A5017875130- Regulation of Appetite and Obesity
- Neuroendocrine regulation and behavior
- Biochemical Analysis and Sensing Techniques
- Sleep and Wakefulness Research
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Hypothalamic control of reproductive hormones
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Cancer Genomics and Diagnostics
- Pharmacological Effects of Natural Compounds
- Neuroscience of respiration and sleep
- Ion channel regulation and function
- Neurobiology and Insect Physiology Research
- Cholinesterase and Neurodegenerative Diseases
- Pediatric health and respiratory diseases
- Infant Health and Development
- Lung Cancer Treatments and Mutations
- Circadian rhythm and melatonin
- Genomics and Chromatin Dynamics
- Olfactory and Sensory Function Studies
- Ion Channels and Receptors
- Memory and Neural Mechanisms
- Tuberous Sclerosis Complex Research
Florida State University
2021-2024
Heilongjiang University of Chinese Medicine
2024
GS Engineering (United States)
2024
Yale University
2012-2022
South University
2022
Central South University
2022
Erasmus University Rotterdam
2022
University of Central Florida
2022
Lanzhou University
2006-2019
University of Chicago
2012
The capacity to fix nitrogen is widely distributed in phyla of Bacteria and Archaea but has long been considered be absent from the Pseudomonas genus. We report here complete genome sequencing nitrogen-fixing root-associated stutzeri A1501. consists a single circular chromosome with 4,567,418 bp. Comparative genomics revealed that, among 4,146 protein-encoding genes, 1,977 have orthologs each five other representative species sequenced date. contains genes involved broad utilization carbon...
The neuronal substrate for binge eating, which can at times lead to obesity, is not clear. We find that optogenetic stimulation of mouse zona incerta (ZI) γ-aminobutyric acid (GABA) neurons or their axonal projections paraventricular thalamus (PVT) excitatory immediately (in 2 3 seconds) evoked binge-like eating. Minimal intermittent led body weight gain; ZI GABA neuron ablation reduced weight. generated 35% normal 24-hour food intake in just 10 minutes. cells were excited by deprivation and...
We employ transgenic mice with selective expression of tdTomato or cre recombinase together optogenetics to investigate whether hypothalamic arcuate (ARC) dopamine/tyrosine hydroxylase (TH) neurons interact other ARC neurons, how they respond neuropeptides, and test these cells constitute a single homogeneous population. Immunostaining dopamine TH antisera was used corroborate targeted transgene expression. Using whole-cell recording on large number ( n = 483), two types different...
Abstract Neurons containing melanin‐concentrating hormone ( MCH ) are located in the hypothalamus. In mice, optogenetic activation of neurons induces both non‐rapid eye movement (NREM) and rapid (REM) sleep at night, normal wake‐active period for nocturnal rodents [R. R. Konadhode et al . (2013) J. Neurosci., 33, 10257–10263]. Here we selectively activate these rats to test validity network hypothesis another species. Channelrhodopsin‐2 (ChR2) driven by promoter was expressed after injection...
Energy deprivation triggers food seeking to ensure homeostatic consumption, but the neural coding of motivational vigor in during physical hunger remains unknown. Here, we report that ablation dopamine (DA) neurons zona incerta (ZI) not ventral tegmental area potently impaired after fasting. ZI DA and their projections paraventricular thalamus (PVT) were quickly activated for approach inhibited consumption. Chemogenetic manipulation bidirectionally regulated feeding motivation control meal...
Menthol is a widely-used cooling and flavoring agent derived from mint leaves. In the peripheral nervous system, menthol regulates sensory transduction by activating TRPM8 channels residing specifically in primary neurons. Although behavioral studies have implicated actions brain, no direct central target of has been identified. Here we show that reduces excitation rat hippocampal neurons culture suppresses epileptic activity induced pentylenetetrazole injection electrical kindling vivo. We...
Oxytocin receptors (OTR) have been found in the paraventricular thalamus (PVT) for regulation of feeding and maternal behaviors. However, functional projections OTR-expressing PVT neurons remain largely unknown. Here, we used chemogenetic optogenetic tools to test role their food intake both male female OTR-Cre mice. We activation promoted seeking under trials with a progressive ratio schedule reinforcement. Using Feeding Experimentation Devices real-time meal measurements, increased...
Brain metastasis (BM) genetically diverges from the primary tumor in non‐small‐cell lung cancer (NSCLC). Hence, accurately capturing clinically relevant alterations is pivotal for delivery of targeted therapies. Circulating DNA (ctDNA) sequencing has emerged as a promising liquid biopsy biomarker‐based clinical management recurrent and extracranial metastatic NSCLC. However, absence simultaneous data brain sites prevents definitive evaluation efficacy ctDNA representing genetic profiles BM....
