections and Treatments
- Parasitic Infections and Diagnostics
- Diagnosis and treatment of tuberculosis
- Pancreatitis Pathology and Treatment
- Legionella and Acanthamoeba research
- Heme Oxygenase-1 and Carbon Monoxide
- Pediatric Hepatobiliary Diseases and Treatments
- Immunotherapy and Immune Responses
- Multiple Sclerosis Research Studies
- Reproductive tract infections research
- Parasitic infections in humans and animals
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Trypanosoma species research and implications
- Herpesvirus Infections and Treatments
- Neonatal Health and Biochemistry
- Delphi Technique in Research
- Acute Myeloid Leukemia Research
- Vibrio bacteria research studies
- Escherichia coli research studies
- Research on Leishmaniasis Studies
- Adolescent Sexual and Reproductive Health
- Bacteriophages and microbial interactions
- Insect Resistance and Genetics
- Periodontal Regeneration and Treatments
Center for Research and Advanced Studies of the National Polytechnic Institute
2000-2025
Instituto Politécnico Nacional
1994-2023
Centro de Investigación en Materiales Avanzados
1991-2022
Icahn School of Medicine at Mount Sinai
2017
The University of Tokyo
2012
Instituto Nacional de Salud Pública
2004
Mexican Social Security Institute
2003
Rockefeller University
1991-1993
New York Medical College
1986
Columbia University Irving Medical Center
1986
Background: Next-generation vaccines against coronavirus disease 2019 (COVID-19) focus on inducing a long-lasting immune response severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its emerging variants. To achieve this, antigens other than spike proteins have been proposed, different platforms evaluated. Nucleic acid-based are fundamental for this process. Preclinical data shown that the SARS-CoV-2 nucleocapsid protein induces protective cellular response, when combined with...
SUMMARY Entamoeba histolytica is a human pathogen that may invade the intestinal mucosa, causing amoebic colitis or hepatic abscesses when trophozoites travel through portal circulation to liver. Lipopeptidophosphoglycan (LPPG) molecular pattern of E. recognized by immune system. Here we report LPPG exposed on cell surface trophozoites, and host toll‐like receptor (TLR) 2 TLR4. Correspondingly, embryonic kidney (HEK)‐293 cells were rendered responsive overexpression TLR2 TLR4/MD2. Moreover,...
Three-dimensional cultures are powerful tools to recapitulate animal and human tissues. Under the influence of specific growth factors, adult stem cells differentiate organize into 3D named organoids. The molecular phenotyping these structures is an essential step for validating organoid model. However, limited number organoids generated in culture yields very low amounts genetic material, making difficult. Recently, digital PCR (dPCR) techniques have become available highly sensitive...
The cytological features of Entamoeba dispar , recently recognized by biochemical and molecular biology criteria as a distinct species, were compared to those histolytica. When cultured under axenic conditions, living trophozoites E. strain SAW 760RR clone A more elongated in form, had single frontal pseudopodium, showed noticeable uroid. In sections stained with Toluidine blue, characteristic areas cytoplasmic metachromasia seen due the presence large deposits glycogen, seldom found...
ABSTRACT We have explored the biological function of a surface glycoprotein (GP72) Trypanosoma cruzi by studying null mutant parasite, generated targeted gene dele-tion. GP72 deletion affected parasite morphology in several stages life cycle. Insect midgut (epimastigote) forms had detached flagellum (apomastigote) mutant. The abnormal flagellar phenotype persisted during development infective (metacyclic) but there was no impairment aquisition complement resistance, sialidase expression or...
The objective of this study was to assess the risk infection with Giardia intestinalis in children living an area artificial groundwater recharge and potable water reuse Mexico City. Eligible wells surrounding homesteads were defined by using a geographic information system. Five tested for G. cysts per 400 liters water. A total 750 eligible households visited during two cross-sectional surveys. Stool samples provided 986 rainy season 928 dry survey parasitologic tests. Their guardians on...
ABSTRACT. The in vitro cytopathic effect of Trichomonas vaginalis on epithelial cells was explored through the interaction trophozoites virulent strain GT‐10 with MDCK monolayers. analyzed electrophysiology, video microscopy, and transmission scanning electron microscopy. Electrical measurements revealed that living parasites produced severe damage to cell monolayers within 30 min, manifested as a rapid decrease transepithelial resistance. Microscopic observations demonstrated when placed...
ABSTRACT. Sexually transmitted diseases (STDs) caused by bacteria and protozoa play an important role in the epidemiology of human immunodeficiency virus (HIV‐1) infection. Human trichomoniasis, produced protozoan parasite Trichomonas vaginalis , is one most common STDs, a cause mucosal lesions urogenital tract, which may increase risk for HIV However, there are no reports concerning outcome vitro interactions between particles trichomonads. Therefore, we incubated T. with three subtypes...
The Receptor-Binding Domain (RBD) of the Spike (S) protein from Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has glycosylation sites which can limit production reliable antigens expressed in prokaryotic platforms, due to glycan-mediated evasion host immune response. However, regions without glycosylated residues capable inducing neutralizing antibodies could be useful for antigen systems that do not carry machinery. To test this hypothesis, potential NG06 and NG19, located...
Despite all successful efforts to develop a COVID-19 vaccine, the need evaluate alternative antigens produce next-generation vaccines is imperative target emerging variants. Thus, second generation of employ more than one antigen from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induce an effective and lasting immune response. Here, we analyzed combination two SARS-CoV-2 viral that could elicit durable response in both T- B-cells. The nucleocapsid (N) protein, Spike protein...
ABSTRACT. Cysts of Naegleria fowleri present an external single-layered cyst wall. To date, little information exists on the biochemical components this Knowledge wall composition is important to understand its resistance capacity under adverse environmental conditions. We have used a monoclonal antibody (B4F2 mAb) that specifically recognizes enolase in Entamoeba invadens. By Western blot assays recognized soluble extracts N. cysts 48-kDa protein with similar molecular weight reported E....