A5072133297- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Synthesis and Characterization of Pyrroles
- Marine Sponges and Natural Products
- Ubiquitin and proteasome pathways
- Sperm and Testicular Function
- ATP Synthase and ATPases Research
- Reproductive System and Pregnancy
- Cancer therapeutics and mechanisms
- RNA modifications and cancer
- Ovarian function and disorders
- Malaria Research and Control
- Signaling Pathways in Disease
- Cancer-related Molecular Pathways
- Biochemical and Molecular Research
- Research on Leishmaniasis Studies
- Synthesis and biological activity
- Synthesis and Biological Evaluation
- Microtubule and mitosis dynamics
- Trypanosoma species research and implications
- Effects of Environmental Stressors on Livestock
- Aluminum toxicity and tolerance in plants and animals
- Retinoids in leukemia and cellular processes
- Cell death mechanisms and regulation
- Pregnancy and preeclampsia studies
Université de Lille
2013-2025
Unité de Glycobiologie Structurale et Fonctionnelle
2016-2025
Centre National de la Recherche Scientifique
2004-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022
Inserm
2007-2014
Centre de Recherche Jean Pierre Aubert
2007-2014
Laboratoire de Biologie du Développement
2011
Sorbonne Université
2011
Karolinska Institutet
2009
Université de Reims Champagne-Ardenne
2008
This study was carried out to test how sperm cryopreservation affected nuclear DNA stability and whether progeny development modified when eggs were fertilized with cryopreserved spermatozoa. The "comet assay" (alkaline single-cell gel electrophoresis assay) adapted trout spermatozoa estimate as measured by alkali-induced strand break formation. Because develop in water after fertilization (oviparous species) that eggshell is easy clear up fixative treatment, assessed from the blastodisc...
The aim of this study was to investigate the role growth hormone (GH) on in vitro cumulus expansion and oocyte maturation equine porcine cumulus-oocyte complexes (COCs), approach its way action. Equine COCs were cultured a control medium (TCM199, 5 mg/ml BSA, 1 μg/ml estradiol, antibiotics) supplemented with either 0.5 GH or LH. Porcine basal (TCM199 570 μM cysteamine) 0, 0.1, 0.5, (basal 10 ng/ml epidermal factor 400 FSH) 0 GH. After culture, nuclear stage assessed. cytoplasmic oocytes...
Abstract Lamellarin D, a potent cytotoxic marine alkaloid, exerts its antitumor action through two complementary pathways: nuclear route via topoisomerase I inhibition and mitochondrial targeting. The present study was designed to investigate the contribution of these pathways for apoptosis in cancer cells. D promoted leukemia cells without prominent cell cycle arrest. Signals transmitted by lamellarin initiated intrinsic apoptotic pathway. drug induced conformational activation Bax...
Lamellarin D (LamD) is a marine alkaloid with broad spectrum antitumor activities. Multiple intracellular targets of LamD, which affect cancer cell growth and induce apoptosis, have been identified. These include nuclear topoisomerase I, relevant kinases (such as cyclin-dependent kinase 2) the mitochondrial electron transport chain. While we previously demonstrated that LamD at micromolar range deploys strong cytotoxicity by inducing mechanisms its cytostatic effect not yet characterized....
In fully grown oocytes, meiosis is arrested at first prophase until species-specific initiation signals trigger maturation. Meiotic resumption universally involves early activation of M phase-promoting factor (Cdc2 kinase-Cyclin B complex, MPF) by dephosphorylation the inhibitory Thr14/Tyr15 sites Cdc2. However, underlying mechanisms vary. Xenopus deciphering intervening chain events has been hampered a sensitive amplification loop involving Cdc2-Cyclin B, kinase Myt1 and activating...
One of the focal subjects in insulin delivery is development formulations that protect native from degradation under acidic pH stomach. In this work we show, for first time, a graphene oxide (GO) based matrix can ensure stability at low pH. GO and modified with 2-nitrodopamine coated magnetic particle (GO–MPdop) matrices loaded were prepared triggered release was studied. The loading on nanomaterials proved to be extremely high < 5.4 capacity 100 ± 3% 88 GO–MPdop. insulin-containing stable...