Thyrotropin-releasing hormone (TRH) increases activity and decreases food intake, body weight, sleep, in part through hypothalamic actions. The mechanism of this action is unknown. Melanin-concentrating (MCH) hypocretin/orexin neurons the lateral hypothalamus (LH) together with neuropeptide Y (NPY) proopiomelanocortin (POMC) arcuate nucleus play central roles energy homeostasis. Here, we provide electrophysiological evidence from GFP-reporter transgenic mouse brain slices that shows TRH...
Neurons that synthesize melanin-concentrating hormone (MCH) colocalize GABA, regulate energy homeostasis, modulate water intake, and influence anxiety, stress, social interaction. Similarly, vasopressin oxytocin can the same behaviors states, suggesting these neuropeptides may exert part of their effect by modulating MCH neurons. Using whole cell recording in MCH-green fluorescent protein (GFP) transgenic mouse hypothalamic brain slices, we found both evoked a substantial excitatory effect....
Glycine receptors (GlyRs) play important roles in regulating hippocampal neural network activity and spinal nociception. Here we show that, cultured rat (HIP) dorsal horn (SDH) neurons, 17-β-estradiol (E 2 ) rapidly reversibly reduced the peak amplitude of whole-cell glycine-activated currents ( I Gly ). In outside-out membrane patches from HIP neurons devoid nuclei, E similarly inhibited , suggesting a non-genomic characteristic. Moreover, effect on persisted presence calcium chelator...
Kisspeptin plays an important role in puberty and subsequent fertility by activating its receptor, G-protein-coupled receptor 54 (GPR54), increasing cytoplasmic free Ca(2+) concentration ([Ca(2+)](i)) gonadotropin-releasing hormone (GnRH) secretion GnRH neurons. Yet the mechanism which kisspeptin increases [Ca(2+)](i) neurons remains to be fully elucidated. In other neurons, voltage-gated channel (VGCC) activity has been shown inversely related [Ca(2+)](i). We used whole-cell patch-clamp...
Key points In conjunction with fast amino acid transmitters, neuropeptides such as dynorphin play key roles in regulating energy homeostasis. Dynorphin immunoreactive axons are abundant the hypothalamic arcuate nucleus and terminate near or on proopiomelanocortin (POMC) neurons. directly inhibits POMC cells by a mechanism based activation of GIRK channels, mediated kappa‐2 opioid receptors. indirectly modulates activity neurons depressing afferent glutamate GABA synaptic neurotransmission....
Key points Excitatory glutamate neurons are sparse in the rostral hypothalamic arcuate nucleus (ARC), subregion that has received most attention past. In striking contrast, excitatory far more common (by a factor of 10) caudal ARC, an area which relatively little attention. These cells may play negative role energy balance and food intake. They can show increase phosphorylated Stat‐3 presence leptin, electrically excited by anorectic neuromodulator cholecystokinin, inhibited orexigenic...
In the mammalian cortex, α2 subunit-containing glycine receptors (GlyRs) mediate tonic inhibition, but precise functional role of this type GlyRs is difficult to establish because lack subtype-selective antagonist. study, we found that cyclothiazide (CTZ), an epileptogenic agent, potently inhibited GlyR-mediated current (<i>I</i><sub>Gly</sub>) in cultured rat hippocampal neurons. The inhibition was concentration-dependent, suggesting a competitive mechanism. Note containing not α1 or α3...
Rostral zona incerta (ZIR) evokes feeding by sending GABA transmission to paraventricular thalamus (PVT). Although central serotonin (5-HT) signaling is known play critical roles in the regulation of food intake and eating disorders, it remains unknown whether raphe 5-HT neurons functionally innervate ZIR-PVT neural pathway for control. Here, we sought reveal how regulates both ZIR PVT We used retrograde tracers map projections Sert-Cre mice slice electrophysiology examine mechanism which...
Paraventricular thalamus (PVT) plays important roles in the regulation of emotion and motivation through connecting many brain structures including midbrain limbic system. Although acetylcholine (ACh) neurons were reported to send projections PVT, little is known about how cholinergic signaling regulates PVT neurons. Here, we used both RNAscope slice patch-clamp recordings characterize receptor expression ACh modulation mice. We found excited a majority anterior (aPVT) but predominantly...
Abstract Paraventricular thalamus (PVT) is a midline thalamic area that receives dense GABA projections from zona incerta (ZI) for the regulation of feeding behaviours. Activation central serotonin (5‐HT) signalling known to inhibit food intake. Although previous studies have reported both 5‐HT fibres and receptors in PVT, it remains unknown how regulates PVT neurons whether involved control Using slice patch‐clamp recordings combination with optogenetics, we found not only directly excited...
Energy deprivation triggers food seeking to ensure homeostatic consumption, but the neural coding of motivational vigor in during physical hunger remains unknown. Here, we report that ablation dopamine (DA) neurons zona incerta (ZI) not ventral tegmental area potently impaired after fasting. ZI DA were quickly activated for approach inhibited consumption. Chemogenetic manipulation bidirectionally regulated feeding motivation control meal frequency size intake. In addition, activation and...