Abstract Background It is clear that the coordinated and reciprocal actions of kinases phosphatases are fundamental in regulation development growth malaria parasite. Protein Phosphatase type 1 a key enzyme playing diverse essential roles cell survival. Its dephosphorylation activity/specificity governed by interaction its catalytic subunit (PP1c) with regulatory proteins. Among these, inhibitor-2 (I2) one most evolutionarily ancient PP1 regulators. In vivo studies various organisms revealed...
The aim of this study was to investigate cumulus expansion, nuclear maturation and expression connexin 43, cyclooxygenase-2 FSH receptor transcripts in equine cumuli oophori during vivo vitro the presence (eFSH) precursors for hyaluronic acid synthesis. Equine cumulus-oocyte complexes (COC) were cultured a control defined medium supplemented with eFSH (0 5 micrograms/ml), Fetal Calf Serum (FCS), synthesis or glutamine according experiments. After maturation, expansion rate increased 1...
Topoisomerases, targets of inhibitors used in chemotherapy, induce DNA breaks accumulation leading to cancer cell death. A newly synthesized copper(II) indenoisoquinoline complex WN197 exhibits a cytotoxic effect below 0.5 µM, on MDA-MB-231, HeLa, and HT-29 cells. At low doses, inhibits topoisomerase I. higher it IIα IIβ, displays intercalation properties. damage is detected by the presence γH2AX. The activation Damage Response (DDR) occurs through phosphorylation ATM/ATR, Chk1/2 kinases,...
A growing body of evidences suggests that the ovary is a site inflammatory reactions, and thus, ovarian cells could represent sources targets interleukin-1 (IL-1) system. The purpose this study was to examine IL-1 system gene expressions in equine granulosa cells, IL-1β content follicular fluid during follicle maturation. For purpose, fluids were collected from largest at early dominance stage (diameter 24 ± 3 mm) or preovulatory maturation phase, T0 h, T6 T12 T24 h T34 after induction...
The regulation of Plasmodium falciparum protein phosphatase type 1 (PfPP1) activity remains to be deciphered. Data from homologous eukaryotic phosphatases (PP1) suggest that several regulators should involved in this essential process. One such regulator, named PfI2 based on its primary sequence homology with inhibitor 2 (I2), was recently shown able interact PfPP1 and inhibit activity, mainly through the canonical 'RVxF' binding motif. details structural functional characteristics...
Novel ruthenium complexes of indenoisoquinoline derivatives were synthesized and characterized. The structure the complex 9 was determined by single-crystal X-ray crystallography. Ruthenium displayed strong DNA interactions. cytotoxic activity tested against five cancer cell lines (MDA-MB-231, MCF-7, HEK-293, HT-29, DU-145).
With its multiple regulatory partners, the conserved Protein Phosphatase type-1 (PP1) plays a central role in many functions of biology eukaryotic cells, including Plasmodium falciparum. Here, we characterized protein named PfRCC-PIP, as major partner PfPP1. We established direct interaction vitro and presence complex with PfPP1 parasite. The use Xenopus oocyte model revealed that RCC-PIP can interact endogenous PP1 act synergy suboptimal doses progesterone to trigger maturation, suggesting...
A growing body of evidence suggests that the ovary is a site inflammatory reactions, and thus, ovarian cells could represent sources targets interleukin-1 (IL-1) system. The aim present work was to investigate expression IL-1alpha, IL-1beta, IL-1ra, IL-1R1, IL-1R2 genes in equine cumulus oocytes. Moreover, influence IL-1beta on vitro maturation cumulus-oocytes complexes (COCs) examined. COCs were collected using ultrasound-guided follicular puncture vivo. Oocytes isolated from preovulatory...
Protein phosphatase 1 (PP1c) is one of the main phosphatases whose function shaped by many regulators to confer a specific location and selective for this enzyme. Here, we report that eukaryotic initiation factor 2 P. falciparum (PfeIF2β) an interactor PfPP1c. Sequence analysis PfeIF2 revealed deletion 111 amino acids when compared its human counterpart presence two potential binding motifs PfPP1 (29FGEKKK34, 103KVAW106). As expected, showed binds PfeIF2 PfeIF5, confirming canonical...